CTNNA3 (NM_001127384) Human Untagged Clone

CAT#: SC323229

CTNNA3 (untagged)-Human catenin (cadherin-associated protein), alpha 3 (CTNNA3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTNNA3
• Synonyms: ARVD13; VR22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323229 representing NM_001127384.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCAGCTGAAACACCAATCACATTGAATATCGATCCTCAGGATCTGCAGGTCCAAACATTCACCGTG
  GAGAAGCTACTGGAGCCTCTCATAATCCAGGTTACCACACTTGTAAACTGTCCCCAGAACCCTTCCAGC
  AGGAAAAAAGGACGTTCGAAAAGAGCCAGTGTCCTTCTAGCTTCTGTGGAGGAAGCAACTTGGAATTTA
  TTAGACAAGGGAGAGAAGATTGCCCAGGAAGCTACAGTTTTAAAGGATGAGCTTACGGCTTCACTTGAG
  GAAGTTCGCAAAGAAAGTGAAGCTCTGAAAGTATCAGCTGAGAGATTTACAGATGACCCCTGTTTTCTC
  CCAAAAAGGGAGGCTGTGGTTCAAGCTGCCCGTGCCTTGCTGGCTGCGGTGACGAGACTCCTTATCCTT
  GCGGACATGATTGATGTCATGTGCCTCTTGCAACATGTGTCAGCTTTTCAAAGGACATTTGAGTCTCTC
  AAAAATGTTGCCAACAAATCTGACCTCCAGAAAACCTACCAGAAGCTTGGGAAGGAGCTGGAAAATTTG
  GATTATTTAGCCTTCAAACGTCAGCAGGACTTAAAATCTCCAAATCAGAGAGATGAAATTGCAGGAGCC
  CGAGCTTCACTGAAGGAGAACTCTCCCCTCTTGCATTCAATTTGTTCAGCTTGTTTGGAGCATTCTGAT
  GTTGCTTCCCTCAAAGCAAGCAAGGACACAGTTTGTGAAGAAATTCAGAATGCTCTCAATGTAATTTCA
  AATGCTTCACAAGGGATCCAGAATATGACAACCCCACCAGAACCTCAGGCAGCAACCCTGGGAAGTGCC
  CTTGATGAGCTGGAGAATTTAATTGTCCTGAATCCACTCACAGTAACTGAGGAGGAAATACGACCATCA
  CTAGAGAAACGCCTTGAAGCCATTATCAGTGGGGCTGCTCTGCTGGCGGATTCTTCATGTACGAGGGAC
  TTACACCGAGAGCGGATTATCGCAGAATGCAACGCCATTCGCCAGGCTCTTCAGGATCTGCTTTCAGAG
  TACATGAACAACGCTGGAAAAAAAGAAAGGAGTAATACCCTGAATATTGCTTTAGACAACATGTGTAAG
  AAGACAAGAGACCTTCGCAGACAGCTCCGCAAGGCTATTATAGATCATGTGTCAGACTCTTTCCTGGAT
  ACGACAGTCCCTCTTTTGGTTCTCATTGAAGCTGCTAAGAATGGCCGGGAAAAGGAAATAAAAGAATAT
  GCTGCGATATTTCATGAACACACCAGCAGGCTTGTAGAGGTGGCAAATCTTGCTTGTTCCATGTCAACA
  AATGAAGATGGAATTAAAATTGTCAAAATTGCAGCCAATCATTTGGAAACCTTGTGTCCACAGATTATT
  AATGCTGCACTTGCTTTGGCTGCAAGACCCAAAAGTCAAGCGGTCAAAAACACCATGGAAATGTACAAG
  CGTACATGGGAGAATCATATACATGTCCTCACTGAAGCCGTAGATGACATTACAAGCATTGATGACTTC
  CTTGCTGTATCTGAAAGCCATATCTTGGAAGATGTCAACAAGTGTATCATAGCCTTAAGAGACCAGGAT
  GCTGATAATTTAGACCGTGCTGCGGGTGCTATCAGAGGCCGGGCAGCAAGAGTTGCTCACATCGTCACG
  GGTGAAATGGACAGTTACGAGCCAGGGGCTTACACGGAAGGTGTAATGAGAAATGTTAACTTCCTTACA
  AGTACTGTAATTCCTGAATTTGTAACACAAGTGAATGTTGCCTTGGAAGCCTTAAGCAAAAGCTCATTG
  AATGTGTTGGATGATAATCAATTTGTGGACATCTCAAAGAAGATCTATGATACAATTCATGATATCAGA
  TGTTCAGTCATGATGATTCGGACCCCAGAGGAACTGGAGGATGTTTCTGACCTTGAAGAGGAACACGAG
  GTCCGCAGTCACACCAGCATTCAGACCGAAGGGAAAACTGATAGGGCTAAGATGACTCAACTGCCTGAG
  GCAGAAAAAGAAAAGATTGCTGAGCAAGTTGCTGATTTCAAGAAAGTAAAGAGTAAGCTGGATGCTGAG
  ATTGAGATATGGGATGATACAAGCAACGACATCATTGTTCTGGCCAAGAACATGTGTATGATCATGATG
  GAGATGACAGACTTCACTAGGGGCAAAGGACCACTAAAGCATACAACTGATGTGATCTATGCAGCGAAA
  ATGATATCAGAATCAGGATCAAGGATGGATGTCCTTGCTCGGCAGATTGCTAATCAGTGCCCAGATCCA
  TCTTGTAAACAGGACTTGTTGGCCTACCTGGAACAGATTAAGTTCTACTCCCACCAACTGAAAATCTGC
  AGTCAAGTTAAAGCTGAGATCCAGAACCTGGGAGGAGAGCTCATCATGTCAGCTTTGGACAGTGTCACA
  TCCCTGATCCAAGCAGCCAAAAATTTAATGAATGCTGTAGTGCAAACAGTGAAAATGTCTTACATTGCC
  TCAACCAAGATCATCCGAATCCAGAGTCCTGCTGGGCCCCGGCACCCAGTTGTGATGTGGAGAATGAAG
  GCTCCTGCAAAAAAACCCTTGATTAAAAGAGAGAAGCCAGAGGAAACGTGTGCAGCTGTCAGACGAGGC
  TCAGCAAAGAAAAAAATCCATCCATTGCAAGTCATGAGTGAATTTAGAGGAAGACAAATCTACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127384
• Insert Size: 2688 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127384.2
• RefSeq Size: 10663 bp
• RefSeq ORF: 2688 bp
• Locus ID: 29119
• UniProt ID: Q9UI47
• Cytogenetics: 10q21.3
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 99.8 kDa
• Gene Summary: This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.