Eph receptor A7 (EPHA7) (NM_004440) Human Untagged Clone

CAT#: SC323681

EPHA7 (untagged)-Kinase deficient mutant (K665M) of Human EPH receptor A7 (EPHA7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Eph receptor A7
• Synonyms: EHK-3; EHK3; EK11; HEK11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323681 sequence for NM_004440 edited (data generated by NextGen Sequencing)
  ATGGTTTTTCAAACTCGGTACCCTTCATGGATTATTTTATGCTACATCTGGCTGCTCCGC
  TTTGCACACACAGGGGAGGCGCAGGCTGCGAAGGAAGTACTACTGCTGGATTCTAAAGCA
  CAACAAACAGAGTTGGAGTGGATTTCCTCTCCACCCAATGGGTGGGAAGAAATTAGTGGT
  TTGGATGAGAACTATACACCGATACGAACATACCAGGTGTGCCAAGTCATGGAGCCCAAC
  CAAAACAACTGGCTGCGGACTAACTGGATTTCCAAAGGCAATGCACAAAGGATTTTTGTA
  GAATTGAAATTCACCCTGAGGGATTGTAACAGTCTTCCTGGAGTACTGGGAACTTGCAAG
  GAAACATTTAATTTGTACTATTATGAAACAGACTATGACACTGGCAGGAATATAAGAGAA
  AACCTCTATGTAAAAATAGACACCATTGCTGCAGATGAAAGTTTTACCCAAGGTGACCTT
  GGTGAAAGAAAGATGAAGCTTAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAG
  GGATTCTATCTTGCCTTTCAGGATGTAGGGGCTTGCATAGCTTTGGTTTCTGTCAAAGTG
  TACTACAAGAAGTGCTGGTCCATTATTGAGAACTTAGCTATCTTTCCAGATACAGTGACT
  GGTTCAGAATTTTCCTCTTTAGTCGAGGTTCGAGGGACATGTGTCAGCAGTGCAGAGGAA
  GAAGCGGAAAACGCCCCCAGGATGCACTGCAGTGCAGAAGGAGAATGGTTAGTGCCCATT
  GGAAAATGTATCTGCAAAGCAGGCTACCAGCAAAAAGGAGACACTTGTGAACCCTGTGGC
  CGTGGGTTCTACAAGTCTTCCTCTCAAGATCTTCAGTGCTCTCGTTGTCCAACTCACAGT
  TTTTCTGATAAAGAAGGCTCCTCCAGATGTGAATGTGAAGATGGGTATTACAGGGCTCCA
  TCTGACCCACCATACGTTGCATGCACAAGGCCTCCATCTGCACCACAGAACCTCATTTTC
  AACATCAACCAAACCACAGTAAGTTTGGAATGGAGTCCTCCTGCAGACAATGGGGGAAGA
  AACGATGTGACCTACAGAATATTGTGTAAGCGGTGCAGTTGGGAGCAGGGCGAATGTGTT
  CCCTGTGGGAGTAACATTGGATACATGCCCCAGCAGACTGGATTAGAGGATAACTATGTC
  ACTGTCATGGACCTGCTAGCCCACGCTAATTATACTTTTGAAGTTGAAGCTGTAAATGGA
  GTTTCTGACTTAAGCCGATCCCAGAGGCTCTTTGCTGCTGTCAGTATCACCACTGGTCAA
  GCAGCTCCCTCGCAAGTGAGCGGAGTAATGAAGGAGAGAGTACTGCAGCGGAGTGTCGAG
  CTTTCCTGGCAGGAACCAGAGCATCCCAATGGAGTCATCACAGAATATGAAATCAAGTAT
  TACGAGAAAGATCAAAGGGAACGGACCTACTCAACAGTAAAAACCAAGTCTACTTCAGCC
  TCCATTAATAATCTGAAACCAGGAACAGTGTATGTTTTCCAGATTCGGGCTTTTACTGCT
  GCTGGTTATGGAAATTACAGTCCCAGACTTGATGTTGCTACACTAGAGGAAGCTACAGGT
  AAAATGTTTGAAGCTACAGCTGTCTCCAGTGAACAGAATCCTGTTATTATCATTGCTGTG
  GTTGCTGTAGCTGGGACCATCATTTTGGTGTTCATGGTCTTTGGCTTCATCATTGGGAGA
  AGGCACTGTGGTTATAGCAAAGCTGACCAAGAAGGCGATGAAGAGCTTTACTTTCATTNN
  NNNNNNNNNNGCACCAAAACCTACATTGACCCTGAAACCTATGAGGACCCAAATAGAGCT
  GTCCATCAATTCGCCAAGGAGCTAGATGCCTCCTGTATTAAAATTGAGCGTGTGATTGGT
  GCAGGAGAATTCGGTGAAGTCTGCAGTGGCCGTTTGAAACTTCCAGGGAAAAGAGATGTT
  GCAGTAGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTG
  TGTGAAGCAAGCATCATGGGGCAGTTTGACCACCCAAATGTTGTCCATTTGGAAGGGGTT
  GTTACAAGAGGGAAACCAGTCATGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGAT
  GCATTTCTCAGGAAACATGATGGGCAATTTACAGTCATTCAGTTAGTAGGAATGCTGAGA
  GGAATTGCTGCTGGAATGAGATATTTGGCTGATATGGGATATGTTCACAGGGACCTTGCA
  GCTCGCAATATTCTTGTCAACAGCAATCTCGTTTGTAAAGTGTCAGATTTTGGCCTGTCC
  CGAGTTATAGAGGATGATCCAGAAGCTGTCTATACAACTACTGGTGGAAAAATTCCAGTA
  AGGTGGACAGCACCCGAAGCCATCCAGTACCGGAAATTCACATCAGCCAGTGATGTATGG
  AGCTATGGAATAGTCATGTGGGAAGTTATGTCTTATGGAGAAAGACCTTATTGGGACATG
  TCAAATCAAGATGTTATAAAAGCAATAGAAGAAGGTTATCGTTTACCAGCACCCATGGAC
  TGCCCAGCTGGCCTTCACCAGCTAATGTTGGATTGTTGGCAAAAGGAGCGTGCTGAAAGG
  CCAAAATTTGAACAGATAGTTGGAATTCTAGACAAAATGATTCGAAACCCAAATAGTCTG
  AAAACTCCCCTGGGAACTTGTAGTAGGCCAATAAGCCCTCTTCTGGATTAAAACACTCCT
  GATTTCACTACCTTTTGTTCAGTTGGAGAATGGCTACAAGCTATTAAGATGGAAAGATAT
  AAAGATAATTTCACGGCAGCTGGCTACAATTCCCTTGAATCAGTAGCCAGGATGACTATT
  GAGGATGTGATGAGTTTAGGGATCACACTGGTTGGTCATCAAAAGAAAATCATGAGCAGC
  ATTCAGACTATGAGAGCACAAATGCTACATTTACATGGAACTGGCATTCAAGTGTGA
  
  Clone variation with respect to NM_004440.3
  198 c=>a;981 g=>a;1341 t=>c;1799 t=>n;1800 t=>n;1801 a=>n;1802 a=>n;1803 a=>n;1804 t=>n;1805 t=>n;1806 t=>n;1807 c=>n;1808 c=>n;1809 a=>n;1810 g=>n;2076 g=>a;2749 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004440 unedited
  CCCGCCGTCTCAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAACACGGTGC
  GAGCGAACAGGAGTGGGGGGGAAATTAAAAAAAGCTAAACGTGGAGCAGCCGATCGGGGACCGAGAAGGG
  GAATCGATGCAAGGAGCACAATAAAACAAAAGCTACTTCGGAACAAACAGCATTTAAAAATCCACGACTC
  AAGATAACTGAAAACCTAAAAATAAAACCTGCTCATGCACCATGGTTTTTCAAACTCGGGTACCCTTCAT
  GGATTATTTTATGCTACATCTGGCTGCCTCCCGCTTTGCCCACACCCCAGGGGAAGCCCGCAAGGCTTGC
  CGAAGGAGGTAACTATACTGTGCTTGGATATTCTTAAAGCCACAACACAAACAGAGAGTTGGGAGGTGGG
  AATTCCCTCTCTCACACCCATGTGGGTGTGGGAAGAGAAATTAATTGGTTGGATTAGAAACACATATAAC
  CCGGATCAGAGAAATAACCAAGGGTGTGCACAGTCTCAGGGAGCCCCCACAAAAAAACTGGGCGGCGGGA
  CTATCTGGATTTTTCAAAGCGCAATGCACAAGAGATTTTGTGGAAAATGAAATTTTACCCGAGGGGGTGT
  ATCACACCTCCTCGTAGTGCAGTGGAAATGCGCGGAGACACATATATGGGTCTATGAAACCGACTATTAT
  GACTCGCGCGAGTATATAAGAAACATCTTGTGTAAAATAACACCTGTTGCGAGAAGATTTATCCCCGTGA
  CATGTGAGAGATAGACTACACCTAGTGAGAGAGATGAGCCTCTGTCAAGAGTCATCTGCTCTCAGTGAAG
  GGTGCAGCACTGTAGTGCAGGATCACCAAGTCGTCCTCTTGACATACCTCTTCGGACCTGACGCTGCAGA
  TCCTATTCCAGTCCTGGCAA
  
  Kinase domain sequence

  >SC323681 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CGKKCAWTGGTGCAGGAGATTCGGTGAGTCTGCAGTGGCCGTTTGAACTTCCAGGGAAAAGAGATGTTGC
  AGTAGCCATAATGACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTGTGTGAAGCAAGC
  ATCATGGGGCAGTTTGACCACCCAAATGTTGTCCATTTGGAAGGGGTTGTTACAAGAGGGAAACCAGTCA
  TGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGATGCATTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_004440
• Insert Size: 6600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004440.2, NP_004431.1
• RefSeq Size: 5229 bp
• RefSeq ORF: 2997 bp
• Locus ID: 2045
• UniProt ID: Q15375
• Cytogenetics: 6q16.1
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.