Semaphorin 4D (SEMA4D) (NM_006378) Human Untagged Clone

CAT#: SC323843

SEMA4D (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Semaphorin 4D
• Synonyms: A8; BB18; C9orf164; CD100; coll-4; COLL4; GR3; M-sema-G; SEMAJ
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006378.2 GCTGTAACACTCACCGTGAAGGTCTGCAGCTTCACTCCCGAGCCAGCGAGACCACGAACC
  CACCAGAAGGAAGAAACTCTGAACACATCTGAACATCAGAAGGGACAGACTCCAGACGCG
  CCACCACTCTGCTAACACCAGATAGTGGAAAGAAACCATGTGCTGAAATGTTTGACGACA
  CTGATGGTTTGACTCTGCTAACTGGAATGGCTTATTGTGCAAGAAAGTACACCTGGTCGG
  GTCCTGGGGCTCATCTCTAGCACCAGCAAAGATTTCTGAAGACGTCTTTCTAGAAATGAC
  TGGAAAGTTTCAAGAGGCATAAGATACAGCATTTCTTCTGAGGCCCTGAAGAAGTATCAA
  GTGGGCTTTGACATTGCGGTGGTGAGAGCGACCCCTCCTCACCTGGAGAACTGGGAAATG
  TGGATTCTCAGGGACCGCGCTGTTCACGAGCTCCAGGCTGTGCTGCTGGCCCTGGTCCTG
  GGGCGCTGAGCCGCATCTGCAATAGCACACTTGCCCGGCCACCTGCTGCCGTGAGCCTTT
  GCTGCTGAAGCCCCTGGGGTCGCCTCTACCTGATGAGGATGTGCACCCCCATTAGGGGGC
  TGCTCATGGCCCTTGCAGTGATGTTTGGGACAGCGATGGCATTTGCACCCATACCCCGGA
  TCACCTGGGAGCACAGAGAGGTGCACCTGGTGCAGTTTCATGAGCCAGACATCTACAACT
  ACTCAGCCTTGCTGCTGAGCGAGGACAAGGACACCTTGTACATAGGTGCCCGGGAGGCGG
  TCTTCGCTGTGAACGCACTCAACATCTCCGAGAAGCAGCATGAGGTGTATTGGAAGGTCT
  CAGAAGACAAAAAAGCAAAATGTGCAGAAAAGGGGAAATCAAAACAGACAGAGTGCCTCA
  ACTACATCCGGGTGCTGCAGCCACTCAGCGCCACTTCCCTTTACGTGTGTGGGACCAACG
  CATTCCAGCCGGCCTGTGACCACCTGAACTTAACATCCTTTAAGTTTCTGGGGAAAAATG
  AAGATGGCAAAGGAAGATGTCCCTTTGACCCAGCACACAGCTACACATCCGTCATGGTTG
  ATGGAGAACTTTATTCGGGGACGTCGTATAATTTTTTGGGAAGTGAACCCATCATCTCCC
  GAAATTCTTCCCACAGTCCTCTGAGGACAGAATATGCAATCCCTTGGCTGAACGAGCCTA
  GTTTCGTGTTTGCTGACGTGATCCGAAAAAGCCCAGACAGCCCCGACGGCGAGGATGACA
  GGGTCTACTTCTTCTTCACGGAGGTGTCTGTGGAGTATGAGTTTGTGTTCAGGGTGCTGA
  TCCCACGGATAGCAAGAGTGTGCAAGGGGGACCAGGGCGGCCTGAGGACCTTGCAGAAGA
  AATGGACCTCCTTCCTGAAAGCCCGACTCATCTGCTCCCGGCCAGACAGCGGCTTGGTCT
  TCAATGTGCTGCGGGATGTCTTCGTGCTCAGGTCCCCGGGCCTGAAGGTGCCTGTGTTCT
  ATGCACTCTTCACCCCACAGCTGAACAACGTGGGGCTGTCGGCAGTGTGCGCCTACAACC
  TGTCCACAGCCGAGGAGGTCTTCTCCCACGGGAAGTACATGCAGAGCACCACAGTGGAGC
  AGTCCCACACCAAGTGGGTGCGCTATAATGGCCCGGTACCCAAGCCGCGGCCTGGAGCGT
  GCATCGACAGCGAGGCACGGGCCGCCAACTACACCAGCTCCTTGAATTTGCCAGACAAGA
  CGCTGCAGTTCGTTAAAGACCACCCTTTGATGGATGACTCGGTAACCCCAATAGACAACA
  GGCCCAGGTTAATCAAGAAAGATGTGAACTACACCCAGATCGTGGTGGACCGGACCCAGG
  CCCTGGATGGGACTGTCTATGATGTCATGTTTGTCAGCACAGACCGGGGAGCTCTGCACA
  AAGCCATCAGCCTCGAGCACGCTGTTCACATCATCGAGGAGACCCAGCTCTTCCAGGACT
  TTGAGCCAGTCCAGACCCTGCTGCTGTCTTCAAAGAAGGGCAACAGGTTTGTCTATGCTG
  GCTCTAACTCGGGCGTGGTCCAGGCCCCGCTGGCCTTCTGTGGGAAGCACGGCACCTGCG
  AGGACTGTGTGCTGGCGCGGGACCCCTACTGCGCCTGGAGCCCGCCCACAGCGACCTGCG
  TGGCTCTGCACCAGACCGAGAGCCCCAGCAGGGGTTTGATTCAGGAGATGAGCGGCGATG
  CTTCTGTGTGCCCGGATAAAAGTAAAGGAAGTTACCGGCAGCATTTTTTCAAGCACGGTG
  GCACAGCGGAACTGAAATGCTCCCAAAAATCCAACCTGGCCCGGGTCTTTTGGAAGTTCC
  AGAATGACGTGTTGAAGGCCGAGAGCCCCAAGTACGGTCTTATGGGCAGAAAAAACTTGC
  TCATCTTCAACTTGTCAGAAGGAGACAGTGGGGTGTACCAGTGCCTGTCAGAGGAGAGGG
  TTAAGAACAAAACGGTCTTCCAAGTGGTCGCCAAGCACGTCCTGGAAGTGAAGGTGGTTC
  CAAAGCCCGTAGTGGCCCCCACCTTGTCAGTTGTTCAGACAGAAGGTAGTAGGATTGCCA
  CCAAAGTGTTGGTGGCATCCACCCAAGGGTCTTCTCCCCCAACCCCAGCCGTGCAGGCCA
  CCTCCTCCGGGGCCATCACCCTTCCTCCCAAGCCTGCGCCCACCGGCACATCCTGCGAAC
  CAAAGATCGTCATCAACACGGTCCCCCAGCTCCACTCGGAGAAAACCATGTATCTTAAGT
  CCAGCGACAACCGCCTCCTCATGTCCCTCTTCCTCTTCTTCTTTGTTCTCTTCCTCTGCC
  TCTTTTTCTACAACTGCTATAAGGGATACCTGCCCAGACAGTGCTTGAAATTCCGCTCGG
  CCCTACTAATTGGGAAGAAGAAGCCCAAGTCAGATTTCTGTGACCGTGAGCAGAGCCTGA
  AGGAGACGTTAGTAGAGCCAGGGAGCTTCTCCCAGCAGAATGGGGAGCACCCCAAGCCAG
  CCCTGGACACCGGCTATGAGACCGAGCAAGACACCATCACCAGCAAAGTCCCCACGGATA
  GGGAGGACTCACAGAGGATCGACGACCTTTCTGCCAGGGACAAGCCCTTTGACGTCAAGT
  GTGAGCTGAAGTTCGCTGACTCAGACGCAGATGGAGACTGAGGCCGGCTGTGCATCCCCG
  CTGGTGCCTCGGCTGCGACGTGTCCAGGCGTGGAGAGTTTTGTGTTTCTCCTGTTCAGTA
  TCCGAGTCTCGTGCAGTGCTGCGTAGGTTAGCCCGCATCGTGCAGACAACCTCAGTCCTC
  TTGTCTATTTTCTCTTGGGTTGAGCCTGTGACTTGGTTTCTCTTTGTCCTTTTGGAAAAA
  TGACAATCCTTGCATCCCAGTCTTGTGTTCCGAAGTCAGTCGGAGTACTTGAAGAAGGCC
  CACGGGCGGCACGGAGTTCCTGAGCCCTTTCTGTAGTGGGGGAAAGGTGGCTGGACCTCT
  GTTGGCTGAGAAGAGCATCCCTTCAGCTTCCCCTCCCCGTAGCAGCCACTAAAAGATTAT
  TTAATTCCAGATTGGAAATGACATTTTAGTTTATCAGATTGGTAACTTATCGCCTGTTGT
  CCAGATTGGCACGAACCTTTTCTTCCACTTAATTATTTTTTTAGGATTTTGCTTTGATTG
  TGTTTATGTCATGGGTCATTTTTTTTTAGTTACAGAAGCAGATGTGTTAATATTTAGAAG
  AAGATGTATATCTTCCAGATTTTGTTATATATTTGGCATAAAATACGGCTTACGTTGCTT
  AAGATTCTCAGGGATAAACTTCCTTTTGCTAAATGCATTCTTTCTGCTTTTAGAAATGTA
  GACATAAACACTCCCCGGAGCCCACTCACCTTTTTTCTTTTTCTTTTTTTTTTTTTAACT
  TTATTCCTTGAGGGAAGCATTGTTTTTGGAGAGATTTTCTTTCTGTACTTCGTTTTACTT
  TTCTTTTTTTTTAACTTTTACTCTCTCGAAGAAGAGGACCTTCCCACATCCACGAGGTGG
  GTTTTGAGCAAGGGAAGGTAGCCTGGATGAGCTGAGTGGAGCCAGGCTGGCCCAGAGCTG
  AGATGGGAGTGCGGTACAATCTGGAGCCCACAGCTGTCGGTCAGAACCTCCTGTGAGACA
  GATGGAACCTTCACAAGGGCGCCTTTGGTTCTCTGAACATCTCCTTTCTCTTCTTGCTTC
  AATTGCATACCCACTGCCTGCCCAGACTTTCTATCCAGCCTCACTGAGCTGCCCACTACT
  GGAAGGGAACTGGGCCTCGGTGGCCGGGGCCGCGAGCTGTGACCACAGCACCCTCAAGCA
  TACGGCGCTGTTCCTGCCACTGTCCTGAAGATGTGAATGGGTGGTACGATTTCAACACTG
  GTTAATTTCACACTCCATCTCCCCGCTTTGTAAATACCCATCGGGAAGAGACTTTTTTTC
  CATGGTGAAGAGCAATAAACTCTGGATGTTTGTGCGCGTGTGTGGACAGTCTTATCTTCC
  AGCATGATAGGATTTGACCATTTTGGTGTAAACATTTGTGTTTTATAAGATTTACCTTGT
  TTTTATTTTTCTACTTTGAATTGTATACATTTGGAAAGTACCCAAATAAATGAGAAGCTT
  CTATCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006378
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006378.2, NP_006369.2
• RefSeq Size: 4675 bp
• RefSeq ORF: 2589 bp
• Locus ID: 10507
• UniProt ID: Q92854
• Cytogenetics: 9q22.2
• Domains: Sema, PSI, ig, IG, PSI
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).