P5CS (ALDH18A1) (NM_002860) Human Untagged Clone

CAT#: SC323891

ALDH18A1 (untagged)-Human aldehyde dehydrogenase 18 family, member A1 (ALDH18A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P5CS
• Synonyms: ADCL3; ARCL3A; GSAS; P5CS; PYCS; SPG9; SPG9A; SPG9B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002860.3 TGCGGACCCGCGGTAGTGCCAGGGGGCGAAGGCGGCGGTGGTGAGGAAGATACTTTGGTT
  AGTGACCACATCGCAGCATGTTGAGTCAAGTTTACCGCTGTGGGTTCCAGCCCTTCAACC
  AACATCTTCTGCCCTGGGTCAAGTGTACAACCGTCTTCAGATCTCATTGTATCCAGCCTT
  CAGTCATCAGACATGTTCGTTCTTGGAGCAACATCCCGTTTATCACTGTACCCCTCAGTC
  GTACACATGGCAAGTCCTTCGCCCACCGCAGTGAGCTGAAGCATGCCAAGAGAATCGTGG
  TGAAGCTCGGCAGTGCCGTGGTGACCCGAGGGGATGAATGTGGCCTGGCCCTGGGGCGCT
  TGGCATCTATTGTTGAGCAGGTATCAGTGCTGCAGAATCAGGGCAGAGAGATGATGCTGG
  TGACCAGTGGAGCCGTAGCCTTTGGCAAACAACGCTTGCGCCATGAGATCCTTCTGTCTC
  AGAGCGTGCGGCAGGCCCTCCACTCGGGGCAGAACCAGCTGAAAGAAATGGCAATTCCAG
  TCTTAGAGGCACGAGCCTGTGCAGCTGCCGGACAGAGTGGGCTGATGGCCTTGTATGAGG
  CTATGTTTACCCAGTACAGCATCTGTGCTGCCCAGATTTTGGTGACCAATTTGGATTTCC
  ATGATGAGCAGAAGCGCCGGAACCTCAATGGAACACTTCATGAACTCCTTAGAATGAACA
  TTGTCCCCATTGTCAACACAAATGATGCTGTTGTCCCCCCAGCTGAGCCCAACAGTGACC
  TGCAGGGGGTAAATGTTATTAGTGTTAAAGATAATGATAGCCTGGCTGCCCGACTGGCTG
  TGGAAATGAAAACTGATCTCTTGATTGTTCTTTCAGATGTAGAAGGCCTTTTTGACAGCC
  CCCCAGGTTCAGATGATGCAAAGCTTATTGATATATTTTATCCCGGAGATCAGCAGTCTG
  TGACATTTGGAATCAAGTCTAGAGTGGGAATGGGTGGCATGGAAGCCAAGGTGAAAGCAG
  CCCTCTGGGCTTTGCAAGGTGGCACTTCTGTTGTTATTGCCAATGGAACCCACCCAAAGG
  TGTCTGGGCACGTCATCACAGACATTGTGGAGGGGAAGAAAGTTGGTACCTTCTTTTCAG
  AAGTAAAGCCTGCAGGCCCTACTGTTGAGCAGCAGGGAGAAATGGCGCGATCTGGAGGAA
  GGATGTTGGCCACCTTGGAACCTGAGCAGAGAGCAGAAATTATCCATCATCTGGCTGATC
  TGTTGACGGACCAGCGTGATGAGATCCTGTTAGCCAACAAAAAAGACTTGGAGGAGGCAG
  AGGGGAGACTTGCAGCTCCTCTGCTGAAACGTTTAAGCCTCTCCACATCCAAATTGAACA
  GCCTGGCCATCGGTCTGCGACAGATCGCAGCCTCCTCCCAGGACAGCGTGGGACGTGTTT
  TGCGCCGCACCCGAATCGCCAAAAACTTGGAACTGGAACAAGTGACTGTCCCAATTGGAG
  TTCTGCTGGTGATCTTTGAATCTCGTCCTGACTGTCTACCCCAGGTGGCAGCTTTGGCTA
  TCGCAAGTGGCAATGGCTTGTTACTCAAAGGAGGGAAGGAGGCTGCACACAGCAACCGGA
  TTCTCCACCTCCTGACCCAGGAGGCTCTCTCAATCCATGGAGTCAAGGAGGCCGTGCAAC
  TGGTGAATACCAGAGAAGAAGTTGAAGATCTTTGCCGCCTAGACAAAATGATAGATCTGA
  TCATTCCACGTGGCTCTTCCCAGCTGGTCAGAGACATCCAGAAAGCTGCTAAGGGGATTC
  CAGTGATGGGGCACAGCGAAGGGATCTGTCACATGTATGTGGATTCCGAGGCCAGTGTTG
  ATAAGGTCACCAGGCTAGTCAGAGACTCTAAATGTGAATATCCAGCTGCCTGTAATGCTT
  TGGAGACTTTGTTAATCCACCGGGATCTGCTCAGGACACCATTATTTGACCAGATCATTG
  ATATGCTGAGAGTGGAACAGGTAAAAATTCATGCAGGCCCCAAATTTGCCTCCTATCTGA
  CCTTCAGCCCCTCCGAAGTGAAGTCACTCCGAACTGAGTATGGGGACCTGGAATTATGCA
  TTGAAGTAGTGGACAACGTTCAGGATGCCATTGACCACATCCACAAGTATGGCAGCTCCC
  ACACGGATGTCATCGTCACAGAGGACGAAAACACAGCGGAGTTCTTCCTGCAGCACGTAG
  ACAGTGCCTGTGTGTTCTGGAATGCCAGCACTCGCTTTTCTGATGGTTACCGCTTTGGAC
  TGGGAGCTGAAGTGGGAATCAGTACATCGAGAATCCACGCCCGGGGACCAGTAGGACTTG
  AGGGACTGCTTACTACTAAGTGGCTGCTGCGAGGGAAGGACCACGTGGTCTCAGATTTCT
  CAGAGCATGGAAGTTTAAAATATCTTCATGAGAACCTCCCTATTCCTCAGAGAAACACCA
  ACTGAAAAGAGCCAGGAAAACCCGGGAATTTTCCAAAAGGTCTTCACGTTAAACTTGTCT
  TATCTCAGGAGAGAGCCCGCTCTTGTCTCCCAGTTCCTGGTAGGGTCTGCCTGTTGGAAA
  GTGTACCTGGATGCTTCTGGGCTCCGTTTGGCAATAGCAATCTTGGCTGATGTGCACAGT
  CTGGCTCCCAGCTCACCCTTTTTTTTTAAAGTAAGAAAATAGTTGCTACCGATAGGGACT
  TTGCCAAGTCCAATTATCTTCTAGGATTGAAAGGTGCATTTTCCCCATAAAAAAGGCGAG
  GAAAACCCATGGCTGCTTTGTGTCACCTCAGTGACTTACAGTCCCCCTTGGCATTTAGTT
  GGTACTAGAGCCAGTCATCCTTAACAAATCTTTTCACATTTTATTTCTTTCACATGTAGT
  CATCTTCAAAAAGGAAAGATTTGGAATTTTAGAAAAGGGGCAACTCTTCTTTTTAGCATT
  CTCATCAGAAAGTCACAAAAATCGATGGAATCATTTCCACTGGGAAGATTGACCTTTTGT
  ATTTATTTGTGGGGTAAATTAATAAGCATTCCAGATGCTTGCAGCTTCCTGCATCCAGGA
  GATGCTGTGTTCCCCGTGATGCAGCTGGAACCCAAGCTGCAGCAGGAGATGCAAGTTTCA
  GGATGTTCCCCACTGAGCTGGAGGAATATCTACAGCAGTGATGCTTGAAATTTTTGTATG
  AATTATTTTGTCGTCCTACCCTTTTCCTCCAAAACAAAAATTAGAGGATTATTTTAATAC
  TTTGGATTCTTCCCCCTTTTTTGAGAAATAAAGTTTTTTATGAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002860
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002860.3, NP_002851.2
• RefSeq Size: 3470 bp
• RefSeq ORF: 2388 bp
• Locus ID: 5832
• UniProt ID: P54886
• Cytogenetics: 10q24.1
• Domains: aakinase, aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1), also known as P5CSL, encodes the longest isoform (1). Variants 1, 4, and 5 all encode the same isoform (1).