Josephin 1 (JOSD1) (NM_014876) Human Untagged Clone

CAT#: SC322360

JOSD1 (untagged)-Human Josephin domain containing 1 (JOSD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Josephin 1
• Synonyms: dJ508I15.2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322360 CCCACGCGTCCGCCCACGCGTCCGCTTACATTTTATGTGTTTTTTTAAATTCTGCACTTT
  GACAGTGGTAGCAAAATAAAAGTAATCCAAAAGTTATTTTTCTATTGAGAAGAGAGAAGA
  GGTGGGTTTTATTTTTTTCCTTTTGATTCCAATCTTTTCTCCCAGTGACAAAGCATAAAT
  CATGGACACCAGAGAATAAGGTGGACCTCAGGAATCCGGAAAAGACTGAGAAGCTTACAT
  TCTTCCTCTAGAGGGAAGAGTGGGGTGTTTATAGCCATCTCTGGAACCTAAAACAAAAAA
  CATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATCATTGGAGCTGCCCCA
  GGCAGCACCCCCACAAATCTACCATGAGAAACAGCGCAGGGAGCTTTGTGCCCTCCACGC
  CCTCAATAACGTCTTCCAGGACAGCAATGCCTTCACCCGGGATACGCTGCAAGAGATTTT
  CCAGAGGTTGTCTCCAAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGG
  CAACTACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCTGTTTG
  GTGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGTCATGGGCTTCATCAT
  GAATCTGCCCTCCAGCCTATGCTGGGGTCCACTGAAACTGCCCCTCAAAAGGCAGCACTG
  GATCTGTGTTCGAGAGGTGGGAGGGGCCTACTACAACCTCGACTCCAAACTCAAGATGCC
  CGAGTGGATTGGAGGCGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAA
  GAACTGTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGGAGGAC
  CGATGTGTAACAGTTCTGCCCAACCTCCCTCTCGCCTCAGCCCCTTCAGTCCTCTGTGAC
  GTGCTGTGGCCTCTACAGTGGGTCTGCCCTTGCCACTTCCCCAAACATCTCATCAAGTTT
  TTCCCCTTCAGATCTGACAGTGCAATAGGACAGACGTGTGGACTGTTATAAGAACTACTC
  AGTGTTTTGTTCCTGGGCAAGGAAGGTAGGAGTTCTGTGCACTTAAGGCCAGTGGTCACA
  AACCCTTGTTTTATTTAAGAGACAGAGGAGAAAGTGGAGCGGGGAGGGAATCCTAGCTTA
  TTTTCCCTTTTCTATGAGGACTTGACACAGGTTCTGCTGAGTTGTCACTGCTGCTCCAGA
  CTCACCTAGAGATGCTGCCTCCACTTTCCATCCTGTCTGGGTCTGAAAACAGTGGGTCTG
  CAGATAGTGCCCACAAACCCCATGTGACTGGTTTGAAGGACCCAGAGCATAAAGGTCTCT
  CAGGAAACCATGTCCAAAACCCTAGCAGCGGTACAGCATGCTGTCTCCAACCCTTATCCC
  CAGGTTTAAGGGTGGTTTATGGCCATACGTGGAGGTTTTTTGTTGTTGTTTTTGAGACTG
  AGTTTCACTCTTGTTGCCCAGGCTGGAGTGCAATGGCACCATCTCGGCTCACTGCAACCT
  CCACCTCCTGGTTCAAGCGATTCTCAGGCCTCAGCTTCCCAAGTAGTTGGGATTACAGGC
  GCCTGCCACCACACCTGGCTAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTG
  GTCAGGCTGGTCTCAAACTCCCGACCTCAAGTGATCTGCCCGCCTTGGCCTCCCAAAGTG
  CTGGGATTACAGGCGTGAGCCACCGCACCCGGCAGAGTTTTATAATGAAAAATTAACTAA
  TATTCTAGTATGAAGTGAGGAGGATACTGAACAGGATGTGGCTAAAGCCAACCTGGGACA
  GCCATGGGGTGGCTTGGTTTCTTCACTCCAGTGTTGTCCCTACCATTTCGCAGCATTGAT
  TTAGGAGGCTCTGGGACAAAAGAGAAGCCAAAGAGCAGTTTTCCCAGTTCACTCACTCTG
  GCAAAATCAGGAAAAAAAAGTCTGCTTTTGACATCAAATTCCACTAATTTGGGGCAGCGT
  TGGGTGAGGAAAGTATTGTGAAGACAGGCTTCTTGGAGTAGGGGCAGCCACAATTCAGTA
  GACACTCTAGGCTCGGAGGCTGCCACTGTAGTTGCCAAGCTCAGGTTGGGTGGTTCTGTG
  CTGTATGGATGGAATAGGACCTGGGCTGGTCATCTTCATGTCGTTTCCTCTCTGTATCAA
  TGGAAGTTCAACCCGCCCCTACCTCTTCAGATAGTTGTAGGCCACTTTTCTCTTGTAACT
  TTGGAAAACAAAAGAGGAGAAATAAGTATCATACCATATGCGTGTCTCCAAAGTGGATGT
  GGTTGCCTCAAGGCAGGTGGCAGGCAGGGGTGACCTGCTGGCCCTCAGATCAATGGTCGT
  GGCAGGTCTGAGAGCTGTCCCACTGGCCAGACTTCTCTCCAGCAGCAAAGCCAGCCTGGG
  GCTTGCATGTTGATCCTGAGCAAGCTTAACGGGGTGAAGCTGGGCTTTCTCCCCCCTGTG
  ACTGGAGTGCATGTTGACACCAGCACTTTTTCTGCACATGTATCTTCAATCCAACAAGGC
  CGTTTTTTTAATGCTGAGTAACAGGCCACCAAGCGGCTACTGCGTTATATCTTCTCAGCA
  ACTGGCCGCAGTCTCTTCTGCACCATTTTCTACACCAGACCTGCTTGGCACCACAGGGAG
  CTCTTTTCCTGCCCTGCACAATGACATTCCAACCACCACCAGCCAGACATTACAGCCAAC
  CTTGCTGATTGTCACAAGCAGGACCTTGGGGCCACTGGCACTGTCAGATAGTAAGCCATT
  TCTTGGGTAGAGGAGGAAACTCCTCTCCACAAATCCACTTGGGCCTGTGCAAATGGCACT
  TGAAAGAGTCCCCATGCACTTGGAGTCCATGAGCCAATGGGATATGCAAAGACGCTTAAA
  CATTTCAGGGCTGGTTTCTCTGTTCATATCCAATTCTGGTGCTTAGGAACAGGGACCCAT
  GCTGATGCCCAAGGGCAAAAAGCCCCACTTCCTTTAAGGAAGTGAACAGGCCTGACCCTG
  ATGCCCAATAACGGGCAACCCTAGGCTTTTTGTTTTTCTTGCTTTTATTCCTTTTTGTTG
  TTGGCCTTGTGCTGCGTTTGTTTACAAAAGATGTATTTTGTTTAACCAAATATTAAAAAT
  GGAAAACTCCAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014876
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014876.3, NP_055691.1
• RefSeq Size: 3207 bp
• RefSeq ORF: 609 bp
• Locus ID: 9929
• UniProt ID: Q15040
• Cytogenetics: 22q13.1
• Domains: Josephin
• Protein Families: Druggable Genome
• Gene Summary: Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.