Nuclear Factor 1 (NFIA) (NM_001134673) Human Untagged Clone

CAT#: SC326007

NFIA (untagged)-Human nuclear factor I/A (NFIA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Nuclear Factor 1
• Synonyms: BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001134673 edited
  ATGTATTCTCCGCTCTGTCTCACCCAGGATGAATTTCATCCTTTCATCGAAGCACTTCTG
  CCCCACGTCCGAGCCTTTGCCTACACATGGTTCAACCTGCAGGCCCGAAAACGAAAATAC
  TTCAAAAAACATGAAAAGCGTATGTCAAAAGAAGAAGAGAGAGCCGTGAAGGATGAATTG
  CTAAGTGAAAAACCAGAGGTCAAGCAGAAGTGGGCATCTCGACTTCTGGCAAAGTTGCGG
  AAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTACAGTTACAGGGAAAAAACCT
  CCATGTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGAAGAATTGACTGCCTC
  CGCCAGGCAGATAAAGTCTGGAGGTTGGACCTTGTTATGGTGATTTTGTTTAAAGGTATT
  CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGTCCCCACAATGCTCTAATCCAGGG
  CTCTGTGTCCAACCCCATCACATAGGGGTTTCTGTTAAGGAACTCGATTTATATTTGGCA
  TACTTTGTGCATGCAGCAGATTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGACGCT
  GACATTAAGGACCAGCCAGAAAATGGACATTTGGGCTTCCAGGACAGTTTTGTCACATCA
  GGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGACACCAATAGCTGCAGGAACT
  GGCCCAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTACAGCATGAGTCCAGGA
  GCAATGAGGAGGTCTTTACCCAGCACATCCTCTACGAGCTCCACAAAGCGCCTCAAGTCT
  GTGGAGGATGAAATGGACAGTCCTGGTGAGGAGCCATTTTATACAGGCCAAGGGCGCTCC
  CCAGGAAGTGGCAGTCAGTCAAGTGGATGGCATGAAGTGGAGCCAGGAATGCCATCTCCA
  ACCACACTGAAGAAGTCGGAGAAGTCTGGTTTCAGCAGCCCCTCCCCTTCACAGACCTCC
  TCCCTGGGAACGGCGTTCACACAGCATCACCGACCTGTCATTACAGGACCCAGAGCAAGT
  CCGCATGCAACACCATCGACTCTTCATTTCCCGACATCACCCATTATCCAGCAGCCTGGG
  CCTTACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACGCTGAAAGAATTTGTC
  CAACTTGTCTGCCCTGATGCTGGTCAGCAGGCTGGACAGGTGGGGTTCCTCAATCCCAAT
  GGGAGCAGCCAAGGCAAGGTGCACAACCCATTCCTTCCCACCCCAATGTTGCCACCGCCA
  CCGCCACCACCGATGGCCAGGCCTGTGCCTCTGCCGGTGCCAGACACAAAGCCTCCAACC
  ACGTCAACAGAAGGAGGTGCAGCCTCCCCCACGTCACCAACCTACTCGACACCCAGCACC
  TCCCCCGCAAACCGATTCGTCAGTGTTGGACCACGGGATCCAAGCTTTGTAAATATCCCT
  CAACAGACACAGTCCTGGTACCTGGGATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001134673
• Insert Size: 9495 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134673.2, NP_001128145.1
• RefSeq Size: 9495 bp
• RefSeq ORF: 9495 bp
• Locus ID: 4774
• UniProt ID: Q12857
• Cytogenetics: 1p31.3
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.