SKA1 (NM_145060) Human Untagged Clone

CAT#: SC324643

SKA1 (untagged)-Human spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKA1
• Synonyms: C18orf24
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145060.1 CGGAAGCATGGATGTGCGCCTGCGCTGCGCTAGGGCGCGGCGGGCGGTTTGAATTTTGCT
  TACAGAGTCCCGTCTCACCATCCTGGGCTTCCAACGGAGACTGCGGTATCCGCGGCTGGA
  GACCCAGCGGCGAGTAGCCTTTTGCTCCCGGACGGACTTGAGAGGCTTAAAGGATGGCCT
  CGTCAGATCTGGAACAATTATGCTCTCATGTTAATGAAAAGATTGGCAATATTAAGAAAA
  CCTTATCATTAAGAAACTGTGGCCAGGAACCTACCTTGAAAACTGTATTAAATAAAATAG
  GAGATGAGATCATTGTAATAAATGAACTTCTAAATAAATTGGAATTGGAAATTCAGTATC
  AAGAACAAACCAACAATTCACTCAAGGAACTCTGTGAATCTCTTGAAGAAGATTACAAAG
  ACATAGAACATCTTAAAGAAAACGTTCCTTCCCATTTGCCTCAAGTAACAGTAACCCAGA
  GCTGTGTTAAGGGATCAGATCTTGATCCTGAAGAACCAATCAAAGTTGAAGAACCTGAAC
  CCGTAAAGAAGCCTCCCAAAGAGCAAAGAAGTATTAAGGAAATGCCATTTATAACTTGTG
  ATGAGTTCAATGGTGTTCCTTCGTACATGAAATCCCGCTTAACCTATAATCAAATTAATG
  ATGTTATTAAAGAAATCAACAAGGCAGTAATTAGTAAATATAAAATCCTACATCAGCCAA
  AAAAGTCTATGAATTCTGTGACCAGAAATCTCTATCACAGATTTATTGATGAAGAAACGA
  AGGATACCAAAGGTCGTTATTTTATAGTGGAAGCTGACATAAAGGAGTTCACAACTTTGA
  AAGCTGACAAGAAGTTTCACGTGTTACTGAATATTTTACGACACTGCCGGAGGCTATCAG
  AGGTCCGAGGGGGAGGACTTACTCGTTATGTTATAACCTGAGTCCCTTGTGAACTTTTGA
  ACATACCAACAGGGTATAGAGTATAGAGGCTATTTCTATAATTTTCTTATATATAATTTT
  TTTAACTTTTAATCTTTTTTGTTTCCTTTTTTTTTTTTTGAGACAGGATCTTGCTTTGTC
  ACCCAGGGGCTTGCTTTGTCACGCAGGCTAGAGTGCAGTGGCGCAAACATGGCTCACTGC
  AGCCTCAACCTCCCAGGCTCAAGTGATCCTCCCACCTCAGCCCCCTGAATGGCTGGGACT
  ACAAGCGTGCGCCACCATGCCTGGCTAATTTTTGTATTTTTTGGAGAGATGGGGTTTCAC
  CATGTTGCCTAGGCTGGTCTTGAGCTCCTGAGCTCAAACAATCCACCCTCCTCAGCCTCC
  CAAAGTGCTGGGATTACAGGCTTGAGCCACCACACCTGACCTATTCTTGTTTCTTATAAA
  AATAAAACTTTTTTGGATAAAGCTTATTTCTTGTTTTTTTCTTTTTCTTTTTTTTTTTTT
  GAGACAGTCTCGCTCTTTCACCCAGGCCGGACTGCAGCGGCGCTATCTCGGCTCGCTGCA
  AACTCTGCCTCCCAGGTTCACGCCATCCTCCTGCCTCAGCTACTCAGGAGAATTGCTTGA
  ACCTGGGAGGTGGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGTCTGGGCAA
  CAGAGCGAGACTCCATCTCAGAAAAAAAGAAAAAAAGACTGGGTACAGATGTGATATTGG
  AAGAAAAAGATCAAGCTGATGAGGTTAGGATACCCAGGCCCTTTGGACTTAAAGATCACT
  AGTGTCTAAATTCCATCGATGGCATTTCAGTCTATAGGTAAACTTCCTGGAAGCTGGATT
  TGGAGACAGTTTATCATCTGATTATTGGGCTTTCGTATAGGTCCTTAGGGAGCAGCTTAC
  CTGAAATGCATTTAGTGTACACCAGTCTGTAAACTTCAACCTGTAATGAAAGTGTAATAA
  ATGTACATTGAGTTGATGTGATAATGTGATATAATAAGAAATATATATTTGATCTTCCTA
  TCTAGTTCCTTGTTCAGAGCTCCTAAAACCCTTGTAATTTCCAAAGTGATGGAGTACATC
  TTTTGTTCTAGTATTTGGTCTTTGACCCCAGTTCCTGACACAAAGCTCCTAAATTCCTTT
  AAATTTCCCAGTGATAGGAGAATTTTTTGTTCTAATGAGGTCACTCTTGATGGGCACCTG
  GATAACTCAGGATGGGGGCTGCTCACAAAGACCACATCATGATTGGAAGTTTCAAACTTT
  CAGTCTCCCACCTCCAGAGAGGGGAGAGGGGCTGGAGATTTGTGTCAATAATCCATCAGG
  CCTATGTCAACAAGACATAATCCGTTAACTATGGAGTTCAGGGAGCTTCAGGGTTGGCAA
  ACATTTTGATGTGCCAGGAAGGTGACGCACTCCAGCTTTATGAAGTCAGCAAGTCCTGTG
  CTCAGGATGCTTCTGGACCTTGCCCCAGGTACCCCTTCATGTGGCTGTTGTTCATCTGTA
  TCCTTTGTAGTAGCCTTAAAATAAACTGTTAAACACAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_145060
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145060.1, NP_659497.1
• RefSeq Size: 2511 bp
• RefSeq ORF: 768 bp
• Locus ID: 220134
• UniProt ID: Q96BD8
• Cytogenetics: 18q21.1
• Gene Summary: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020). In the complex, it mediates the interaction with microtubules (PubMed:19289083, PubMed:23085020).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.