APLP2 (NM_001142276) Human Untagged Clone

CAT#: SC325316

APLP2 (untagged)-Human amyloid beta (A4) precursor-like protein 2 (APLP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APLP2
• Synonyms: APLP-2; APPH; APPL2; CDEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325316 representing NM_001142276.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCCACCGGGACCGCGGCCGCCGCAGCCACGGGCAGGCTCCTGCTTCTGCTGCTGGTGGGGCTC
  ACGGCGCCTGCCTTGGCGCTGGCCGGCTACATCGAGGCTCTTGCAGCCAATGCCGGAACAGGATTTGCT
  GTTGCTGAGCCTCAAATCGCAATGTTTTGTGGGAAGTTAAATATGCATGTGAACATTCAGACTGGGAAA
  TGGGAACCTGATCCAACAGGCACCAAGAGCTGCTTTGAAACAAAAGAAGAAGTTCTTCAGTACTGTCAG
  GAGATGTATCCAGAGCTACAGATCACAAATGTGATGGAGGCAAACCAGCGGGTTAGTATTGACAACTGG
  TGCCGGAGGGACAAAAAGCAATGCAAGAGTCGCTTTGTTACACCTTTCAAGTGTCTCGTGGGTGAATTT
  GTAAGTGATGTCCTGCTAGTTCCAGAAAAGTGCCAGTTTTTCCACAAAGAGCGGATGGAGGTGTGTGAG
  AATCACCAGCACTGGCACACGGTAGTCAAAGAGGCATGTCTGACTCAGGGAATGACCTTATATAGCTAC
  GGCATGCTGCTCCCATGTGGGGTAGACCAGTTCCATGGCACTGAATATGTGTGCTGCCCTCAGACAAAG
  ATTATTGGATCTGTGTCAAAAGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAAGATGAAGAG
  GAAGACTATGATGTTTATAAAAGTGAATTTCCTACTGAAGCAGATCTGGAAGACTTCACAGAAGCAGCT
  GTGGATGAGGATGATGAGGATGAGGAAGAAGGGGAGGAAGTGGTGGAGGACCGAGATTACTACTATGAC
  ACCTTCAAAGGAGATGACTACAATGAGGAGAATCCTACTGAACCCGGCAGCGACGGCACCATGTCAGAC
  AAGGAAATTACTCATGATGTCAAAGCTGTCTGCTCCCAGGAGGCGATGACGGGGCCCTGCCGGGCCGTG
  ATGCCTCGTTGGTACTTCGACCTCTCCAAGGGAAAGTGCGTGCGCTTTATATATGGTGGCTGCGGCGGC
  AACAGGAACAATTTTGAGTCTGAGGATTATTGTATGGCTGTGTGTAAAGCGATGATTCCTCCAACTCCT
  CTGCCAACCAATGATGTTGATGTGTATTTCGAGACCTCTGCAGATGATAATGAGCATGCTCGCTTCCAG
  AAGGCTAAGGAGCAGCTGGAGATTCGGCACCGCAACCGAATGGACAGGGTAAAGAAGGAATGGGAAGAG
  GCAGAGCTTCAAGCTAAGAACCTCCCCAAAGCAGAGAGGCAGACTCTGATTCAGCACTTCCAAGCCATG
  GTTAAAGCTTTAGAGAAGGAAGCAGCCAGTGAGAAGCAGCAGCTGGTGGAGACCCACCTGGCCCGAGTG
  GAAGCTATGCTGAATGACCGCCGTCGGATGGCTCTGGAGAACTACCTGGCTGCCTTGCAGTCTGACCCG
  CCACGGCCTCATCGCATTCTCCAGGCCTTACGGCGTTATGTCCGTGCTGAGAACAAAGATCGCTTACAT
  ACCATCCGTCATTACCAGCATGTGTTGGCTGTTGACCCAGAAAAGGCGGCCCAGATGAAATCCCAGGTG
  ATGACACATCTCCACGTGATTGAAGAAAGGAGGAACCAAAGCCTCTCTCTGCTCTACAAAGTACCTTAT
  GTAGCCCAAGAAATTCAAGAGGAAATTGATGAGCTCCTTCAGGAGCAGCGTGCAGATATGGACCAGTTC
  ACTGCCTCAATCTCAGAGACCCCTGTGGACGTCCGGGTGAGCTCTGAGGAGAGTGAGGAGATCCCACCG
  TTCCACCCCTTCCACCCCTTCCCAGCCCTACCTGAGAACGAAGGATCTGGAGTGGGAGAGCAGGATGGG
  GGACTGATCGGTGCCGAAGAGAAAGTGATTAACAGTAAGAATAAAGTGGATGAAAACATGGTCATTGAC
  GAGACTCTGGATGTTAAGGAAATGATTTTCAATGCCGAGAGAGTTGGAGGCCTCGAGGAAGAGCGGGAA
  TCCGTGGGCCCACTGCGGGAGGACTTCAGTCTGAGTAGCAGTGCTCTCATTGGCCTGCTGGTCATCGCA
  GTGGCCATTGCCACGGTCATCGTCATCAGCCTGGTGATGCTGAGGAAGAGGCAGTATGGCACCATCAGC
  CACGGGATCGTGGAGGTTGATCCAATGCTCACCCCAGAAGAGCGTCACCTGAACAAGATGCAGAACCAT
  GGCTATGAGAACCCCACCTACAAATACCTGGAGCAGATGCAGATTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142276
• Insert Size: 2256 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142276.1
• RefSeq Size: 3791 bp
• RefSeq ORF: 2256 bp
• Locus ID: 334
• UniProt ID: Q06481
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 85.5 kDa
• Gene Summary: This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2 which lacks an internal segment in the C-terminal region, compared to isoform 1.