IKK alpha (CHUK) (NM_001278) Human Untagged Clone

CAT#: SC323326

CHUK (untagged)-Kinase deficient mutant (K44M) of Human conserved helix-loop-helix ubiquitous kinase (CHUK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IKK alpha
• Synonyms: BPS2; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323326 sequence for NM_001278 edited (data generated by NextGen Sequencing)
  ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCCCTGGGAGATGCGGGAGCGG
  CTGGGCACCGGCGGCTTCGGGAACGTCTGTCTGTACCAGCATCGGGTACTTGATCTCAAA
  ATAGCAATTATGTCTTGTCGCCTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCAT
  GAAATCCAGATTATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCT
  GAAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATACTGTTCTGGA
  GGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTGTGGACTTAAAGAAAGCCAG
  ATACTTTCTTTACTAAGTGATATAGGGTCTGGGATTCGATATTTGCATGAAAACAAAATT
  ATACATCGAGATCTAAAACCTGAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATA
  CATAAAATAATTGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCT
  TTTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCTTACACAGCC
  ACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATGTATTGCTGGATATAGGCCT
  TTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGATTAAGAAGAAGGATCCAAAG
  TGTATATTTGCATGTGAAGAGATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAA
  CCAAATAGCCTTTGTAGTTTAGTAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTG
  AATTGGGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAGCCAAGATGT
  TTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACACATCCTAAATATGACTTCT
  GCAAAGATAATTTCTTTTCTGTTACCACCTGATGAAAGTCTTCATTCACTACAGTCTCGT
  ATTGAGCGTGAAACTGGAATAAATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATT
  TCTCTGGATCCTCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGAT
  AGCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCATTTGCTTCC
  AGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAGCAAAATACAGCTTCCAATT
  ATACAGCTGCGTAAAGTGTGGGCTGAAGCAGTGCACTATGTGTCTGGACTAAAAGAAGAC
  TATAGCAGGCTCTTTCAGGGACAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCT
  AACTTAACAAAAATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTG
  GAGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAGATGACGTAT
  GGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAATGGAAGAAAAGGCCATCCAC
  TATGCTGAGGTTGGTGTCATTGGATACCTGGAGGATCAGATTATGTCTTTGCATGCTGAA
  ATCATGGAGCTACAGAAGAGCCCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTG
  GAACAGCGTGCCATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTAC
  AGTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAGGACCGTGTG
  CTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTGTAAGCAGAAGATTATTGAT
  CTACTCCCTAAGGTGGAAGTGGCCCTCAGTAATATCAAAGAAGCTGACAATACTGTCATG
  TTCATGCAGGGAAAAAGGCAGAAAGAAATATGGCATCTCCTTAAAATTGCCTGTACACAG
  AGTTCTGCCCGGTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCA
  GCATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTGGTAACTCCT
  CAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTTGAACTGCCTTGGCCATTTA
  AGCACTATTATTCATGAGGCAAATGAGGAACAGGGCAATAGTATGATGAATCTTGATTGG
  AGTTGGTTAACAGAATGA
  
  Clone variation with respect to NM_001278.3
  107 a=>t;131 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001278 unedited
  TCGGTACGACGAACAACGGGCGGAGGCGGCACGGGAGGGCCACACAAGCAGACACGAAAGCGAACCGACA
  GAGTACGAAAACGACACACAAAAGGGCGGCCGCGAACACGGCACGAGGCGGCCACGGAACAACCGCGGAA
  CCCGAGGCCGCCCGCCCACCCGCCCCACGGAGCGGCCCCCGGGGCAGCGGCCGGGCGCGGGCGGGCCCTG
  GGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTTCGGGAACGTTCTGTCTGTACCAGCATCGGGTACTTG
  ATTCTCAAAATAGCAATTATGTCTTTGTCGCCTAGAGCTAAGTACCAAAAAACAGAGAAACGAATGGTTG
  CCATGAAATCCAGATTATGAAAGAAAGTTGACCCTGGCCATGGTTGGTAAAGGCCGGGGAGGTTCCTGAG
  AAATTGATTATTTTTGATTCTGGATGTGCCTCTTCTACCATGGGATTACGGTTCGGAAGGAAATCTCCAA
  AGGCGGCTCACCAAACCAAAAAATGTTTGGGGACTTAGAAAACCCAGTACCTTTTTTTCTTAATTGATTA
  GGGCCGGGTTCGATTTTTCATGAAAAAAAATTTTAATTCGATCTAAAACCTGAAACCAAGTCTTCCGAAT
  TTGTGGGAAGATATACATATAATATATGATCTGGAATGCCCAGATATGGTCAAGAGATCTGGGAAACTTT
  TGGAAACTGAGATCTGCCCAAGCTCTTGGATACCTACAGCACTGTGATATTGACTTGGACATGTATGATG
  ATGCGGATAAGCCTTTTGCTCTCGGACATACTGCTGGAGATAGAGAAGTCCAATGTATGCTGAAGAGTCG
  GAATCGTGTAAACAATACACAACCTGAATAAG
  
  Kinase domain sequence

  >SC323326 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCTTGGAACAACTGTG
  GAACCTGAGGCCGCTTGCCCTCCCGCCCCATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC
  CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGT
  
• Restriction Sites: Please inquire
• ACCN: NM_001278
• Insert Size: 3670 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278.3, NP_001269.3
• RefSeq Size: 3539 bp
• RefSeq ORF: 2238 bp
• Locus ID: 1147
• UniProt ID: O15111
• Cytogenetics: 10q24.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]