IRAKM (IRAK3) (NM_007199) Human Untagged Clone

CAT#: SC323662

IRAK3 (untagged)-Kinase deficient mutant (K192M) of Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRAKM
• Synonyms: ASRT5; IRAKM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323662 sequence for NM_007199 edited (data generated by NextGen Sequencing)
  ATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTG
  CCGCCCGCGCTGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGC
  TGGCGCGGCCTGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAG
  TATGTAGACCAAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAAC
  AAGACCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCAT
  TTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGA
  TTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCT
  GAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAA
  GGAACTAGAAATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATAC
  AGAGTGGAGATTCAAAACCTAACATATGCTGTCATGTTATTTAAACAGGAGAAAAAAATG
  CAGTGTAAGAAGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCAT
  CACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATT
  TATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCC
  CCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTAC
  CTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTT
  TTGGATGATCAGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCAC
  CTAGAACATCAGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTAC
  ATGCCAGAAGAGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTT
  GGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACAT
  ATCCAGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTC
  TCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGT
  TTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTA
  AATACTCTTGAAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTA
  AAGTCCTTCAGGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAA
  GATGATGAAAGCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGA
  ATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGAC
  AAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAA
  GAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAAT
  ATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGC
  TGTTCCTCCAAATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAA
  
  Clone variation with respect to NM_007199.2
  439 a=>g;575 a=>t;576 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_007199 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCCTGTCGCAGGC
  GTGCAGGGACCTGGACTCCGCCTCGTCCCCGGGGCTCGGGCAGCCGAGCCATGGCGGGGAACTGTGGGGC
  CCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTGCTCGGAGAGCTCTGCGCT
  GTTCTGGACAGCTGCGACGGGCGCGCTGGGCTGGGCGCGGCCTGGCAGAGAGAACTTTTCAAGCAGGCTG
  TGGCGGGGATGGTTCGTCCATATTGAAAAAGTATGGTAAGACACCAAGGGTTAAAGTGTGGACAAGAGAA
  AATAACCTTTGGTCCCTGGGGCCCAGAAAAAACAAGAACCATCGTTGAACCCTTTAACAGGGTCCCTCCC
  AGAAGAATGGACACATCGCCAAACTATTTTCTTTTATTTTCCAAACCAATGGGACCCTGGGTTGAATTCC
  TTTCAAAAAAAAATTTTTACGGGAGGGGGGGATTTCCCAAATATTTTTTTCAGGGGAACCCCCCAAGGGT
  CCCCGGGGGAAAAGTGTTTTATTTTCCGGCCCATAGGGAAGGGGGTCTGGGGTTTAACTTTTCCCCTCCC
  CTTTTTAAAAATTCTAAAAAGGGACCTAAAATTTTTCCACAAAAAAATCTCCAATTGGGGAAAAGGAAGA
  AATTTTTTTGGGGGTACCAAAGAGGGGAATATACAAACCCACCCCACATCTGGTCCCGGTGCGGTTTAAC
  ACACGGGAAGAAAATAGCATGTCGTTGTGAAGACACCTGTGGGAAGAGATGTGTTTCTCGGCATCGCATG
  TATTTTACGGATGTGCTGTACTCCCGCAACATCACTAGTGGTGTGCGTATATTATTCGACAGCAACGCA
  
  Kinase domain sequence

  >SC323662 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCATGMGCAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCCTGTCGCAGGCGTGCAGG
  GACCTGGACTCCGCCTCGTCCCCGGGGCTCGGGCAGCCGAGCCATGGCGGGGAACTGTGGGGCCCGCGGC
  GCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_007199
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007199.1, NP_009130.1
• RefSeq Size: 2288 bp
• RefSeq ORF: 1791 bp
• Locus ID: 11213
• UniProt ID: Q9Y616
• Cytogenetics: 12q14.3
• Domains: DEATH, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.