STK33 (NM_030906) Human Untagged Clone

CAT#: SC323587

STK33 (untagged)-Kinase deficient mutant (K145M) of Human serine/threonine kinase 33 (STK33)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK33
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030906, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGATAGTGGCTTAGATAAAAAATCCACAAAATGCCCCGACTGTTCATCTGCTTCTCAGAAAGATG
  TACTTTGTGTATGTTCCAGCAAAACAAGGGTTCCTCCAGTTTTGGTGGTGGAAATGTCACAGACATCAAG
  CATTGGTAGTGCAGAATCTTTAATTTCACTGGAGAGAAAAAAAGAAAAAAATATCAACAGAGATATAACC
  TCCAGGAAAGATTTGCCCTCAAGAACCTCAAATGTAGAGAGAAAAGCATCTCAGCAACAATGGGGTCGGG
  GCAACTTTACAGAAGGAAAAGTTCCTCACATAAGGATTGAGAATGGAGCTGCTATTGAGGAAATCTATAC
  CTTTGGAAGAATATTGGGAAAAGGGAGCTTTGGAATAGTCATTGAAGCGACAGACAAGGAAACAGAAACG
  AAGTGGGCAATTAAAAAAGTGAACAAAGAAAAGGCTGGAAGCTCTGCTGTGAAGTTACTTGAACGAGAGG
  TGAACATTCTGAAAAGTGTAAAACATGAACACATCATACATCTGGAACAAGTATTTGAAACGCCAAAGAA
  AATGTACCTTGTGATGGAGCTTTGTGAGGATGGAGAACTCAAAGAAATTCTGGATAGGAAAGGGCATTTC
  TCAGAGAATGAGACAAGGTGGATCATTCAAAGTCTCGCATCAGCTATAGCATATCTTCACAATAATGATA
  TTGTACATAGAGATCTGAAACTGGAAAATATAATGGTTAAAAGCAGTCTTATTGATGATAACAATGAAAT
  AAACTTAAACATAAAGGTGACTGATTTTGGCTTAGCGGTGAAGAAGCAAAGTAGGAGTGAAGCCATGCTG
  CAGGCCACATGTGGGACTCCTATCTATATGGCCCCTGAAGTTATCAGTGCCCACGACTATAGCCAGCAGT
  GTGACATTTGGAGCATAGGCGTCGTAATGTACATGTTATTACGTGGAGAACCACCCTTTTTGGCAAGCTC
  AGAAGAGAAGCTTTTTGAGTTAATAAGAAAAGGAGAACTACATTTTGAAAATGCAGTCTGGAATTCCATA
  AGTGACTGTGCTAAAAGTGTTTTGAAACAACTTATGAAAGTAGATCCTGCTCACAGAATCACAGCTAAGG
  AACTACTAGATAACCAGTGGTTAACAGGCAATAAACTTTCTTCGGTGAGACCAACCAATGTATTAGAGAT
  GATGAAGGAATGGAAAAATAACCCAGAAAGTGTTGAGGAAAACACAACAGAAGAGAAGAATAAGCCGTCC
  ACTGAAGAAAAGTTGAAAAGTTACCAACCCTGGGGAAATGTCCCTGATGCCAATTACACTTCAGATGAAG
  AGGAGGAAAAACAGTCTACTGCTTATGAAAAGCAATTTCCTGCAACCAGTAAGGACAACTTTGATATGTG
  CAGTTCAAGTTTCACATCTAGCAAACTCCTTCCAGCTGAAATCAAGGGAGAAATGGAGAAAACCCCTGTG
  ACTCCAAGCCAAGGAACAGCAACCAAGTACCCTGCTAAATCCGGCGCCCTGTCCAGAACCAAAAAGAAAC
  TCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_030906 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAAGGAGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCCCGTCCAC
  TGCCCTGCGGTTGCTCTGCGGGCTGAAAAGTTTCTCCCGGTGCAGAATTCCGGGCTCAGCGACAGCCTGC
  GCCGAGTGTGCGCACCTGTCGGAGACCCGCCAGTCCGCCGGCCGCGGCCTGAAGTTAAATCATTTTGGAA
  AGTGATACAGCTCTCACGTGTGAATATGTGTCTAGTGCATCCTTTAACCTGAGGACTTCACCCAGTTCGA
  AATTACAGTTTTCACCCATCAACTACCCTTATCCTTTTTGGCCTGTTTTTCTTCCTCAACAGTGGAAAAC
  ATTTTTAAAGTTGCTTTTGTTTGCAAAGTTAACCAAAGGGTGGATATTGGCTTAAATAAAAATTCCCAAA
  AATGCCCCGACTGTTCATCTGCTTTTAGAAAAAATGTACTTTTGTTTATTTTCCCCCAAAACAAGGGTTC
  CTTCCAGTTTTGGGTGGGGGGAAATGTACCAGAACATCAGCCATTGGTAAGGGCAAAACCTTTTATTTTC
  CCGGGAGGAAAAAAGAGAAAAATATTCCACAAAATTTTACCCTCCGAGAAAAAATTGCCCCCTGAAAACC
  CCATGTTGAGAGAAAAACCTCTCCCCACCACCTGTGGGGCCGCGGGAGCAACTTCACAAAAGGAAAATCT
  CCCCACATGGGTGTTGAAGAAGGAGGCTCTCTTTGGAAAAACTCACTCCTTGAGAAATAAATTTTGAAAA
  GAGGGCTTCTTGGAAATACTCTATGCATCACCACAGAGAGACCACAACAAACCAGGTGCGCATTATAGTA
  GATGCACACAAAGAGCGGCTGTCACCCCCTGCGCGGTAATCTGACGCAGCAGAGAGGATCCTACATGAAT
  GTAGCATGCTATCACCTATCTGCTGTAGCAGGCATTGATGTGCTA
  
  Kinase domain sequence

  >SC323587 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TTRCTGCTATTGAGGAATCTATACCTTTGGAAGATATTGGGAAAAGGGAGCTTTGGAATAGTCATTGAAG
  CTACAGACAAGGAAACAGAAACGAAGTGGGCAATTATGAAAGTGAACAAAGAAAAGGCTGGAAGCTCTGC
  TGTGAAGTTACTTGAACGAGAGGTGAACATTCTGAAAAGTGTAAAACATGAACACATCATACATCTGGAA
  CAAGTATTTGAAACGCCAAAGAAAATGTACCTTGTGATGGAGCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_030906
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030906.2, NP_112168.1
• RefSeq Size: 2707 bp
• RefSeq ORF: 1545 bp
• Locus ID: 65975
• UniProt ID: Q9BYT3
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 4-11 all encode the same isoform (a).