ROR2 (NM_004560) Human Untagged Clone

CAT#: SC323522

ROR2 (untagged)-Kinase deficient mutant (K507M) of Human receptor tyrosine kinase-like orphan receptor 2 (ROR2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROR2
• Synonyms: BDB; BDB1; NTRKR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323522 sequence for NM_004560 edited (data generated by NextGen Sequencing)
  ATGGCCCGGGGCTCGGCGCTCCCGCGGCGGCCGCTGCTGTGCATCCCGGCCGTCTGGGCG
  GCCGCCGCGCTTCTGCTCTCAGTGTCCCGGACTTCAGGTGAAGTGGAGGTTCTGGATCCG
  AACGACCCTTTAGGACCCCTTGATGGGCAGGACGGCCCGATTCCAACTCTGAAAGGTTAC
  TTTCTGAATTTTCTGGAGCCAGTAAACAATATCACCATTGTCCAAGGCCAGACGGCAATT
  CTGCACTGCAAGGTGGCAGGAAACCCACCCCCTAACGTGCGGTGGCTAAAGAATGATGCC
  CCGGTGGTGCAGGAGCCGCGGCGGATCATCATCCGGAAGACAGAATATGGTTCACGACTG
  CGAATCCAGGACCTGGACACGACAGACACTGGCTACTACCAGTGCGTGGCCACCAACGGG
  ATGAAGACCATTACCGCCACTGGCGTCCTGTTTGTGCGGCTGGGTCCAACGCACAGCCCA
  AATCATAACTTTCAGGATGATTACCACGAGGATGGGTTCTGCCAGCCTTACCGGGGAATT
  GCCTGTGCACGCTTCATTGGCAACCGGACCATTTATGTGGACTCGCTTCAGATGCAGGGG
  GAGATTGAAAACCGAATCACAGCGGCCTTCACCATGATCGGCACGTCTACGCACCTGTCG
  GACCAGTGCTCACAGTTCGCCATCCCATCCTTCTGCCACTTCGTGTTTCCTCTGTGCGAC
  GCGCGCTCCCGGGCACCCAAGCCGCGTGAGCTGTGCCGCGACGAGTGCGAGGTGCTGGAG
  AGCGACCTGTGCCGCCAGGAGTACACCATCGCCCGCTCCAACCCGCTCATCCTCATGCGG
  CTTCAGCTGCCCAAGTGTGAGGCGCTGCCCATGCCTGAGAGCCCCGACGCTGCCAACTGC
  ATGCGCATTGGCATCCCAGCCGAGAGGCTGGGCCGCTACCATCAGTGCTATAACGGCTCA
  GGCATGGATTACAGAGGAACGGCAAGCACCACCAAGTCAGGCCACCAGTGCCAGCCGTGG
  GCCCTGCAGCACCCCCACAGCCACCACCTGTCCAGCACAGACTTCCCTGAGCTTGGAGGG
  GGGCACGCCTACTGCCGGAACCCCGGAGGCCAGATGGAGGGCCCCTGGTGCTTTACGCAG
  AATAAAAACGTACGCATGGAACTGTGTGACGTACCCTCGTGTAGTCCCCGAGACAGCAGC
  AAGATGGGGATTCTGTACATCTTGGTCCCCAGCATCGCAATTCCACTGGTCATCGCTTGC
  CTTTTCTTCTTGGTTTGCATGTGCCGGAATAAGCAGAAGGCATCTGCGTCCACACCGCAG
  CGGCGACAGCTGATGGCCTCGCCCAGCCAAGACATGGAAATGCCCCTCATTAACCAGCAC
  AAACAGGCCAAACTCAAAGAGATCAGCCTGTCTGCGGTGAGGTTCATGGAGGAGCTGGGA
  GAGGACCGGTTTGGGAAAGTCTACAAAGGTCACCTGTTCGGCCCTGCCCCGGGGGAGCAG
  ACCCAGGCTGTGGCCATCATGACGCTGAAGGACAAAGCGGAGGGGCCCCTGCGGGAGGAG
  TTCCGGCATGAGGCTATGCTGCGAGCACGGCTGCAACACCCCAACGTCGTCTGCCTGCTG
  GGCGTGGTGACCAAGGACCAGCCCCTGAGCATGATCTTCAGCTACTGTTCGCACGGCGAC
  CTCCACGAATTCCTGGTCATGCGCTCGCCGCACTCGGACGTGGGCAGCACCGATGATGAC
  CGCACGGTGAAGTCCGCCCTGGAGCCCCCCGACTTCGTGCACCTTGTGGCACAGATCGCG
  GCGGGGATGGAGTACCTATCCAGCCACCACGTGGTTCACAAGGACCTGGCCACCCGCAAT
  GTGCTAGTGTACGACAAGCTGAACGTGAAGATCTCAGACTTGGGCCTCTTCCGAGAGGTG
  TATGCCGCCGATTACTACAAGCTGCTGGGGAACTCGCTGCTGCCTATCCGCTGGATGGCC
  CCAGAGGCCATCATGTACGGCAAGTTCTCCATCGACTCAGACATCTGGTCCTACGGTGTG
  GTCCTGTGGGAGGTCTTCAGCTACGGCCTGCAGCCCTACTGCGGGTATTCCAACCAGGAT
  GTGGTGGAGATGATCCGGAACCGGCAGGTGCTGCCTTGCCCCGATGACTGTCCCGCCTGG
  GTGTATGCCCTCATGATCGAGTGCTGGAACGAGTTCCCCAGCCGGCGGCCCCGCTTCAAG
  GACATCCACAGCCGGCTCCGAGCCTGGGGCAACCTTTCCAACTACAACAGCTCGGCGCAG
  ACCTCGGGGGCCAGCAACACCACGCAGACCAGCTCCCTGAGCACCAGCCCAGTGAGCAAT
  GTGAGCAACGCCCGCTACGTGGGGCCCAAGCAGAAGGCCCCGCCCTTCCCACAGCCCCAG
  TTCATCCCCATGAAGGGCCAGATCAGACCCATGGTGCCCCCGCCGCAGCTCTACATCCCC
  GTCAACGGCTACCAGCCGGTGCCGGCCTATGGGGCCTACCTGCCCAACTTCTACCCGGTG
  CAGATCCCAATGCAGATGGCCCCGCAGCAGGTGCCTCCTCAGATGGTCCCCAAGCCCAGC
  TCACACCACAGTGGCAGTGGCTCCACCAGCACAGGCTACGTCACCACGGCCCCCTCCAAC
  ACATCCATGGCAGACAGGGCAGCCCTGCTCTCAGAGGGCGCTGATGACACACAGAACGCC
  CCAGAAGATGGGGCCCAGAGCACCGTGCAGGAAGCAGAGGAGGAGGAGGAAGGCTCTGTC
  CCAGAGACTGAGCTGCTGGGGGACTGTGACACTCTGCAGGTGGACGAGGCCCAAGTCCAG
  CTGGAAGCTTGA
  
  Clone variation with respect to NM_004560.3
  733 a=>g;1520 a=>t;1521 a=>g;2088 c=>t;2455 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004560 unedited
  ACGTCCGACCTACCAACGAGCGGAGGCGCGCACGGCGGGAGGACAACACAAGCAGAGCACGCCCAGCGAA
  CCGTCAGAGAAACGAAACACGACACACAAAAGGGCGGCCGCGAACACGGCACCAGGGACCTCGAAGCGGA
  CCCGTCCGCGAAGCGCCAGGGAGAAGGAGGAGCGGACGCATCGTAGAAAGGGGTTGGTGGCGCCCGACCC
  CGCGCCCCGGCCCGAAGCTCTGAGGGCTTCCCGGCCCCCACTGCCTGCGGCATGGCCCGGGGCTCGGCGC
  TCCCCGCGGCGGCCGCTGCTGTGCATCCCGGGCCGTCTGGGCGGGCCGCCCGCGCTTCTGCTCTCAGTGT
  CCGGGACTTTCAGGTGAAATTGGAGGTTCTGGGATCCGAACGACCCTTTAGAACCCCTTGAGGGCCAGAA
  CGGCCCAATTCCACCTCTGAAAGGTTACTTTCTAATTTTTCTGAACCCAGTAAACAATATCCCCATTGTC
  CAGGGCCGACGGCAATTCCGCCCTGCAAGGTGGGCGGGAACCCCCCCCCTTACGTGCCGGGGCCTAAGAA
  TTAAGCCCCCGGGGGGCAAGAGCCCCGGCCGGATCAACTTCCCGAAAAACGAAATGGGTTACGACTGCGG
  AATCCAGGACCTGGGCACCACAGACACTGGGTTCTATCCATGCGTGGGCCCCCCCCGGGTGAAAACACTT
  TCCCCCCTGGGGTCCTGTGTTTGCGCGCGGGGTCCCACCCACCCCCATATTAACTTTAGAGAATATCACG
  AGATGGGGGTCTGCGCCCCCTATCGCGGGATGCCGTGGCGCCTCTCTGCACCGGCACTTATGGACCTTCA
  GCCGGGGGATTAGAACCATCACGGCCTTCACGTATGGCGCTCCACTGTCGACATGTCCAGTTGCATCACT
  CTTGCAATAGGTACCTGGCACGCGCTTCTGGACACAGCCGTAGATA
  
  Kinase domain sequence

  >SC323522 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGCAGMGGTTGGGAGTCTACARGGTCACCTGTTCGGCCCTGCCCCGGGGGAGCAGACCCAGGCTGTGGCC
  ATCATGACGCTGAAGGACAAAGCGGAGGGGCCCCTGCGGGAGGAGTTCCGGCATGAGGCTATGCTGCGAG
  CACGGCTGCAACACCCCAACGTCGTCTGCCTGCTGGGCGTGGTGACCAAGGACCAGCCCCTGAGCATGAT
  CTTCAGCTACTGTTCGCACGGCGACCTCCACGAATTCCTGGTCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_004560
• Insert Size: 3600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004560.2, NP_004551.2
• RefSeq Size: 4099 bp
• RefSeq ORF: 2832 bp
• Locus ID: 4920
• UniProt ID: Q01974
• Cytogenetics: 9q22.31
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).