GCAP1 (GUCA1A) (NM_000409) Human Untagged Clone

CAT#: SC324283

GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCAP1
• Synonyms: GUCA1A; GUCA1ANB-GUCA1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000409.2 CAGAAGTTCCACCTCACCATCAAGAAAGGCCCTGGTCCAGGATGTGCAAGGTGTGATGAA
  ATCACAGGGCTGGGGAAGGTCAGCTCGGGGTCACAAGAAGCCTGATGGGCAGGAAAGAGC
  ATGAAAGCCCCAGCCAGCCTCACCTGTGCGGCTGGGAGGACTCACAGAAACCCTCTGTAC
  CCAGTCATGGGCCAAAGACACCGTCATGCAAGGGGGTGAAGGCTCCACACTCGTCCCGGC
  CCCGGGCGTGGAAGCAGGACCTCGAGCAGTCTCTGGCAGCAGCCTATGTGCCGGTCGTTG
  TGGACTCTAAGGGGCAGAATCCGGACAAGCTCAGGGTACAGTCTCCCCTGGGGCTGCAAG
  GATTTAGTGGAGACTCTTAACACCAGTTCTCTGGCATCTGTGAGTTTGAGTGTGGGCCAT
  CATCTTCTTCCTTCTGCTCTCTCCCTCTCCACATTTCCCGGTACCATCTGATCCATCAGG
  CCCTTCTTTGCTCAGGCCTGAAGGACTCAGGCCTGTGAGAGAGGACGGCCCCGTTGTCGG
  CCAAGACACCTTTGGGCGAGGAGCAGCGAACAGGGCCTGTCCATCTCAGACGTCAGCCCC
  CTGAAGGCCTGAGCAATGGGCAACGTGATGGAGGGAAAGTCAGTGGAGGAGCTGAGCAGC
  ACCGAGTGCCACCAGTGGTACAAGAAGTTCATGACTGAGTGCCCCTCTGGCCAACTCACC
  CTCTATGAGTTCCGCCAGTTCTTCGGCCTCAAGAACCTGAGCCCGTCGGCCAGCCAGTAC
  GTGGAACAGATGTTTGAGACTTTTGACTTCAACAAGGACGGCTACATTGATTTCATGGAG
  TACGTGGCAGCGCTCAGCTTGGTCCTCAAGGGGAAGGTGGAACAGAAGCTCCGCTGGTAC
  TTCAAGCTCTATGATGTAGATGGCAACGGCTGCATTGACCGCGATGAGCTGCTCACCATC
  ATCCAGGCCATTCGCGCCATTAACCCCTGCAGCGATACCACCATGACTGCAGAGGAGTTC
  ACCGATACAGTGTTCTCCAAGATTGACGTCAACGGGGATGGGGAACTCTCCCTGGAAGAG
  TTTATAGAGGGCGTCCAGAAGGACCAGATGCTCCTGGACACACTGACACGAAGCCTGGAC
  CTTACCCGCATCGTGCGCAGGCTCCAGAATGGCGAGCAAGACGAGGAGGGGGCTGACGAG
  GCCGCTGAGGCAGCCGGCTGAGTGCACCGCCCGGCTGCTTCTGCACTAGCGGGTGGGGTG
  GTATGGTGGTGCCTGTTGGTGGTGTTCTTGTCTTAACCCTAGATAGAATCTAATGAACTC
  AGAGGCTTAGCTCGCCTCTTTAGGGTCCATGGTGGCAGCAGAGAGGCAGAAGTGGGAGTC
  CAGAGCCAGGAACAGTGAAGGATGGTTCCTGGCCCCTCTGAGTGACAGCTGGTGGCAGCA
  CTCCTTGCTGGGGGGCACTGTTCAACATCCCTCTGCCGTCGGGTGACCCCCTAGCCCTTC
  TGACTCCTCTCCCAGCTTTTCCCAGCTTTCCCCACTGAGCTTCTCCAGTCCATGCTCTTC
  TGGACGTGGACTCTCTGAGGCAGAACTGAGCTTTTCCAGGCCTCTTATGGAATCCTGCAG
  ATCCAGTGGCTGCAGCTTCAATCCCAGTGCTGCAATCACACATCCATTCTGCCCTGGGGG
  ACCCTGGAGCCTACTTGTGCGCTTTGCATTTCATTGATTGACGCCTCCCTTCAACAAGCA
  TTTACTGAGCGCCTACTATGTACTAATGCTAGATGTTAGATGTACAAAGAAGACAGTTTT
  CATCCTCTAGGAACTCATAGGCTAATGGTGAGACACACAGACAAACATCATTATAATAAA
  ATATGCTAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000409
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000409.2, NP_000400.2
• RefSeq Size: 1976 bp
• RefSeq ORF: 606 bp
• Locus ID: 2978
• UniProt ID: P43080
• Cytogenetics: 6p21.1
• Domains: EFh
• Protein Families: Druggable Genome
• Protein Pathways: Olfactory transduction
• Gene Summary: This gene encodes an enzyme that plays a role in the recovery of retinal photoreceptors from photobleaching. This enzyme promotes the activity of retinal guanylyl cyclase-1 (GC1) at low calcium concentrations and inhibits GC1 at high calcium concentrations. Mutations in this gene can cause cone dystrophy 3 and code-rod dystrophy 14. provided by RefSeq, Jul 2020]
  Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.