AATF (NM_012138) Human Untagged Clone

CAT#: SC324593

AATF (untagged)-Human apoptosis antagonizing transcription factor (AATF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AATF
• Synonyms: BFR2; CHE-1; CHE1; DED
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012138.3 TCCGCGCGGTCTCTGGCCGAGTCGGGGAATCGGATCAAGGCGAGAGGATCCGGCAGGGAA
  GGAGCTTCGGGGCCGGGGGTTGGGCCGCACATTTACGTGCGCGAAGCGGAGTGGACCGGG
  AGCTGGTGACGATGGCGGGGCCGCAGCCCCTGGCGCTGCAACTGGAACAGTTGTTGAACC
  CGCGACCAAGCGAGGCGGACCCTGAAGCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGA
  TTGACAGGTTTGATGAAGGGGAAGATGGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTA
  GAAAACTGGCATCAGCCTCCCTCTTGGACACGGACAAAAGGTATTGCGGCAAAACCACCT
  CTAGAAAAGCATGGAATGAAGACCATTGGGAGCAGACTCTGCCAGGATCGTCTGATGAGG
  AAATATCTGATGAGGAAGGGTCTGGAGATGAAGATTCAGAGGGACTGGGTCTGGAGGAAT
  ATGATGAGGACGACCTGGGTGCTGCTGAGGAACAGGAGTGTGGTGATCACAGGGAGAGCA
  AGAAGAGCAGAAGCCACTCTGCAAAAACACCGGGCTTCAGTGTCCAGAGTATCAGTGACT
  TTGAGAAATTTACCAAGGGAATGGATGACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAG
  AGAGTGGCATGGAAGAAGGGGATGACGCGGAAGACTCCCAAGGCGAGAGTGAGGAAGACA
  GGGCTGGAGATAGAAACAGTGAGGATGATGGTGTGGTGATGACCTTCTCTAGTGTCAAAG
  TTTCTGAGGAAGTGGAGAAAGGAAGAGCCGTGAAGAACCAGATAGCACTGTGGGACCAGC
  TCTTGGAAGGAAGGATCAAACTACAAAAAGCTCTGTTGACCACCAACCAGCTTCCTCAAC
  CAGATGTTTTCCCATTGTTCAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAA
  ATAGTCACAAGGCACTTAAAGCATTGTTGAGGTCATTGGTAGGTCTTCAGGAAGAGTTGC
  TTTTCCAGTACCCAGACACTAGATATCTAGTAGATGGGACAAAGCCCAATGCGGGAAGTG
  AGGAGATTTCTAGTGAAGATGATGAGCTGGTAGAAGAGAAGAAGCAGCAACGAAGAAGGG
  TCCCTGCAAAGAGGAAGCTGGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTG
  CCGACTTTACAGTCTACAGGAACCGCACACTTCAGAAATGGCACGATAAGACCAAACTGG
  CTTCTGGAAAACTGGGGAAGGGTTTTGGTGCCTTTGAACGCTCAATCTTGACTCAGATCG
  ACCATATTCTGATGGACAAAGAGAGATTACTTCGAAGGACACAGACCAAGCGCTCTGTCT
  ATCGAGTTCTTGGCAAACCTGAGCCAGCAGCTCAGCCTGTCCCAGAGAGTTTGCCAGGGG
  AACCGGAGATCCTTCCTCAAGCCCCTGCTAATGCTCATCTGAAGGACTTGGATGAAGAAA
  TCTTTGATGATGATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAACGGAAGACCA
  GCTCCTTGGATCCCAACGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAGT
  TACGAAGCAAAATCCACAAAAAAGTAGATAGGAAAGCCAGCAAAGGCAGGAAACTTCGGT
  TTCATGTCCTTAGCAAGCTACTGAGTTTCATGGCACCTATTGACCATACTACAATGAATG
  ATGATGCCAGGACAGAACTGTACCGCTCTCTTTTTGGCCAGCTCCACCCTCCCGACGAAG
  GCCACGGGGATTGACATCGCCCACCTCCGACACCCAGTGGGCGCCTTGGCTGGTGCGGCT
  GCTGGTCCAGATGGAGGAAACCAGTGACTTTATGGGGCTGAGCTAGTAGGGAAGCCCCTG
  GAAAGATGCTGCGTTCCGAACCTGTGCCTAATACACGCAAGGGCGCTGTCCCGCCCAACC
  CCGCCTTTAAACGCCACAAATAAAGAGCATTGTTACCGCCAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012138
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012138.3, NP_036270.1
• RefSeq Size: 2172 bp
• RefSeq ORF: 1683 bp
• Locus ID: 26574
• UniProt ID: Q9NY61
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]