VPS37A (NM_152415) Human Untagged Clone

CAT#: SC322101

VPS37A (untagged)-Human vacuolar protein sorting 37 homolog A (S. cerevisiae) (VPS37A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS37A
• Synonyms: HCRP1; PQBP2; SPG53
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322101 TGTGGTCGGTTTGGGCGTCTGGGCCGTGAAGGTGGGACCTCCTGTTCCGGGCCGCAAGTT
  TCCCTCTCCAGCCGCCCGCCGTTCGTAGCATGTCCCCCAGAACTCGGGGAGCGCAGGCAG
  GACAGGCTTAGAGAAGACGCGGTCCCCAGCGCTTGGGCCACGGACGTCCCACCCCGCTCC
  TCTGTCGCTGGAGAACCGCCGGGCCGAGCCACTGGGAGAAGCAGGCCAGAGCCTTCCAGG
  GCCTCCGGCCCGTGGACCCGAGGAGGATGAGCTGGCTTTTTCCCCTGACCAAGAGCGCCT
  CCTCCTCCGCGGCTGGGTCCCCCGGTGGCCTCACCAGCCTCCAGCAGCAGAAGCAGCGCC
  TGATCGAGTCCCTCCGGAACTCACACTCCAGTATAGCCGAAATACAGAAAGATGTGGAAT
  ACAGATTGCCATTCACCATAAACAACCTGACAATTAACATTAATATACTGCTTCCTCCAC
  AGTTTCCTCAGGAAAAACCAGTGATCAGTGTTTATCCACCAATACGACATCACTTAATGG
  ATAAACAAGGAGTGTATGTTACCTCTCCATTAGTAAACAATTTTACAATGCACTCAGATC
  TTGGAAAAATTATTCAGAGTCTGTTGGATGAGTTTTGGAAGAATCCTCCAGTTTTAGCTC
  CTACTTCAACAGCATTTCCTTATCTATACAGTAACCCAAGTGGGATGTCTCCTTATGCTT
  CTCAGGGTTTTCCATTTCTTCCTCCATATCCTCCACAAGAAGCAAACAGGAGTATCACTT
  CTTTATCTGTTGCTGACACTGTTTCTTCTTCAACAACAAGTCATACCACAGCCAAGCCTG
  CCGCTCCTTCATTTGGTGTCCTTTCAAATCTGCCATTACCCATTCCCACAGTGGATGCTT
  CAATACCGACAAGCCAAAATGGTTTTGGGTACAAGATGCCAGATGTCCCTGATGCATTTC
  CAGAACTCTCAGAACTAAGTGTGTCACAACTCACAGATATGAATGAACAAGAGGAGGTAT
  TACTAGAACAGTTTCTGACTTTGCCTCAACTAAAACAAATTATTACCGACAAAGATGACT
  TAGTAAAAAGTATTGAGGAACTAGCAAGAAAAAATCTCCTTTTGGAGCCCAGCTTGGAAG
  CCAAAAGACAAACTGTTTTAGATAAGTATGAATTACTTACACAGATGAAGTCCACTTTCG
  AAAAGAAGATGCAAAGGCAGCATGAACTTAGTGAGATCTGTAGTGCAAGTGCCCTTCAGG
  CAAGATTGAAAGTAGCTGCACATGAAGCTGAGGAAGAATCTGATAATATTGCAGAAGATT
  TCTTGGAGGGAAAGATGGAAATAGATGATTTTCTCAGTAGCTTCATGGAAAAGAGAACAA
  TTTGCCACTGTAGAAGAGCCAAGGAAGAGAAACTTCAGCAGGCGATAGCAATGCACAGCC
  AATTTCATGCTCCACTATAGATTTTCCTGGAAACATGAACTGCCAAGAGAGGAATGGGAC
  ACAAAACCAAACACTGTTTTATATTTATGGTTTGCAAACTGGCATTTCATCAGTGGCTAA
  ATTCACAGATATCCTATATAGATTGTATACAGAACTGAGACTGATTTTGTACCGATTAGA
  ATGATTGCTATGATCTTTGAGAAATTTTTCTGCACTATTTGCACTGAAATGTTTATTTAT
  TGTTGATAAATTGTATCATATTTAAGTTCCACTGCTGTTCCTCTTACCTTGATTAAATGC
  CTATGCATGTACTTTTAGCTAGTTTTTAATATTTTATAAAACTTCATTTAAATTTGTATT
  TTTAACTTGAAGTTCCATTTCTTTATCAAGGATGGTATTTAGATTTTTTTCCTCTTAACC
  TTTTTTCAAAAACTATTTTCAACTGTGAGGAAACCCTTATTTTTCTTTCTTTGTGGATAA
  AACTTTCAAAAGCAATTTAAGATATTCATAGTGTTAGGAAACACCAAACCTGCCTATGTG
  CAATCTCACAAAAGAAACTTTTAATACCTACAATAAATCAAAAGAATAAACCAAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_152415
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152415.1, NP_689628.1
• RefSeq Size: 2020 bp
• RefSeq ORF: 1194 bp
• Locus ID: 137492
• UniProt ID: Q8NEZ2
• Cytogenetics: 8p22
• Protein Pathways: Endocytosis
• Gene Summary: This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.