ASCL1 (NM_004316) Human Untagged Clone

CAT#: SC324383

ASCL1 (untagged)-Human achaete-scute complex homolog 1 (Drosophila) (ASCL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASCL1
• Synonyms: ASH1; bHLHa46; HASH1; MASH1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004316.2 CTTCTGGCCAGGGAACGTGGAAGGCGCACCGACAGGGATCCGGCCAGGGAGGGCGAGTGA
  AAGAAGGAAATCAGAAAGGAAGGGAGTTAACAAAATAATAAAAACAGCCTGAGCCACGGC
  TGGAGAGACCGAGACCCGGCGCAAGAGAGCGCAGCCTTAGTAGGAGAGGAACGCGAGACG
  CGGCAGAGCGCGTTCAGCACTGACTTTTGCTGCTGCTTCTGCTTTTTTTTTTCTTAGAAA
  CAAGAAGGCGCCAGCGGCAGCCTCACACGCGAGCGCCACGCGAGGCTCCCGAAGCCAACC
  CGCGAAGGGAGGAGGGGAGGGAGGAGGAGGCGGCGTGCAGGGAGGAGAAAAAGCATTTTC
  ACTTTTTTTGCTCCCACTCTAAGAAGTCTCCCGGGGATTTTGTATATATTTTTTAACTTC
  CGTCAGGGCTCCCGCTTCATATTTCCTTTTCTTTCCCTCTCTGTTCCTGCACCCAAGTTC
  TCTCTGTGTCCCCCTCGCGGGCCCCGCACCTCGCGTCCCGGATCGCTCTGATTCCGCGAC
  TCCTTGGCCGCCGCTGCGCATGGAAAGCTCTGCCAAGATGGAGAGCGGCGGCGCCGGCCA
  GCAGCCCCAGCCGCAGCCCCAGCAGCCCTTCCTGCCGCCCGCAGCCTGTTTCTTTGCCAC
  GGCCGCAGCCGCGGCGGCCGCAGCCGCCGCAGCGGCAGCGCAGAGCGCGCAGCAGCAGCA
  GCAGCAGCAGCAGCAGCAGCAGCAGGCGCCGCAGCTGAGACCGGCGGCCGACGGCCAGCC
  CTCAGGGGGCGGTCACAAGTCAGCGCCCAAGCAAGTCAAGCGACAGCGCTCGTCTTCGCC
  CGAACTGATGCGCTGCAAACGCCGGCTCAACTTCAGCGGCTTTGGCTACAGCCTGCCGCA
  GCAGCAGCCGGCCGCCGTGGCGCGCCGCAACGAGCGCGAGCGCAACCGCGTCAAGTTGGT
  CAACCTGGGCTTTGCCACCCTTCGGGAGCACGTCCCCAACGGCGCGGCCAACAAGAAGAT
  GAGTAAGGTGGAGACACTGCGCTCGGCGGTCGAGTACATCCGCGCGCTGCAGCAGCTGCT
  GGACGAGCATGACGCGGTGAGCGCCGCCTTCCAGGCAGGCGTCCTGTCGCCCACCATCTC
  CCCCAACTACTCCAACGACTTGAACTCCATGGCCGGCTCGCCGGTCTCATCCTACTCGTC
  GGACGAGGGCTCTTACGACCCGCTCAGCCCCGAGGAGCAGGAGCTTCTCGACTTCACCAA
  CTGGTTCTGAGGGGCTCGGCCTGGTCAGGCCCTGGTGCGAATGGACTTTGGAAGCAGGGT
  GATCGCACAACCTGCATCTTTAGTGCTTTCTTGTCAGTGGCGTTGGGAGGGGGAGAAAAG
  GAAAAGAAAAAAAAAGAAGAAGAAGAAGAAAAGAGAAGAAGAAAAAAACGAAAACAGTCA
  ACCAACCCCATCGCCAACTAAGCGAGGCATGCCTGAGAGACATGGCTTTCAGAAAACGGG
  AAGCGCTCAGAACAGTATCTTTGCACTCCAATCATTCACGGAGATATGAAGAGCAACTGG
  GACCTGAGTCAATGCGCAAAATGCAGCTTGTGTGCAAAAGCAGTGGGCTCCTGGCAGAAG
  GGAGCAGCACACGCGTTATAGTAACTCCCATCACCTCTAACACGCACAGCTGAAAGTTCT
  TGCTCGGGTCCCTTCACCTCCCCGCCCTTTCTTAGAGTGCAGTTCTTAGCCCTCTAGAAA
  CGAGTTGGTGTCTTTCGTCTCAGTAGCCCCCACCCCAATAAGCTGTAGACATTGGTTTAC
  AGTGAAACTATGCTATTCTCAGCCCTTTGAAACTCTGCTTCTCCTCCAGGGCCCGATTCC
  CAAACCCCATGGCTTCCCTCACACTGTCTTTTCTACCATTTTCATTATAGAATGCTTCCA
  ATCTTTTGTGAATTTTTTATTATAAAAAATCTATTTGTATCTATCCTAACCAGTTCGGGG
  ATATATTAAGATATTTTTGTACATAAGAGAGAAAGAGAGAGAAAAATTTATAGAAGTTTT
  GTACAAATGGTTTAAAATGTGTATATCTTGATACTTTAACATGTAATGCTATTACCTCTG
  CATATTTTAGATGTGTAGTTCACCTTACAACTGCAATTTTCCCTATGTGGTTTTGTAAAG
  AACTCTCCTCATAGGTGAGATCAAGAGGCCACCAGTTGTACTTCAGCACCAATGTGTCTT
  ACTTTATAGAAATGTTGTTAATGTATTAATGATGTTATTAAATACTGTTCAAGAAGAACA
  AAGTTTATGCAGCTACTGTCCAAACTCAAAGTGGCAGCCAGTTGGTTTTGATAGGTTGCC
  TTTTGGAGATTTCTATTACTGCCTTTTTTTTCTTACTGTTTTATTACAAACTTACAAAAA
  TATGTATAACCCTGTTTTATACAAACTAGTTTCGTAATAAAACTTTTTCCTTTTTTTAAA
  ATGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004316
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004316.2, NP_004307.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 711 bp
• Locus ID: 429
• UniProt ID: P50553
• Cytogenetics: 12q23.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]