CSK (NM_004383) Human Untagged Clone

CAT#: SC323685

CSK (untagged)-Kinase deficient mutant (K222M) of Human c-src tyrosine kinase (CSK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323685 sequence for NM_004383 edited (data generated by NextGen Sequencing)
  ATGTCAGCAATACAGGCCGCCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTC
  CACGGCACTGCCGAGCAGGACCTGCCCTTCTGCAAAGGAGACGTGCTCACCATTGTGGCC
  GTCACCAAGGACCCCAACTGGTACAAAGCCAAAAACAAGGTGGGCCGTGAGGGCATCATC
  CCAGCCAACTACGTCCAGAAGCGGGAGGGTGTGAAGGCGGGTACCAAACTCAGCCTCATG
  CCTTGGTTCCACGGCAAGATCACACGGGAGCAGGCTGAGCGGCTTCTGTACCCGCCGGAG
  ACAGGCCTGTTCCTGGTGCGGGAGAGCACCAACTACCCCGGAGACTACACGCTGTGCGTG
  AGCTGCGACGGCAAGGTGGAGCACTACCGCATCATGTACCATGCCAGCAAGCTCAGCATC
  GACGAGGAGGTGTACTTTGAGAACCTCATGCAGCTGGTGGAGCACTACACCTCAGACGCA
  GATGGACTCTGTACGCGCCTCATTAAACCAAAGGTCATGGAGGGCACAGTGGCGGCCCAG
  GATGAGTTCTACCGCAGCGGCTGGGCCCTGAACATGAAGGAGCTGAAGCTGCTGCAGACC
  ATCGGGAAGGGGGAGTTCGGAGACGTGATGCTGGGCGATTACCGAGGGAACAAAGTCGCC
  GTCATGTGCATTAAGAACGACGCCACTGCCCAGGCCTTCCTGGCTGAAGCCTCAGTCATG
  ACGCAACTGCGGCATAGCAACCTGGTGCAGCTCCTGGGCGTGATCGTGGAGGAGAAGGGC
  GGGCTCTACATCGTCACTGAGTACATGGCCAAGGGGAGCCTTGTGGACTACCTGCGGTCT
  AGGGGTCGGTCAGTGCTGGGCGGAGACTGTCTCCTCAAGTTCTCGCTAGATGTCTGCGAG
  GCCATGGAATACCTGGAGGGCAACAATTTCGTGCATCGAGACCTGGCTGCCCGCAATGTG
  CTGGTGTCTGAGGACAACGTGGCCAAGGTCAGCGACTTTGGTCTCACCAAGGAGGCGTCC
  AGCACCCAGGACACGGGCAAGCTGCCAGTCAAGTGGACAGCCCCTGAGGCCCTGAGAGAG
  AAGAAATTCTCCACTAAGTCTGACGTGTGGAGTTTCGGAATCCTTCTCTGGGAAATCTAC
  TCCTTTGGGCGAGTGCCTTATCCAAGAATTCCCCTGAAGGACGTCGTCCCTCGGGTGGAG
  AAGGGCTACAAGATGGATGCCCCCGACGGCTGCCCGCCCGCAGTCTATGAAGTCATGAAG
  AACTGCTGGCACCTGGACGCCGCCATGCGGCCCTCCTTCCTACAGCTCCGAGAGCAGCTT
  GAGCACATCAAAACCCACGAGCTGCACCTGTGA
  
  Clone variation with respect to NM_004383.2
  210 c=>t;665 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004383 unedited
  ACGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATT
  CGGCACGAGGGCGGCCCCGATCGAGCGTCCGGGGCGGCCCCCGGCAGCCAGCGCGACGTTCCAAAATCGA
  ACCTCAGTGGCGGCGCTCGGAAGCGGAACTCTGCCGGGGCCGCGCCGGCTACATTGTTTCCTCCCCCCGA
  CTCCCTCCCGCCCCCTTCCCCCGCCTTTCTTCCCTCCGCGACCCGGGCCGTGCGTCCGTCCCCCCTGCCT
  CTGCCTGGCGGGTCCCCTCCTCCCCCTCCTCCTTGCCACCCCATAACCTCTTTTGTTACCCGCACCCCCT
  GTGGGACCCCCTGGGCGCCCCTTCCCCTCCCCCCTTGGACCGCATTGGAACCCGTCCCGGCAGGCCGGTT
  GAAGGCCCCCCCGGGGGCAAAGTTGGCCCCGGACCCCGCAGTTGCCCCAGAAAAGGCTTTTATTGTTCCC
  AGTTTGAAAGGGTGGGGCTTTTACGGGACATTGGGGGGGACGCCAGAAAACCCTCCGGAAAAGAATGTGC
  CCAACACGGGCCCGCCGGGGCCATCCGTGCCCAAAAGTGTTTGCCAATTTAAATTTTACGGGACTGTCGA
  AAAACGAGACGTGCCTTTGCGAAGGAGAAATGTCTCACTTTTGGGGCCCGCCACGAGCCCCCCCTGGTGC
  AAAAGCACAACACGTGGGGGCCCGGAGGGGGGTCTTCCCCCCCCACTACTTCCTCAAACCCCGAGGTGTG
  TAAGACGCTATCACACATCATCCTCATTGCCGTGGTGTTCTCCGGCGGATATCTACCGGGACAGCGTGAT
  CAGCCTTTTTTACACCCGCAGAAAGCCTGTTCTCTGTGTGGTGGAAAACCACATACTACGAGAGCATACT
  AACCTGCGTCTTGAGCTGCTCACGCAAGGTGTACACATTCCAGCACTATGTAGATGCGCGCCAGG
  
  Kinase domain sequence

  >SC323685 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGCTGACTGACTGAAGGAGCTGAGCTGCTGCAGACCATCGGGAAGGGGGAGTTCGGAGACKTGATGCTGG
  GCGATTACCGAGGGAACAAAGTCGCCGTCATGTGCATTAAGAACGACGCCACTGCCCAGGCCTTCCTGGC
  TGAAGCCTCAGTCATGACGCAACTGCGGCATAGCAACCTGGTGCAGCTCCTGGGCGTGATCGTGGAGGAG
  AAGGGCGGGCTCTACATCGTCACTGAGTACATGGCCAAGGGGAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_004383
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004383.1, NP_004374.1
• RefSeq Size: 2420 bp
• RefSeq ORF: 1353 bp
• Locus ID: 1445
• UniProt ID: P41240
• Cytogenetics: 15q24.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.