PIM1 (NM_002648) Human Untagged Clone

CAT#: SC323357

PIM1 (untagged)-Kinase deficient mutant (K67M) of Human pim-1 oncogene (PIM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIM1
• Synonyms: PIM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323357 sequence for NM_002648 edited (data generated by NextGen Sequencing)
  ATGCTCTTGTCCAAAATCAACTCGCTTGCCCACCTGCGCGCCGCGCCCTGCAACGACCTG
  CACGCCACCAAGCTGGCGCCCGGCAAGGAGAAGGAGCCCCTGGAGTCGCAGTACCAGGTG
  GGCCCGCTACTGGGCAGCGGCGGCTTCGGCTCGGTCTACTCAGGCATCCGCGTCTCCGAC
  AACTTGCCGGTGGCCATCATGCACGTGGAGAAGGACCGGATTTCCGACTGGGGAGAGCTG
  CCTAATGGCACTCGAGTGCCCATGGAAGTGGTCCTGCTGAAGAAGGTGAGCTCGGGTTTC
  TCCGGCGTCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAGTTTCGTCCTGATCCTG
  GAGAGGCCCGAGCCGGTGCAAGATCTCTTCGACTTCATCACGGAAAGGGGAGCCCTGCAA
  GAGGAGCTGGCCCGCAGCTTCTTCTGGCAGGTGCTGGAGGCCGTGCGGCACTGCCACAAC
  TGCGGGGTGCTCCACCGCGACATCAAGGACGAAAACATCCTTATCGACCTCAATCGCGGC
  GAGCTCAAGCTCATCGACTTCGGGTCGGGGGCGCTGCTCAAGGACACCGTCTACACGGAC
  TTCGATGGGACCCGAGTGTATAGCCCTCCAGAGTGGATCCGCTACCATCGCTACCATGGC
  AGGTCGGCGGCAGTCTGGTCCCTGGGGATCCTGCTGTATGATATGGTGTGTGGAGATATT
  CCTTTCGAGCATGACGAAGAGATCATCAGGGGCCAGGTTTTCTTCAGGCAGAGGGTCTCT
  TCAGAATGTCAGCATCTCATTAGATGGTGCTTGGCCCTGAGACCATCAGATAGGCCAACC
  TTCGAAGAAATCCAGAACCATCCATGGATGCAAGATGTTCTCCTGCCCCAGGAAACTGCT
  GAGATCCACCTCCACAGCCTGTCGCCGGGGCCCAGCAAATAG
  
  Clone variation with respect to NM_002648.3
  200 a=>t;201 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002648 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGCGGCGGGACCGGC
  AGCAGCAGCAGCAGCAGCAGCAGCAACCACTAGCCTCCTGCCCCGCGGCGCTGCCGCACGAGCCCCACGA
  GCCGCTCACCCCGCCGTTCTCAGCGCTGCCCGACCCCGCTGGCGCGCCCTCCCGCCGCCCAGTCCCGGCA
  GCGCCCTCAGTTGTCCTCCGACTCGCCCTCGGCCTTCCGCGCCAGCCGCAGCGACAGCCGCAACTGCCTC
  CCGGCAGCCACAGCCACAGCCCCAGCCCCAGGCATAGCCTTGGGCACAGCCCCGGTCCCGGCTCTGGGGG
  GAAGCTCCTTGGGGGAACGGTCCTGGCCCGAACATCTGGGAAGGTTGGGATGGCTTTTGTCAAAAATCAC
  CTCGCTTGCCCACTGGGCCCGCCGCGCCTTGAAGAACCTGCACGCCCCCAGCTGGGGCCCCGGGAAGGGA
  AAGGAACCCCGGGATTCGCTTTCCCGTGGGGCCCCGTTTGGGGGCAGGGGGGGTTTGCTCTGGTTATACT
  GGGGCTTCCGTTTTTGAAACATTTGGGGGGGGCCCATTTGCCCTTGAAAAACACCGTTTTTCCAGGGGGG
  AAACCGCCATGGACCCCACTGCCCCAAGAGAGTGCCCCTGATAAAAAAGGACACTGTGTTTCCACGGCTT
  ATGCGCTCGAGAGTGGTAAAGAGCCAATTTTCTCTCTGTCTAGAGAGCGCCGCGCTATTTCTCTTATCAC
  GAGAGGACACCGAGAGTCGGAGCGCTCCTTGAGAGTGGAGCAGCGCAGTGGACACGAGGG
  
  Kinase domain sequence

  >SC323357 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKACKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGGCGGCGGGACCGGCAGCA
  GCAGCAGCAGCAGCAGCAGCAACCACTAGCCTCCTGCCCCGCGGCGCTGCCGCACGAGCCCCACGAGCCG
  CTCACCCCGCCGTTCTCAGCGCTGCCCGACCCCGCTGGCGCGCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_002648
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002648.2, NP_002639.1
• RefSeq Size: 2684 bp
• RefSeq ORF: 942 bp
• Locus ID: 5292
• UniProt ID: P11309
• Cytogenetics: 6p21.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternate in-frame, translation initiation codons. This RefSeq represents the shorter isoform (2, also known as Pim-1S) derived from the use of a downstream AUG (at nt 431-433). Pim-1S has been shown to localize predominantly in the nucleus (PMID:16186805).