Angiotensin II Type 1 Receptor (AGTR1) (NM_004835) Human Untagged Clone

CAT#: SC322091

AGTR1 (untagged)-Human angiotensin II receptor, type 1 (AGTR1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Angiotensin II Type 1 Receptor
• Synonyms: AG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322091 GATTCCAGCGCCTGACAGCCAGGACCCCAGGCAGCAGCGAGTGACAGGACGTCTGGACCG
  GCGCGCCGCTAGCAGCTCTGCCGGGCCGCGGCGGTGATCGATGGGGAGCGGCTGGAGCGG
  ACCCAGCGAGTGAGGGCGCACAGCCGGGACGCCGAGGCGGCGGGCGGGAGACCCGCACCA
  GCGCAGCCGGCCCTCGGCGGGACGTGACGCAGCGCCCGGGGCGCGGGTTTGATATTTGAC
  AAATTGATCTAAAATGGCTGGGTTTTTATCTGAATAACTCACTGATGCCATCCCAGAAAG
  TCGGCACCAGGTGTATTTGATATAGTGTTTGCAACAAATTCGACCCAGGTGATCAAAATG
  ATTCTCAACTCTTCTACTGAAGATGGTATTAAAAGAATCCAAGATGATTGTCCCAAAGCT
  GGAAGGCATAATTACATATTTGTCATGATTCCTACTTTATACAGTATCATCTTTGTGGTG
  GGAATATTTGGAAACAGCTTGGTGGTGATAGTCATTTACTTTTATATGAAGCTGAAGACT
  GTGGCCAGTGTTTTTCTTTTGAATTTAGCACTGGCTGACTTATGCTTTTTACTGACTTTG
  CCACTATGGGCTGTCTACACAGCTATGGAATACCGCTGGCCCTTTGGCAATTACCTATGT
  AAGATTGCTTCAGCCAGCGTCAGTTTCAACCTGTACGCTAGTGTGTTTCTACTCACGTGT
  CTCAGCATTGATCGATACCTGGCTATTGTTCACCCAATGAAGTCCCGCCTTCGACGCACA
  ATGCTTGTAGCCAAAGTCACCTGCATCATCATTTGGCTGCTGGCAGGCTTGGCCAGTTTG
  CCAGCTATAATCCATCGAAATGTATTTTTCATTGAGAACACCAATATTACAGTTTGTGCT
  TTCCATTATGAGTCCCAAAATTCAACCCTCCCGATAGGGCTGGGCCTGACCAAAAATATA
  CTGGGTTTCCTGTTTCCTTTTCTGATCATTCTTACAAGTTATACTCTTATTTGGAAGGCC
  CTAAAGAGGGCTTATGAAATTCAGAAGAACAAACCAAGAAATGATGATATTTTTAAGATA
  ATTATGGCAATTGTGCTTTTCTTTTTCTTTTCCTGGATTCCCCACCAAATATTCACTTTT
  CTGGATGTATTGATTCAACTAGGCATCATACGTGACTGTAGAATTGCAGATATTGTGGAC
  ACGGCCATGCCTATCACCATTTGTATAGCTTATTTTAACAATTGCCTGAATCCTCTTTTT
  TATGGCTTTCTGGGGAAAAAATTTAAAAGATATTTTCTCCAGCTTCTAAAATATATTCCC
  CCAAAAGCCAAATCCCACTCAAACCTTTCAACAAAAATGAGCACGCTTTCCTACCGCCCC
  TCAGATAATGTAAGCTCATCCACCAAGAAGCCTGCACCATGTTTTGAGGTTGAGTGACAT
  GTTCGAAACCTGTCCATAAAGTAATTTTGTGAAAGAAGGAGCAAGAGAACATTCCTCTGC
  AGCACTTCACTACCAAATGAGCCTTAGCTACTTTTCAGAATTGAAGGAGAAAATGCATTA
  TGTGGACTGAACCGACTTTTCTAAAGCTCTGAACAAAAGCTTTTCTTTCCTTTTGCAACA
  AGACAAAGCAAAGCCACATTTTGCATTAGACAGATGACGGCTGCTCGAAGAACAATGTCA
  GAAACTCGATGAATGTGTTGATTTGAGAAATTTTACTGACAGAAATGCAATCTCCCTAGC
  CTGCTTTTGTCCTGTTATTTTTTATTTCCACATAAAGGTATTTAGAATATATTAAATCGT
  TAGAGGAGCAACAGGAGATGAGAGTTCCAGATTGTTCTGTCCAGTTTCCAAAGGGCAGTA
  AAGTTTTCGTGCCGGTTTTCAGCTATTAGCAACTGTGCTACACTTGCACCTGGTACTGCA
  CATTTTGTACAAAGATATGCTAAGCAGTAGTCGTCAAGTTGCAGATCTTTTTGTGAAATT
  CAACCTGTGTCTTATAGGTTTACACTGCCAAAACAATGCCCGTAAGATGGCTTATTTGTA
  TAATGGTGTTACTAAAGTCACATATAAAAGTTAAACTACTTGTAAAGGTGCTGCACTGGT
  CCCAAGTAGTAGTGTCTTCCTAGTATATTAGTTTGATTTAATATCTGAGAAGTGTATATA
  GTTTGTGGTAAAAAGATTATATATCATAAAGTATGCCTTCCTGTTTAAAAAAAGTATATA
  TTCTACACATATATATATATGTATATCTATATCTCTAAACTGCTGTTAATTGATTAAAAT
  CTGGCAAAGTTATATTTACTTTAAAATAAAATAATTTTATTGCAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004835
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004835.3, NP_004826.2
• RefSeq Size: 2336 bp
• RefSeq ORF: 1080 bp
• Locus ID: 185
• UniProt ID: P30556
• Cytogenetics: 3q24
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Renin-angiotensin system, Vascular smooth muscle contraction
• Gene Summary: Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (3), also known as hAT1R-B (PubMed ID:11158334) or hAT1R-C (PubMed ID:16504375), has an alternate exon in the 5' region, which contains an in-frame AUG start codon, compared to variant 1. The resulting isoform (2) has a longer N-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.