Insulin Receptor R (INSRR) (NM_014215) Human Untagged Clone

CAT#: SC323604

INSRR (untagged)-Kinase deficient mutant (K1013M) of Human insulin receptor-related receptor (INSRR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Insulin Receptor R
• Synonyms: IRR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014215, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGTGCCTAGTCTGTGGCCCTGGGGAGCATGCCTGCCTGTGATCTTCCTCTCCTTGGGATTTGGCC
  TGGATACAGTAGAGGTGTGCCCCAGCCTGGATATTCGCTCAGAGGTGGCAGAGCTTCGTCAGCTGGAGAA
  CTGCAGCGTGGTGGAGGGCCACCTGCAGATCCTGCTCATGTTCACAGCCACCGGGGAGGACTTCCGCGGC
  CTCAGCTTCCCTCGCCTCACCCAGGTCACCGACTACCTGCTGCTCTTCCGTGTCTACGGACTGGAGAGCC
  TGCGCGACCTCTTCCCCAACCTAGCAGTCATCCGCGGGACGCGCCTCTTCCTGGGCTATGCACTGGTCAT
  CTTTGAGATGCCACATCTGCGTGACGTGGCACTGCCTGCACTTGGGGCCGTGCTGCGTGGGGCTGTGCGT
  GTGGAGAAGAACCAGGAGCTCTGCCACCTCTCCACCATTGACTGGGGACTGCTGCAGCCAGCACCTGGCG
  CCAACCACATCGTGGGCAACAAGCTGGGCGAGGAGTGTGCTGACGTGTGCCCTGGTGTGCTGGGTGCTGC
  TGGTGAGCCCTGTGCCAAGACCACCTTCAGCGGGCACACTGACTACAGATGCTGGACCTCCAGCCACTGC
  CAGAGAGTGTGCCCCTGCCCCCATGGGATGGCTTGCACAGCGAGGGGCGAGTGCTGCCACACCGAATGCC
  TGGGGGGCTGCAGCCAGCCAGAAGACCCTCGTGCCTGTGTAGCTTGCCGCCACCTCTACTTCCAGGGTGC
  CTGCCTGTGGGCCTGCCCGCCAGGCACCTACCAGTATGAGTCCTGGCGCTGTGTCACAGCTGAGCGCTGT
  GCCAGCCTGCACTCTGTGCCCGGCCGTGCCTCCACCTTCGGCATACACCAGGGCAGTTGCCTGGCCCAGT
  GCCCTTCTGGCTTCACCCGTAATAGCAGCAGCATATTCTGCCACAAGTGCGAGGGGCTGTGCCCTAAAGA
  GTGCAAGGTAGGCACCAAGACCATCGACTCCATCCAGGCGGCACAGGATCTTGTGGGCTGCACGCATGTG
  GAGGGAAGCCTCATCCTCAACCTTCGCCAGGGCTACAACCTGGAGCCACAGCTGCAGCACAGCCTGGGGC
  TGGTAGAAACCATTACTGGCTTCCTCAAAATCAAGCACTCCTTTGCCCTCGTGTCCCTGGGCTTTTTCAA
  GAACCTCAAACTAATCCGGGGAGACGCCATGGTGGATGGGAACTACACTCTCTACGTGCTGGACAACCAG
  AACCTACAACAGCTAGGGTCCTGGGTGGCCGCGGGGCTCACCATTCCCGTGGGCAAGATCTACTTCGCCT
  TCAACCCGCGCCTCTGCTTGGAACACATCTACCGACTGGAGGAGGTGACAGGCACGCGAGGTCGGCAGAA
  CAAGGCTGAGATCAACCCCCGCACCAACGGAGACCGCGCCGCCTGCCAGACTCGCACCCTGCGCTTCGTG
  TCCAACGTGACGGAGGCAGACCGCATCCTGCTACGCTGGGAGCGCTATGAGCCACTGGAGGCCCGCGACC
  TGCTCAGCTTCATCGTGTACTACAAGGAGTCCCCATTCCAGAACGCCACAGAGCACGTGGGTCCAGATGC
  TTGTGGAACCCAGAGCTGGAACCTGCTGGATGTGGAGCTGCCCCTAAGCCGCACCCAGGAGCCAGGGGTG
  ACCCTAGCCTCCCTCAAGCCTTGGACACAGTACGCAGTGTTTGTGCGGGCCATCACGCTAACCACTGAGG
  AGGACAGCCCTCATCAAGGAGCCCAGAGTCCCATCGTCTACCTCCGAACGCTGCCTGCAGCTCCCACGGT
  GCCCCAAGACGTCATCTCCACGTCCAACTCCTCCTCCCACCTCCTGGTGCGCTGGAAGCCACCGACCCAG
  CGCAATGGGAACCTCACCTACTACCTGGTGCTGTGGCAGCGGCTGGCAGAGGACGGCGACCTCTACCTCA
  ATGACTACTGCCACCGCGGCTTGCGGCTGCCCACCAGCAACAACGATCCGCGCTTCGACGGCGAAGACGG
  GGATCCTGAGGCCGAGATGGAGTCCGACTGCTGCCCTTGCCAGCACCCACCTCCTGGTCAGGTTCTGCCC
  CCGCTGGAGGCGCAAGAGGCCTCGTTCCAGAAGAAGTTTGAAAACTTTCTACACAACGCGATCACCATCC
  CCATATCCCCTTGGAAGGTGACGTCCATCAACAAGAGCCCCCAAAGGGACTCAGGGCGGCACCGCCGGGC
  AGCTGGGCCCCTCCGGCTGGGGGGCAACAGCTCGGATTTCGAGATCCAGGAGGACAAGGTGCCCCGTGAG
  CGAGCGGTGCTGAGCGGCCTGCGCCACTTCACGGAATACCGGATCGACATCCATGCCTGCAACCACGCGG
  CGCACACCGTGGGCTGCAGCGCCGCCACCTTCGTCTTTGCGCGCACCATGCCCCACAGAGAGGCTGATGG
  TATTCCAGGAAAGGTGGCCTGGGAGGCCTCCAGCAAGAACAGTGTCCTTCTGCGCTGGCTCGAGCCACCA
  GACCCCAACGGACTCATCCTCAAGTACGAAATCAAGTACCGCCGCTTGGGAGAGGAGGCCACAGTGCTGT
  GTGTGTCCCGTCTTCGATATGCGAAGTTTGGGGGAGTCCACCTGGCCCTGCTGCCCCCTGGAAACTACTC
  TGCCAGGGTTAGGGCAACCTCACTGGCTGGCAATGGCTCTTGGACAGACAGTGTTGCCTTCTACATCCTT
  GGCCCAGAGGAGGAGGATGCTGGGGGGCTGCATGTCCTCCTCACTGCCACCCCTGTGGGGCTCACGCTGC
  TCATCGTTCTTGCTGCCCTTGGTTTCTTCTACGGCAAGAAGAGAAACAGAACCCTGTATGCTTCTGTGAA
  TCCAGAGTACTTCAGCGCCTCTGATATGTATGTCCCTGATGAATGGGAGGTGCCTCGGGAGCAGATCTCG
  ATAATCCGGGAACTGGGCCAGGGCTCTTTTGGGATGGTATATGAGGGGCTGGCACGAGGACTTGAGGCTG
  GAGAGGAGTCCACACCCGTGGCCCTGAAGACGGTGAATGAGCTGGCCAGCCCACGGGAATGCATTGAGTT
  CCTCAAGGAAGCTTCTGTCATGAAAGCCTTCAAGTGTCACCATGTGGTGCGTCTCCTGGGTGTGGTATCT
  CAGGGCCAGCCAACTCTGGTCATCATGGAGTTAATGACCCGTGGGGACCTCAAGAGCCATCTTCGATCTT
  TGCGGCCTGAGGCAGAGAACAACCCTGGGCTCCCACAGCCAGCATTGGGGGAAATGATCCAAATGGCTGG
  TGAGATTGCAGACGGCATGGCCTACCTTGCTGCCAACAAGTTTGTGCACCGAGATCTAGCAGCCCGCAAC
  TGCATGGTGTCCCAGGACTTCACCGTCAAGATCGGGGACTTCGGGATGACTCGGGACGTGTATGAGACAG
  ACTATTACCGCAAGGGTGGGAAGGGGCTGCTGCCCGTGCGCTGGATGGCCCCCGAGTCCCTCAAAGATGG
  GATCTTCACCACCCACTCGGATGTCTGGTCCTTTGGCGTGGTACTCTGGGAGATTGTGACCCTGGCAGAA
  CAACCCTACCAGGGCCTGTCCAATGAGCAGGTGCTGAAGTTCGTCATGGATGGCGGGGTCCTGGAGGAGC
  TGGAGGGCTGTCCCCTTCAGCTGCAGGAGCTGATGAGCCGCTGCTGGCAGCCGAACCCACGCCTGCGCCC
  ATCTTTCACACACATTCTGGACAGCATACAGGAGGAGCTGCGGCCCTCCTTCCGCCTCCTCTCCTTCTAC
  TACAGCCCGGAATGCCGGGGGGCCCGGGGCTCCCTGCCTACCACCGATGCAGAGCCTGACTCCTCACCCA
  CTCCAAGAGACTGCAGCCCTCAAAATGGGGGTCCAGGGCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014215 unedited
  CTCCCCGCTCGTTGCAGCAGATGGGCGGTAGGCGTGTACGGAAGGGAGGTCTATATAAGCAGAGCTCGTT
  TAGTGAACCGTCATTAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCACTGGCCGTCCTTCT
  ACAACGTCTTTACTGGGAAAACCCTGGCGTGAATTCGGCACGAGGTCATCTTTGAGATGCCACATCTGCG
  TGACGTGGCACTGCCTGCACTTGGGGCCGTGCTGCGTGGGCTGTGCGTGTGGAGAAGAACCAGAGCCCTG
  CCACCTCTCACCATTGACCGGGGACTCCTGCAACACCACCTGGCGCCACCAATCTGGGCACAATCTGGTT
  TAAGATTGCTGACTTCTGCCTGTTGTGCTGGTGCTGAGGTGCCCCGTGCCAAACCACCTAACGGCCCCTT
  TCTTCTGATCTTCCCCCTCAACCCTGCAAAAAAGGCCCCCCTCCCAAGGAAAGCCTTGGCCACAAGTGCC
  ATGCCCCCACCCCAAGGCCCGGGGGCTCAGAACCCGAAAAACTTGGCCGTTTTACCTTGCCCCCCCCAAC
  TTCCGGGGGCGCCGGGGCCCTCCCCAAACTTTCCATTAAACCCTGCAATAGCCAATAAAACTTGGGAAAA
  TGAAATGGGCTGGCAGCCTCATAAATG
  
  Kinase domain sequence

  >SC323604 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GGMGATCTCGWATCCGGGACTGGGCCAGGGCTCTTTTGGGATGGTATATGAGGGGCTGGCACGAGGACTT
  GAGGCTGGAGAGGAGTCCACACCCGTGGCCCTGATGACGGTGAATGAGCTGGCCAGCCCACGGGAATGCA
  TTGAGTTCCTCAAGGAAGCTTCTGTCATGAAAGCCTTCAAGTGTCACCATGTGGTGCGTCTCCTGGGTGT
  GGTATCTCAGGGCCAGCCAACTCTGGTCATCATGGAGTTAATGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_014215
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014215.1, NP_055030.1
• RefSeq Size: 4193 bp
• RefSeq ORF: 3894 bp
• Locus ID: 3645
• UniProt ID: P14616
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Prostate cancer, Regulation of actin cytoskeleton
• Gene Summary: Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.[UniProtKB/Swiss-Prot Function]