hnRNP U (HNRNPU) (NM_004501) Human Untagged Clone

CAT#: SC323776

HNRNPU (untagged)-Human heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: hnRNP U
• Synonyms: DEE54; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1; HNRPU; pp120; SAF-A; SAFA; U21.1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004501.3 GGCGGATCCGCTCTGCAGCACGAACCCATCTCCAGCCGCAGCCGCAGCCGCCGCCCGGGC
  CGAGGAGCAGCCGCAGCAGCCGCCACCAGTGGCCGAGTGAGCGGAGCCGAGTTTGAGGCA
  GCGCCTAGCGGTGAATCGGGGCCCTCACCATGAGTTCCTCGCCTGTTAATGTAAAAAAGC
  TGAAGGTGTCGGAGCTGAAAGAGGAGCTCAAGAAGCGACGCCTTTCTGACAAGGGTCTCA
  AGGCCGAGCTCATGGAGCGACTCCAGGCTGCGCTGGACGACGAGGAGGCCGGGGGCCGCC
  CCGCCATGGAGCCCGGGAACGGCAGCCTAGACCTGGGCGGGGATTCCGCTGGGCGCTCGG
  GAGCAGGCCTCGAGCAGGAGGCCGCGGCCGGCGGCGATGAAGAGGAGGAGGAAGAGGAAG
  AGGAGGAGGAAGGAATCTCCGCTCTGGACGGCGACCAGATGGAGCTAGGAGAGGAGAACG
  GGGCCGCGGGGGCGGCCGACTCGGGCCCGATGGAGGAGGAGGAGGCCGCCTCGGAAGACG
  AGAACGGCGACGATCAGGGTTTCCAGGAAGGGGAAGATGAGCTCGGGGACGAAGAGGAAG
  GCGCGGGCGACGAGAACGGGCACGGGGAGCAGCAGCCTCAACCGCCGGCGACGCAGCAGC
  AACAGCCCCAACAGCAGCGCGGGGCCGCCAAGGAGGCCGCGGGGAAGAGCAGCGGCCCCA
  CCTCGCTGTTCGCGGTGACGGTGGCGCCGCCCGGGGCGAGGCAGGGCCAGCAGCAGGCGG
  GAGGGGACGGCAAAACAGAACAGAAAGGCGGAGATAAAAAGAGGGGTGTTAAAAGACCAC
  GAGAAGATCATGGCCGTGGATATTTTGAGTACATTGAAGAGAACAAGTATAGCAGAGCCA
  AATCTCCTCAGCCACCTGTTGAAGAAGAAGATGAACACTTCGATGACACAGTGGTTTGTC
  TTGATACTTATAATTGTGATCTACATTTTAAAATATCAAGAGATCGTCTCAGTGCTTCTT
  CCCTTACAATGGAGAGTTTTGCTTTTCTTTGGGCTGGAGGAAGAGCATCCTATGGTGTGT
  CAAAAGGCAAAGTGTGTTTTGAGATGAAGGTTACAGAGAAGATCCCAGTAAGGCATTTAT
  ATACAAAAGATATTGACATACATGAAGTTCGTATTGGCTGGTCACTAACTACAAGTGGAA
  TGTTACTTGGTGAAGAAGAATTTTCTTATGGGTATTCTCTAAAAGGAATAAAAACATGCA
  ACTGTGAGACTGAAGATTATGGAGAAAAGTTTGATGAAAATGATGTGATTACATGTTTTG
  CTAACTTTGAAAGTGATGAAGTAGAACTCTCGTATGCTAAGAATGGACAAGATCTTGGCG
  TTGCCTTCAAAATCAGTAAGGAAGTTCTTGCTGGACGGCCACTGTTCCCGCATGTTCTCT
  GCCACAACTGTGCAGTTGAATTTAATTTTGGTCAGAAGGAAAAGCCATATTTTCCAATAC
  CTGAAGAGTATACTTTCATCCAGAACGTCCCCTTAGAGGATCGAGTTAGAGGACCAAAGG
  GGCCTGAAGAGAAGAAAGATTGTGAAGTTGTGATGATGATTGGCTTGCCAGGAGCTGGAA
  AAACTACCTGGGTTACTAAACATGCAGCAGAAAATCCAGGGAAATATAACATTCTTGGCA
  CAAATACTATTATGGATAAGATGATGGTGGCAGGTTTTAAGAAGCAAATGGCAGATACTG
  GAAAACTGAACACACTGTTGCAGAGAGCCCCCCAGTGTCTTGGGAAATTTATTGAGATTG
  CTGCCCGAAAGAAGCGAAATTTTATTCTGGATCAGACAAATGTGTCTGCTGCTGCCCAGA
  GGAGAAAAATGTGCCTGTTTGCAGGCTTCCAGCGAAAAGCTGTTGTAGTTTGCCCAAAAG
  ATGAAGACTATAAGCAAAGAACACAGAAGAAAGCAGAAGTAGAGGGGAAAGACCTACCAG
  AACATGCGGTCCTCAAAATGAAAGGAAACTTTACCCTCCCAGAGGTAGCTGAGTGCTTTG
  ATGAAATAACCTATGTTGAACTTCAGAAGGAAGAAGCCCAAAAACTCTTGGAGCAATATA
  AGGAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACACTGGCTCAAAGAAAA
  GCAATAAAAATAAGAGTGGCAAGAACCAGTTTAACAGAGGTGGTGGCCATAGAGGACGTG
  GAGGATTCAATATGCGTGGTGGAAATTTCAGAGGAGGAGCCCCTGGGAATCGTGGCGGAT
  ATAATAGGAGGGGCAACATGCCACAGAGAGGTGGTGGCGGTGGAGGAAGTGGTGGAATCG
  GCTATCCATACCCTCGTGCCCCTGTTTTTCCTGGCCGTGGTAGTTACTCAAACAGAGGGA
  ACTACAACAGAGGTGGAATGCCCAACAGAGGGAACTACAACCAGAACTTCAGAGGACGAG
  GAAACAATCGTGGCTACAAAAATCAATCTCAGGGCTACAACCAGTGGCAGCAGGGTCAAT
  TCTGGGGTCAGAAGCCATGGAGTCAGCATTATCACCAAGGATATTATTGAATACCCAAAT
  AAAACGAACTGATACATATTTCTCCAAAACCTTCACAAGAAGTCGACTGTTTTCTTTAGT
  AGGCTAACTTTTTAAACATTCCACAAGAGGAAGTGCCTGCGGGTTCCTTTTTTAGAAGCT
  TTGTGGGTTGATTTTTTTTCTTTTCTTTTTTGTACATTTTTAATTGCAGTTTAAAAGTGA
  ATCGTAAGAGAACCTCAGCATTGTGCACGATAAGAGAATGTGTCAGTATTTCAGGGTTCT
  ACATTTTATCTGTAAAATGTGACTTTTTTTTTTTTTTATCACAACAGAAGTAAAATGTTG
  CTTTGTACCTGGTGTCTTTTATTAAGAATTTACTCCCCCCATTTCTCACAGAGAATAACA
  GTCGGGAGTCATTGTCACAATATAATAGAAATGTTAGCAACCAGATTCATGTAAGGACTA
  AGTGGTCCTCATGAATTGCATTAAGACTCTGTACTGCTCATATTACACTCCATCCTCTCT
  GTAGTTTGCTGGGTAGTGGAGGGGGTAAGCTAAATCATAGTTTCTGACAATAACTGGGAA
  GGTTTTTTCTTAAAATAACAATGGAATTGGTATAATTGGGATTGAAAACTAAAACTTGGA
  ACTAAGATAGAGAAGATGGAGTGTATGTAGAAGGGCTGTTAAAAATGTAAAACTTGGTTG
  CATTATTTGTGGAGGCTCAAACTTGTGAAGGTTAATACCATAATTTTTCCATTTGTTCTG
  CATTTTGATTCTGAAAAGAAAGCTGGCTTTGCCCATTTCTTATTAAAAAAACTTGTTGTA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004501
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004501.3, NP_004492.2
• RefSeq Size: 6789 bp
• RefSeq ORF: 2421 bp
• Locus ID: 3192
• UniProt ID: Q00839
• Cytogenetics: 1q44
• Domains: SAP, SPRY
• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14. [provided by RefSeq, Jun 2017]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region compared to variant 1, and encodes a shorter protein (isoform b).