S6K1 (RPS6KB1) (NM_003161) Human Untagged Clone

CAT#: SC323401

RPS6KB1 (untagged)-Kinase deficient mutant (K123M) of Human ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S6K1
• Synonyms: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003161, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGCGACGAAGGAGGCGGGACGGCTTTTACCCAGCCCCGGACTTCCGAGACAGGGAAGCTGAGGACA
  TGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAGGACGCGGGCTCTGAGGATGAGCTGGAGGAGGG
  GGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCATGGAACATTGTGAG
  AAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTAC
  TTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATACTGG
  GAAAATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTAAGAAATGCTAAAGATACAGCTCATACA
  AAAGCAGAACGGAATATTCTGGAGGAAGTAAAGCATCCCTTCATCGTGGATTTAATTTATGCCTTTCAGA
  CTGGTGGAAAACTCTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTATTTATGCAGTTAGAAAGAGA
  GGGAATATTTATGGAAGACACTGCCTGCTTTTACTTGGCAGAAATCTCCATGGCTTTGGGGCATTTACAT
  CAAAAGGGGATCATCTACAGAGACCTGAAGCCGGAGAATATCATGCTTAATCACCAAGGTCATGTGAAAC
  TAACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACAGTCACACACACATTTTGTGGAACAAT
  AGAATACATGGCCCCTGAAATCTTGATGAGAAGTGGCCACAATCGTGCTGTGGATTGGTGGAGTTTGGGA
  GCATTAATGTATGACATGCTGACTGGAGCACCCCCATTCACTGGGGAGAATAGAAAGAAAACAATTGACA
  AAATCCTCAAATGTAAACTCAATTTGCCTCCCTACCTCACACAAGAAGCCAGAGATCTGCTTAAAAAGCT
  GCTGAAAAGAAATGCTGCTTCTCGTCTGGGAGCTGGTCCTGGGGACGCTGGAGAAGTTCAAGCTCATCCA
  TTCTTTAGACACATTAACTGGGAAGAACTTCTGGCTCGAAAGGTGGAGCCCCCCTTTAAACCTCTGTTGC
  AATCTGAAGAGGATGTAAGTCAGTTTGATTCCAAGTTTACACGTCAGACACCTGTCGACAGCCCAGATGA
  CTCAACTCTCAGTGAAAGTGCCAATCAGGTCTTTCTGGGTTTTACATATGTGGCTCCATCTGTACTTGAA
  AGTGTGAAAGAAAAGTTTTCCTTTGAACCAAAAATCCGATCACCTCGAAGATTTATTGGCAGCCCACGAA
  CACCTGTCAGCCCAGTCAAATTTTCTCCTGGGGATTTCTGGGGAAGAGGTGCTTCGGCCAGCACAGCAAA
  TCCTCAGACACCTGTGGAATACCCAATGGAAACAAGTGGCATAGAGCAGATGGATGTGACAATGAGTGGG
  GAAGCATCGGCACCACTTCCAATACGACAGCCGAACTCTGGGCCATACAAAAAACAAGCTTTTCCCATGA
  TCTCCAAACGGCCAGAGCACCTGCGTATGAATCTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003161 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATATCGTCGACCCACGCG
  TCCGCGGACGCGTGGGTGGCGGCAGCGGCTGTGGTGGCTGCGGCGGGTCCGGGCCCATGAGGCGACGAAG
  GAGGCGGGACGGCTTTTACCCAGCCCCGGACTTCCGAGACAGGGAAAGCTGAGGACATGGCAGGGAGTGT
  TTGACATAGACCTGGACCAGCCCAGAGGACGCGGGGCTCTGAGGATGAACCTGAAGGAAGGGGGGTCAAT
  TTAAATTGAAACCTTGGAACCAGGGGGGAATTGGGGACCATATGACCCTTGGCTGGGAACATTGGGGGGA
  AATTGAGAATCCTAAAAAACTATTGTGGAACGAAAGGGCAAAAAAAAAATCAAACCAAAAGGTTTTTAAG
  CTATTTTCGGGTACTGGGTAAAGGGGGGCTTGGGAAAGGGTTTTTCCAGTTCCAAAAGTTCCCGGGAGCC
  ATTCCGTGGGAAAAATTTTTGCCCGGGGGGGGGCTTAAAAAGGGACGGGGATTTAAAAATGGCAAAAAAA
  CCCGCCTACCCAACCCCAAAGGGAATTTTTGGGGGGAAAAAAAAAACCCCCCCTTCCCGGGGGTTAAATT
  TTGCCCTTTTCACAGGGGGGGGAAAACCTCCCCCTCCCCCGGGATTCCCTGGGGGAGGAAAATTTTTTTC
  TTTTTAAGAGAAGGGAGGATTTTTTTGGGAAGACAGCCCGGCGTTTTCTTTGTGGAAAAACTCTGCGTGT
  GTTGTGGGGCTTCTTCTCTAAGAGAGCATCTCTCGAGACTCTGCGCCGCAGGATATGTGCTGTATCACGC
  GAGTGTGTGATGATCAGCTTGCTGTGACGACGAATATCTGTATGTGACGACCGCCCACCTCTTGTGAGAG
  AGCAATGATACGACGTCACATA
  
  Kinase domain sequence

  >SC323401 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AWKSSWRRWMWTCGTGTKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGT
  TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATATCGTCGA
  CCCACGCGTCCGCGGACGCGTGGGTGGCGGCAGCGGCTGTGGTGGCTGCGGCGGGTCCGGGCCCATGAGG
  CGACGAAGGAGGCGGGACGGCTTTTACCCAGCCCCGGACTTCCGA
  
• Restriction Sites: Please inquire
• ACCN: NM_003161
• Insert Size: 5100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003161.2, NP_003152.1
• RefSeq Size: 5332 bp
• RefSeq ORF: 1578 bp
• Locus ID: 6198
• UniProt ID: P23443
• Cytogenetics: 17q23.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway
• Gene Summary: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) can initiate translation from two alternate in-frame AUG start codons. The isoform represented in this RefSeq (a, also known as p85 or alpha I) is derived from the first AUG start codon, and is the longest isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.