B3GNT1 (B3GNT2) (NM_006577) Human Untagged Clone

CAT#: SC322639

B3GNT2 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B3GNT1
• Synonyms: 3-Gn-T1; 3-Gn-T2; B3GN-T2; B3GNT; B3GNT-2; B3GNT1; beta-1; beta3Gn-T1; beta3Gn-T2; BETA3GNT; BGnT-2; BGNT2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322639 GGCGCAGCGGCAGCGGCAGCAGCGGCAACAAGTGCCGGAGGCTAGCAGAGCCAAGCCGGA
  GCAGTCCCTGCCGCCGACACCGCCGGGCCGCCCGTCCGGGGCGCCGCGCATGGAGCGTGA
  GCTGCGGCGGTCGCCGGGCTGAGCCGCGCGGAGCGGCCGGGACGTGGATGTGGCCGCGAT
  CTCCCGCCCTTGCCCCCGCCCCGCCGAGCTGGAGCTGCTCCCGGACAAGATATGAGAAAT
  GAGTGTTGGACGTCGAAGAATAAAGTTGTTGGGTATCCTGATGATGGCAAATGTCTTCAT
  TTATTTTATTATGGAAGTCTCCAAAAGCAGTAGCCAAGAAAAAAATGGAAAAGGGGAAGT
  AATAATACCCAAAGAGAAGTTCTGGAAGATATCTACCCCTCCCGAGGCATACTGGAACCG
  AGAGCAAGAGAAGCTGAACCGGCAGTACAACCCCATCCTGAGCATGCTGACCAACCAGAC
  GGGGGAGGCGGGCAGGCTCTCCAATATAAGCCATCTGAACTACTGCGAACCTGACCTGAG
  GGTCACGTCGGTGGTTACGGGTTTTAACAACTTGCCGGACAGATTTAAAGACTTTCTGCT
  GTATTTGAGATGCCGCAATTATTCACTGCTTATAGATCAGCCGGATAAGTGTGCAAAGAA
  ACCTTTCTTGTTGCTGGCGATTAAGTCCCTCACTCCACATTTTGCCAGAAGGCAAGCAAT
  CCGGGAATCCTGGGGCCAAGAAAGCAACGCAGGGAACCAAACGGTGGTGCGAGTCTTCCT
  GCTGGGCCAGACACCCCCAGAGGACAACCACCCCGACCTTTCAGATATGCTGAAATTTGA
  GAGTGAGAAGCACCAAGACATTCTTATGTGGAACTACAGAGACACTTTCTTCAACTTGTC
  TCTGAAGGAAGTGCTGTTTCTCAGGTGGGTAAGTACTTCCTGCCCAGACACTGAGTTTGT
  TTTCAAGGGCGATGACGATGTTTTTGTGAACACCCATCACATCCTGAATTACTTGAATAG
  TTTATCCAAGACCAAAGCCAAAGATCTCTTCATAGGTGATGTGATCCACAATGCTGGACC
  TCATCGGGATAAGAAGCTGAAGTACTACATCCCAGAAGTTGTTTACTCTGGCCTCTACCC
  ACCCTATGCAGGGGGAGGGGGGTTCCTCTACTCCGGCCACCTGGCCCTGAGGCTGTACCA
  TATCACTGACCAGGTCCATCTCTACCCCATTGATGACGTTTATACTGGAATGTGCCTTCA
  GAAACTCGGCCTCGTTCCAGAGAAACACAAAGGCTTCAGGACATTTGATATCGAGGAGAA
  AAACAAAAATAACATCTGCTCCTATGTAGATCTGATGTTAGTACATAGTAGAAAACCTCA
  AGAGATGATTGATATTTGGTCTCAGTTGCAGAGTGCTCATTTAAAATGCTAAAATAGATA
  CAAACTCAATTTTGCATAGAAAGGTGTATTTTGAATAGTTCCCATGTTGTGTTCTCACAC
  TAGAGTAATTTCTATATTAAACCATGAAAATTGCCTTTATGAGTGATACCCATTTGAGGG
  CCTCTAAACCCTTCAATTTGGTACTCACGTGAAGAGGGAAAGCGGAAGATGGTAATTTTT
  TTTATGGATGATATGGCAGGATGATTGGTTCTGATCTTACCGGCTAGTGGTCATTTTTAA
  AAAACTTGTACCCTCTTATCTGAAATCCTGTTTCTGGAATTTGGCCATTTTAAGTGATTT
  TGTTTGCCCTCTTCTATAATATTCCTACTTCCCATAATAATGACTGATTTATTTGTAATT
  CAGGTATTTATAAACCTATTGGCTACAAAGACTTTGTTAAACATTATCCAGTGGTTTTCG
  TGAAATGGAATTATGTTTATTTTTATGGGATTTGGGTAAATTTTAAATTGTCTAGAAAAC
  TGAAATTTCAGTTGTCAGTTGTGGAATTCAGTTTTTCAATTGTGGAAATTTCCTGCCACC
  CCAACAGTATTTTTGTGTGTTAATTAATTTTGCAAAATGAGAATCATGGTGTGACACTCA
  TCTAATTTATCTTGTTGTGATGTTATGGTCATAATAAGGAGAAAGAGGGTTTAATTTTTC
  TTGTATTTGGTTTCCTGGTGGTATCATAGTGTAATTTTAGTATTTGAAAATCAGTGTGAT
  TCCTTAATGGCCAACTGAAGATTGAATTGCCGCTAACAACCATATCGTGTTAGTGAATTT
  TCAATATGGACCAGGAAGGCATATGTATTTTGAACTTGAGTGAAAAGGTTGAAGTTACAG
  ACTTTTGCATAGATGGTTTGTCAATTTAAAATTCCAGAATTTATTATTGCCATATTTTCA
  CATGCTGCTTATACAAGATTATTATTGAGTAGTAACTGCTTCCCTGTCTATGTAGAAGTG
  CCTGTGTTTTTATTTATTGTTCAGATCAAAGACCAAAACATTTTCTTAAATATATTTTAT
  GTAATATTTTATTTGTATACAGTGTTGTTGATGAAATATTTAACTAGAGCATGATATTTT
  AAATGTTAAGGTGTAACATATGTTAAATAAAACTGTTATTTTTGAATTTTAAAATTTGTT
  TTTTGGGGGTATGAACTACTAGAGTTTAAAATTCTGCCAAACTATTACTTATATGTACTA
  TTGTGTAACATACTTTCTTGAAATATTTTTGTTTATAGAATTGAAGGTTCTTATCAGATG
  GGATACTGGGGACTATAAACAATGGAAATAAAGCCACTGTATTTTTAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAAAAAAAAAAAG
• Restriction Sites: Please inquire
• ACCN: NM_006577
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006577.5, NP_006568.2
• RefSeq Size: 2788 bp
• RefSeq ORF: 1194 bp
• Locus ID: 10678
• UniProt ID: Q9NY97
• Cytogenetics: 2p15
• Domains: Galactosyl_T
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 3 both encode the same protein.