JNK2 (MAPK9) (NM_002752) Human Untagged Clone

CAT#: SC323575

MAPK9 (untagged)-Kinase deficient mutant (K55M) of Human mitogen-activated protein kinase 9 (MAPK9), transcript variant JNK2-a2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: JNK2
• Synonyms: JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM9; SAPK; SAPK1a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002752, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGACAGTAAATGTGACAGTCAGTTTTATAGTGTGCAAGTGGCAGACTCAACCTTCACTGTCCTAA
  AACGTTACCAGCAGCTGAAACCAATTGGCTCTGGGGCCCAAGGGATTGTTTGTGCTGCATTTGATACAGT
  TCTTGGGATAAATGTTGCAGTCAAGAAACTAAGCCGTCCTTTTCAGAACCAAACTCATGCAAAGAGAGCT
  TATCGTGAACTTGTCCTCTTAAAATGTGTCAATCATAAAAATATAATTAGTTTGTTAAATGTGTTTACAC
  CACAAAAAACTCTAGAAGAATTTCAAGATGTGTATTTGGTTATGGAATTAATGGATGCTAACTTATGTCA
  GGTTATTCACATGGAGCTGGATCATGAAAGAATGTCCTACCTTCTTTACCAGATGCTTTGTGGTATTAAA
  CATCTGCATTCAGCTGGTATAATTCATAGAGATTTGAAGCCTAGCAACATTGTTGTGAAATCAGACTGCA
  CCCTGAAGATCCTTGACTTTGGCCTGGCCCGGACAGCGTGCACTAACTTCATGATGACCCCTTACGTGGT
  GACACGGTACTACCGGGCGCCCGAAGTCATCCTGGGTATGGGCTACAAAGAGAACGTTGATATCTGGTCA
  GTGGGTTGCATCATGGGAGAGCTGGTGAAAGGTTGTGTGATATTCCAAGGCACTGACCATATTGATCAGT
  GGAATAAAGTTATTGAGCAGCTGGGAACACCATCAGCAGAGTTCATGAAGAAACTTCAGCCAACTGTGAG
  GAATTATGTCGAAAACAGACCAAAGTATCCTGGAATCAAATTTGAAGAACTCTTTCCAGATTGGATATTC
  CCATCAGAATCTGAGCGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCAAAAATGTTAGTGA
  TTGATCCTGACAAGCGGATCTCTGTAGACGAAGCTCTGCGTCACCCATACATCACTGTTTGGTATGACCC
  CGCCGAAGCAGAAGCCCCACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAACATGCAATTGAA
  GAATGGAAAGAGCTAATTTACAAAGAAGTCATGGATTGGGAAGAAAGAAGCAAGAATGGTGTTGTAAAAG
  ATCAGCCTTCAGATGCAGCAGTAAGTAGCAACGCCACTCCTTCTCAGTCTTCATCGATCAATGACATTTC
  ATCCATGTCCACTGAGCAGACGCTGGCCTCAGACACAGACAGCAGTCTTGATGCCTCGACGGGACCCCTT
  GAAGGCTGTCGATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002752 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAACTTGCCCACCC
  TTCGGGATATTGCAGGACGCTGCATCATGAGCGACAGTAAATGTGACAGTCAGTTTTATAGTGTGCAAGT
  GGCAGACTCAACCTTCACTGTCCTAAAACGTTACCAGCAGCTGAAACCAATTGGCTCTGGGGCCCAAGGG
  ATTGTTTGTGCTGCATTTGATACAGTTCTTGGGATAAATGTTGCAGTCATGAAACTAAGCCGTCCTTTTC
  AGAACCAAACTCATGCAAAGAGAGCTTATCGTGAACTTGTCCCTCTTAAAATGTGTCAATCATAAAAATA
  ATAATTAGTTTTGTTAAATGGTGTTTACACAACAAAAAACTCTAAAAGATTTCAAGAGGTGTATTTTGTT
  TATGGAATAAATGATGCTACTTAATGTCAGGTATTAAATGGAGCTGGTCATGAAGAATGGCCTACCTCCT
  TTACCAAATGCTTTGTGATTTAAAATCGGCATTCACGTGTAAATTCATAGAGATTGACCCTACCACATGT
  GTGTGATTCAACTGCCCCTGATATCTGATTTGGCGGCCGGCGCGTGCATACTTGAGAACCTACGTGTACG
  GTACCGCGCGCAATCTCTCGGTGGGCTAAGAGCGTATGGCAGTGTGCACGGAAAGTGAAGTGTGTATTCG
  GCGCGCATTATGGAAATTTGACCGGCACTCGATTTGAAACTCCCTCGAATTGCAACAAGTGGACTTAACA
  TTCTTCGA
  
  Kinase domain sequence

  >SC323575 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAACTTGCCCACCCTTCGGG
  ATATTGCAGGACGCTGCATCATGAGCGACAGTAAATGTGACAGTCAGTTTTATAGTGTGCAAGTGGCAGA
  CTCAACCTTCACTGTCCTAAAACGTTACCAGCAGCTGAAACCAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_002752
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002752.3, NP_002743.3
• RefSeq Size: 1942 bp
• RefSeq ORF: 1275 bp
• Locus ID: 5601
• UniProt ID: P45984
• Cytogenetics: 5q35.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (JNK2-a2) encodes the longer of the two JNK2 alpha isoforms (JNK2 alpha2). The JNK2-a2 variant differs from the JNK2-b2 variant in the use of an alternate internal coding exon of the same length. Thus, JNK2 alpha2 isoform is the same length as JNK2 beta2 isoform, with a few aa differences in an internal protein segment. Variants JNK2-a2 and 8 both encode the same isoform (alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.