PSMD3 (NM_002809) Human Untagged Clone

CAT#: SC322282

PSMD3 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD3
• Synonyms: P58; RPN3; S3; TSTA2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322282 CCGGGAAGGGGTTTGCAGCTGCTCCGTCATCGTGCGGCCCGACGCTATCTCGCGCTCGTG
  TGCAGGCCCGGCTCGGCTCCTGGTCCCCGGTGCGAGGGTTAACGCGAGGCCCCGGCCTCG
  GTCCCCGGACTAGGCCGTGACCCCGGGTGCCATGAAGCAGGAGGGCTCGGCGCGGCGCCG
  CGGCGCGGACAAGGCGAAACCGCCGCCCGGCGGAGGAGAACAAGAACCCCCACCGCCGCC
  GGCCCCCCAGGATGTGGAGATGAAAGAGGAGGCAGCGACGGGTGGCGGGTCAACGGGGGA
  GGCAGACGGCAAGACGGCGGCGGCAGCGGCTGAGCACTCCCAGCGAGAGCTGGACACAGT
  CACCTTGGAGGACATCAAGGAGCACGTGAAACAGCTAGAGAAAGCGGTTTCAGGCAAGGA
  GCCGAGATTCGTGCTGCGGGCCCTGCGGATGCTGCCTTCCACATCACGCCGCCTCAACCA
  CTATGTTCTGTATAAGGCTGTGCAGGGCTTCTTCACTTCAAATAATGCCACTCGAGACTT
  TTTGCTCCCCTTCCTGGAAGAGCCCATGGACACAGAGGCTGATTTACAGTTCCGTCCCCG
  CACGGGAAAAGCTGCGTCGAACACCCTCCTGCCTGAAGTGGAAGCCTATCTCCAACTCCT
  CGTGGTCATCTTCATGATGAACAGCAAGCGCTACAAAGAGGCACAGAAGATCTCTGATGA
  TCTGATGCAGAAGATCAGTACTCAGAACCGCCGGGCCCTAGACCTTGTAGCCGCAAAGTG
  TTACTATTATCACGCCCGGGTCTATGAGTTCCTGGACAAGCTGGATGTGGTGCGCAGCTT
  CTTGCATGCTCGGCTCCGGACAGCTACGCTTCGGCATGACGCAGACGGGCAGGCCACCCT
  GTTGAACCTCCTGCTGCGGAATTACCTACACTACAGCTTGTACGACCAGGCTGAGAAGCT
  GGTGTCCAAGTCTGTGTTCCCAGAGCAGGCCAACAACAATGAGTGGGCCAGGTACCTCTA
  CTACACAGGGCGAATCAAAGCCATCCAGCTGGAGTACTCAGTGGCCCGGAGAACGATGAC
  CAACGCCCTTCGCAAGGCCCCTCAGCACACAGCTGTCGGCTTCAAACAGACGGTGCACAA
  GCTTCTCATCGTGGTGGAGCTGTTGCTGGGGGAGATCCCTGACCGGCTGCAGTTCCGCCA
  GCCCTCCCTCAAGCGCTCACTCATGCCCTATTTCCTTCTGACTCAAGCTGTCAGGACAGG
  AAACCTAGCCAAGTTCAACCAGGTCCTGGATCAGTTTGGGGAGAAGTTTCAAGCAGATGG
  GACCTACACCCTAATTATCCGGCTGCGGCACAACGTGATTAAGACAGGTGTACGCATGAT
  CAGCCTCTCCTATTCCCGAATCTCCTTGGCTGACATCGCCCAGAAGCTGCAGTTGGATAG
  CCCCGAAGATGCAGAGTTCATTGTTGCCAAGGCCATCCGGGATGGTGTCATTGAGGCCAG
  CATCAACCACGAGAAGGGCTATGTCCAATCCAAGGAGATGATTGACATCTATTCCACCCG
  AGAGCCCCAGCTAGCCTTCCACCAGCGCATCTCCTTCTGCCTAGATATCCACAACATGTC
  TGTCAAGGCCATGAGGTTTCCTCCCAAATCGTACAACAAGGACTTGGAGTCTGCAGAGGA
  ACGGCGTGAGCGAGAACAGCAGGACTTGGAGTTTGCCAAGGAGATGGCAGAAGATGATGA
  TGACAGCTTCCCTTGAGCTGGGGGGCTGGGGAGGGGTAGGGGGAATGGGGACAGGCTCTT
  TCCCCCTTGGGGGTCCCCTGCCCAGGGCACTGTCCCCATTTTCCCACACACAGCTCATAT
  GCTGCATTCGTGCAGGGGGTGGGGGTGCTGGGAGCCAGCCACCCTGACCTCCCCCAGGGC
  TCCTCCCCAGCCGGTGACTTACTGTACAGCAGGCAGGAGGGTGGGCAGGCAACCTCCCCG
  GGCAGGGTCCTGGCCAGCAGTGTGGGAGCAGGAGGGGAAGGATAGTTCTGTGTACTCCTT
  TAGGGAGTGGGGGACTAGAACTGGGATGTCTTGGCTTGTATGTTTTTTGAAGCTTCGATT
  ATGATTTTTAAACAATAAAAAGTTCTCCACAGTGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002809
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002809.2, NP_002800.2
• RefSeq Size: 2174 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5709
• UniProt ID: O43242
• Cytogenetics: 17q21.1
• Domains: PCI
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]