PAK6 (NM_020168) Human Untagged Clone

CAT#: SC323578

PAK6 (untagged)-Kinase deficient mutant (K436M) of Human p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK6
• Synonyms: PAK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020168, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCACCGTGTCCACA
  CCTCCTTCGACCCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACT
  GCGGCGCCCCAAGCCCGTGGTGGACCCTTCGCGAATCACACGGGTGCAGCTCCAGCCCATGAAGACAGTG
  GTGCGGGGCAGCGCGATGCCTGTGGATGGCTACATCTCGGGGCTGCTCAACGACATCCAGAAGTTGTCAG
  TCATCAGCTCCAACACCCTGCGTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT
  GGGGGATGAGCACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCAGTCTGAGCGCACTGACCCC
  CACGGCCTCTACCTCAGCTGCAACGGGGGCACACCAGCAGGCCACAAGCAGATGCCGTGGCCCGAGCCAC
  AGAGCCCACGGGTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTGGGCCCCGCCGAGTTTCAGGG
  TGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAGAGCTCCCCACCAGGAGCCTCGCCCCCCACG
  GGCACCAATAGGCATGGAATGAAGGCTGCCAAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGG
  TGGGCTCAGCCACAGGCAGGCCAGGTGGGGAAGGCAGCCCTAGCCCTAAGACCCGGGAGAGCAGCCTGAA
  GCGCAGGCTATTCCGAAGCATGTTCCTGTCCACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGC
  CCTCCACCACAGAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCAAGCCTGGTGG
  CCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACCTTCAGCCCTCTGACCACTTCGGATACCAG
  CAGCCCCCAGAAGTCCCTCCGCACAGCCCCGGCCACAGGCCAGCTTCCAGGCCGGTCTTCCCCAGCGGGA
  TCCCCCCGCACCTGGCACGCCCAGATCAGCACCAGCAACCTGTACCTGCCCCAGGACCCCACGGTTGCCA
  AGGGTGCCCTGGCTGGTGAGGACACAGGTGTTGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGT
  GGTGGACCAGGGTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATC
  GTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCAAGATGATGGACCTCAGGAAGCAGC
  AGCGCAGGGAGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGAT
  GTACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCTGCAGGGAGGAGCCCTCACA
  GACATCGTCTCCCAAGTCAGGCTGAATGAGGAGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCC
  TGGCCTACCTGCATGCTCAGGGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCTGACCCTCGA
  TGGCAGGGTGAAGCTCTCGGACTTCGGATTCTGTGCTCAGATCAGCAAAGACGTCCCTAAGAGGAAGTCC
  CTGGTGGGAACCCCCTACTGGATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACTGAGGTGGATA
  TCTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCGTACTTCAGTGACTCCCCAGT
  GCAAGCCATGAAGAGGCTCCGGGACAGCCCCCCACCCAAGCTGAAAAACTCTCACAAGGTCTCCCCAGTG
  CTGCGAGACTTCCTGGAGCGGATGCTGGTGCGGGACCCCCAAGAGAGAGCCACAGCCCAGGAGCTCCTAG
  ACCACCCCTTCCTGCTGCAGACAGGGCTACCTGAGTGCCTGGTGCCCCTGATCCAGCTCTACCGAAAGCA
  GACCTCCACCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020168 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCATCTGATTATTT
  TGGGAGCTGGAAACTTGGAGCTGCACCTGAGTCCCGCCCCTTCTAGCTCTCCCCTCCCTACCTTGGGCTC
  CAGGAAGATGGGACTTGCTGTGAGTCTGCTGCCACCCCCTAAAGATATGGAAGACGCTGTGGGGGCCAGA
  AGTGCCCGGGGGGCTGTGGCAGCAGGCAGAGTGCAATAGCAGATATGGTGGTCAGGCTGCCCGTGTGTGT
  CCTCTGGAGGTGTTGGGACAGAAGGGCAGTCTTGGTCCGAGCTGACTGGGAGTCCTCCCGGGCTGGCTCT
  GACCTCATCCTCCCACGGGGATTGTTTCGGTAAAGGAGTGGCTTCTGGGGGTCGGGAGTGGCATTTTGGA
  GGAGCGAGGCTTGATTTGCTTTAGGCTGGGCCTGGGCAGGAGTGGCCGCTTCCTGGGCTAAGAGACAGCC
  ACCAGCCTGCATGGGAAAACGCAGGACCCCGCTGCCCAAAGAACAGCCACGGCCTGGAGAGACTGCCCAC
  ACAGGTCCAGGTCTCCTCTCTACCCCTAAGAGAAGCCATTCGGTAAGATTCCAGAAAGCGAAGCCCGAAG
  CCCTGTCCGAAAAGAAACGCTGATTCAGCCAAAATCGAACGTGACACTCTGGATGAGAGAGTGTGACTCC
  CATTTGCAACTGGAACTAGGCGACTGGACCTGGGATCCAGTACTCCTGACATGCGCGACAGGCGGGGGAA
  T
  
  Kinase domain sequence

  >SC323578 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GKCATGCKACCCGGCTGCTGCTGGAAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATCGTCTGCTTG
  GCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCATGATGATGGACCTCAGGAAGCAGCAGCGCAGGG
  AGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGATGTACAAGAG
  CTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCTGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_020168
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020168.3, NP_064553.1
• RefSeq Size: 3889 bp
• RefSeq ORF: 2046 bp
• Locus ID: 56924
• UniProt ID: Q9NQU5
• Cytogenetics: 15q15.1
• Domains: PBD, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: This gene encodes a member of a family of p21-stimulated serine/threonine protein kinases, which contain an amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. These kinases function in a number of cellular processes, including cytoskeleton rearrangement, apoptosis, and the mitogen-activated protein (MAP) kinase signaling pathway. The protein encoded by this gene interacts with androgen receptor (AR) and translocates to the nucleus, where it is involved in transcriptional regulation. Changes in expression of this gene have been linked to prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 4 encode the same isoform (1).