Cytochrome P450 Reductase (POR) (NM_000941) Human Untagged Clone

CAT#: SC324169

POR (untagged)-Human P450 (cytochrome) oxidoreductase (POR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 Reductase
• Synonyms: CPR; CYPOR; P450R
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000941.2 GGCTGCCAGCGTTTCATGATCAACATGGGAGACTCCCACGTGGACACCAGCTCCACCGTG
  TCCGAGGCGGTGGCCGAAGAAGTATCTCTTTTCAGCATGACGGACATGATTCTGTTTTCG
  CTCATCGTGGGTCTCCTAACCTACTGGTTCCTCTTCAGAAAGAAAAAAGAAGAAGTCCCC
  GAGTTCACCAAAATTCAGACATTGACCTCCTCTGTCAGAGAGAGCAGCTTTGTGGAAAAG
  ATGAAGAAAACGGGGAGGAACATCATCGTGTTCTACGGCTCCCAGACGGGGACTGCAGAG
  GAGTTTGCCAACCGCCTGTCCAAGGACGCCCACCGCTACGGGATGCGAGGCATGTCAGCG
  GACCCTGAGGAGTATGACCTGGCCGACCTGAGCAGCCTGCCAGAGATCGACAACGCCCTG
  GTGGTTTTCTGCATGGCCACCTACGGTGAGGGAGACCCCACCGACAATGCCCAGGACTTC
  TACGACTGGCTGCAGGAGACAGACGTGGATCTCTCTGGGGTCAAGTTCGCGGTGTTTGGT
  CTTGGGAACAAGACCTACGAGCACTTCAATGCCATGGGCAAGTACGTGGACAAGCGGCTG
  GAGCAGCTCGGCGCCCAGCGCATCTTTGAGCTGGGGTTGGGCGACGACGATGGGAACTTG
  GAGGAGGACTTCATCACCTGGCGAGAGCAGTTCTGGCTGGCCGTGTGTGAACACTTTGGG
  GTGGAAGCCACTGGCGAGGAGTCCAGCATTCGCCAGTACGAGCTTGTGGTCCACACCGAC
  ATAGATGCGGCCAAGGTGTACATGGGGGAGATGGGCCGGCTGAAGAGCTACGAGAACCAG
  AAGCCCCCCTTTGATGCCAAGAATCCGTTCCTGGCTGCAGTCACCACCAACCGGAAGCTG
  AACCAGGGAACCGAGCGCCACCTCATGCACCTGGAATTGGACATCTCGGACTCCAAAATC
  AGGTATGAATCTGGGGACCACGTGGCTGTGTACCCAGCCAACGACTCTGCTCTCGTCAAC
  CAGCTGGGCAAAATCCTGGGTGCCGACCTGGACGTCGTCATGTCCCTGAACAACCTGGAT
  GAGGAGTCCAACAAGAAGCACCCATTCCCGTGCCCTACGTCCTACCGCACGGCCCTCACC
  TACTACCTGGACATCACCAACCCGCCGCGTACCAACGTGCTGTACGAGCTGGCGCAGTAC
  GCCTCGGAGCCCTCGGAGCAGGAGCTGCTGCGCAAGATGGCCTCCTCCTCCGGCGAGGGC
  AAGGAGCTGTACCTGAGCTGGGTGGTGGAGGCCCGGAGGCACATCCTGGCCATCCTGCAG
  GACTGCCCGTCCCTGCGGCCCCCCATCGACCACCTGTGTGAGCTGCTGCCGCGCCTGCAG
  GCCCGCTACTACTCCATCGCCTCATCCTCCAAGGTCCACCCCAACTCTGTGCACATCTGT
  GCGGTGGTTGTGGAGTACGAGACCAAGGCCGGCCGCATCAACAAGGGCGTGGCCACCAAC
  TGGCTGCGGGCCAAGGAGCCTGTCGGGGAGAACGGCGGCCGTGCGCTGGTGCCCATGTTC
  GTGCGCAAGTCCCAGTTCCGCCTGCCCTTCAAGGCCACCACGCCTGTCATCATGGTGGGC
  CCCGGCACCGGGGTGGCACCCTTCATAGGCTTCATCCAGGAGCGGGCCTGGCTGCGACAG
  CAGGGCAAGGAGGTGGGGGAGACGCTGCTGTACTACGGCTGCCGCCGCTCGGATGAGGAC
  TACCTGTACCGGGAGGAGCTGGCGCAGTTCCACAGGGACGGTGCGCTCACCCAGCTCAAC
  GTGGCCTTCTCCCGGGAGCAGTCCCACAAGGTCTACGTCCAGCACCTGCTAAAGCAAGAC
  CGAGAGCACCTGTGGAAGTTGATCGAAGGCGGTGCCCACATCTACGTCTGTGGGGATGCA
  CGGAACATGGCCAGGGATGTGCAGAACACCTTCTACGACATCGTGGCTGAGCTCGGGGCC
  ATGGAGCACGCGCAGGCGGTGGACTACATCAAGAAACTGATGACCAAGGGCCGCTACTCC
  CTGGACGTGTGGAGCTAGGGGCCTGCCTGCCCCACCCACCCCACAGACTCCGGCCTGTAA
  TCAGCTCTCCTGGCTCCCTCCCGTAGTCTCCTGGGTGTGTTTGGCTTGGCCTTGGCATGG
  GCGCAGGCCCAGTGACAAAGACTCCTCTGGGCCTGGGGTGCATCCTCCTCAGCCCCCAGG
  CCAGGTGAGGTCCACCGGCCCCTGGCAGCACAGCCCAGGGCCTGCATGGGGGCACCGGGC
  TCCATGCCTCTGGAGGCCTCTGGCCCTCGGTGGCTGCACAGAAGGGCTCTTTCTCTCTGC
  TGAGCTGGGCCCAGCCCCTCCACATGATTTCCAGTGAGTGTAAATAATTTTAAATAACCT
  CTGGCCCTTGGAATAAAGTTCTGTTTTCTGTAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000941
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000941.2, NP_000932.3
• RefSeq Size: 2509 bp
• RefSeq ORF: 2043 bp
• Locus ID: 5447
• UniProt ID: P16435
• Cytogenetics: 7q11.23
• Domains: flavodoxin, NAD_binding_1, FAD_binding_1
• Protein Families: Druggable Genome, P450, Transmembrane
• Gene Summary: This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in higher primate species. This starting position is most commonly referred to in the literature, and the numbering system used to describe disease-associated mutations is based on this protein start. This includes data in the P450 oxidoreductase (POR) allele nomenclature locus-specific database, and the Human Gene Mutation Database (HGMD). This start codon is restricted to higher primate species, and it has a weak Kozak signal. However, it should be noted that an alternative downstream start codon, which has a strong Kozak signal and is much more widely conserved, is also present. The use of this downstream start codon would result in a protein that is 3 aa shorter at the N-terminus. Protein sequencing in PMID:2513880 indicates that this is the preferred start codon in vivo. It is therefore possible that the ribosome will initiate at the upstream start codon only a small fraction of the time (if at all), while leaky scanning will allow the downstream start codon to be used predominantly.