SHKBP1 (NM_138392) Human Untagged Clone

CAT#: SC324510

SHKBP1 (untagged)-Human SH3KBP1 binding protein 1 (SHKBP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHKBP1
• Synonyms: PP203; Sb1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138392.1 GGGGGGCTCGCCCGGGGGCCATGGCAGCAGCGGCTACTGCAGCCGAGGGGGTCCCCAGTC
  GGGGGCCTCCCGGGGAAGTCATCCATCTGAATGTGGGAGGCAAGAGATTCAGTACCTCTC
  GCCAGACTCTCACCTGGATCCCAGACTCCTTCTTCTCCAGTCTTCTGAGCGGACGCATCT
  CGACGCTGAAAGATGAGACCGGAGCAATCTTCATCGACAGGGACCCTACAGTCTTCGCCC
  CCATCCTCAACTTCCTGCGCACCAAAGAGTTGGATCCCAGGGGTGTCCACGGTTCCAGCC
  TCCTCCATGAAGCCCAGTTCTATGGGCTCACTCCTCTGGTTCGTCGCCTGCAGCTTCGAG
  AGGAGTTGGATCGATCTTCTTGTGGAAACGTCCTCTTCAATGGTTACCTGCCGCCACCAG
  TGTTCCCAGTGAAGCGGCGGAACCGGCACAGCCTAGTGGGGCCTCAGCAGCTAGGAGGAC
  GGCCAGCCCCTGTCCGACGGAGCAACACGATGCCCCCCAACCTTGGCAATGCAGGGCTGC
  TGGGCCGAATGCTGGATGAGAAAACCCCTCCCTCACCCTCAGGACAACCTGAGGAGCCGG
  GGATGGTGCGCCTGGTGTGTGGACACCATAATTGGATCGCTGTGGCCTATACCCAGTTTC
  TAGTCTGCTACAGGTTGAAGGAAGCCTCTGGCTGGCAGCTGGTGTTTTCCAGCCCCCGCC
  TGGACTGGCCCATCGAACGACTGGCGCTCACAGCCCGGGTGCATGGTGGGGCTTTGGGTG
  AACATGACAAGATGGTGGCAGCAGCCACCGGCAGCGAGATCCTGCTATGGGCTCTGCAGG
  CGGAAGGCGGTGGCTCCGAGATAGGGGTCTTTCATCTGGGGGTGCCTGTGGAGGCCTTGT
  TCTTCGTCGGGAACCAGCTCATTGCTACAAGCCACACAGGGCGCATCGGGGTGTGGAATG
  CCGTCACCAAGCACTGGCAGGTCCAGGAGGTGCAGCCCATCACCAGTTATGACGCGGCAG
  GCTCCTTCCTCCTCCTGGGCTGCAACAACGGCTCCATTTACTACGTGGATGTGCAGAAGT
  TCCCCTTGCGCATGAAAGACAACGACCTCCTTGTCAGCGAGCTCTATCGGGACCCAGCGG
  AGGATGGGGTCACCGCCCTCAGTGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACT
  GGATCGAGATCGCCTATGGCACCAGCTCAGGGGGCGTGCGGGTCATCGTGCAGCACCCGG
  AGACTGTGGGCTCGGGGCCTCAGCTCTTCCAGACCTTCACTGTGCACCGCAGCCCTGTCA
  CCAAGATCATGCTGTCGGAGAAGCACCTCATCTCAGTCTGTGCCGACAACAACCACGTGC
  GGACATGGTCTGTGACTCGCTTCCGCGGCATGATTTCCACCCAGCCCGGCTCCACCCCAC
  TCGCTTCCTTTAAGATCCTGGCTCTGGAGTCGGCAGATGGGCATGGCGGCTGCAGTGCTG
  GCAATGACATTGGCCCCTACGGTGAGCGGGACGACCAGCTAGTGTTCATCCAGAAGGTGG
  TGCCCAGTGCCAGCCAGCTCTTCGTGCGTCTCTCATCTACTGGGCAGCGGGTGTGCTCCG
  TGCGCTCCGTGGACGGCTCACCCACGACGGCCTTCACAGTGCTGGAGTGCGAGGGCTCCC
  GGCGGCTCGGCTCTCGGCCCCGGCGCTACCTGCTCACTGGCCAGGCCAACGGCAGCTTGG
  CCATGTGGGACCTAACCACCGCCATGGACGGCCTCGGCCAGGCCCCTGCAGGTGGCCTGA
  CGGAGCAAGAGCTGATGGAACAGCTGGAACACTGTGAGCTGGCCCCGCCGGCTCCTTCAG
  CTCCCTCATGGGGCTGTCTCCCCAGCCCCTCACCCCGCATCTCCCTCACCAGCCTCCACT
  CAGCCTCCAGCAACACCTCCTTGTCTGGCCACCGTGGGAGCCCAAGCCCCCCGCAGGCTG
  AGGCCCGGCGCCGTGGTGGGGGCAGCTTTGTGGAACGCTGCCAGGAACTGGTGCGGAGTG
  GGCCAGACCTCCGACGGCCACCCACACCAGCCCCGTGGCCCTCCAGCGGTCTCGGCACTC
  CCCTCACACCTCCCAAGATGAAGCTCAATGAAACTTCCTTTTGAACAACGCAGCTGCCAT
  GATGCCTTGGGATGCCCTGGTCCTGGGGGACTCAGGTGCCTCCCTGATTCCTGTGGGAAC
  CCCGGGTTCAGGGCCAGGGCCTCCTTGGAATAAATGGTTATTGTTACTAGGTCCCCACCT
  TCCCTCTTTTCTGGAAGCCAAAGTCAGCCTCCCCAATAAAGTCCTCACTGCCTAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_138392
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138392.1, NP_612401.1
• RefSeq Size: 2362 bp
• RefSeq ORF: 2124 bp
• Locus ID: 92799
• UniProt ID: Q8TBC3
• Cytogenetics: 19q13.2
• Domains: BTB, WD40, K_tetra
• Protein Families: Ion Channels: Other
• Gene Summary: Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]