GRHL3 (NM_198174) Human Untagged Clone

CAT#: SC321864

GRHL3 (untagged)-Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRHL3
• Synonyms: SOM; TFCP2L4; VWS2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198174.1 GGACGCGTGGGTGAGTGAGTAAACATCGGTGGATACCTGCCGCTCTCAGAGCGTGGAGGC
  TGGATGGGGTTTCCTGGAGGGGGAAGAGCGGGGAGGCCGGCACCGGGATTCACAGAGCAA
  TTTGTAAGGGTAAATGAATGGGCTGACGATCTTACTTTGGCGTGTCAAGGCAAGCCCCAG
  TCTCAAGCTAGAGTTTCAGGTCTGTGCGGCTGCTAAAGAACGACCCAGTCAACTTGCAGA
  AATTCTCTTACACTAGTGAGGATGAGGCCTGGAAGACGTACCTAGAAAACCCGTTGACAG
  CTGCCACAAAGGCCATGATGAGAGTCAATGGAGATGATGACAGTGTTGCGGCCTTGAGCT
  TCCTCTATGATTACTACATGGGTCCCAAGGAGAAGCGGATATTGTCCTCCAGCACTGGGG
  GCAGGAATGACCAAGGAAAGAGGTACTACCATGGCATGGAATATGAGACGGACCTCACTC
  CCCTTGAAAGCCCCACACACCTCATGAAATTCCTGACAGAGAACGTGTCTGGAACCCCAG
  AGTACCCAGATTTGCTCAAGAAGAATAACCTGATGAGCTTGGAGGGGGCCTTGCCCACCC
  CTGGCAAGGCAGCTCCCCTCCCTGCAGGCCCCAGCAAGCTGGAGGCCGGCTCTGTGGACA
  GCTACCTGTTACCCACCACTGATATGTATGATAATGGCTCCCTCAACTCCTTGTTTGAGA
  GCATTCATGGGGTGCCGCCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACC
  CACAGGAGTCGATGCTCTTCCCAGATATCCTGAAAACCTCCCCGGAACCCCCATGTCCAG
  AGGACTACCCCAGCCTCAAAAGTGACTTTGAATACACCCTGGGCTCCCCCAAAGCCATCC
  ACATCAAGTCAGGCGAGTCACCCATGGCCTACCTCAACAAAGGCCAGTTCTACCCCGTCA
  CCCTGCGGACCCCAGCAGGTGGCAAAGGCCTTGCCTTGTCCTCCAACAAAGTCAAGAGTG
  TGGTGATGGTTGTCTTCGACAATGAGAAGGTCCCAGTAGAGCAGCTGCGCTTCTGGAAGC
  ACTGGCATTCCCGGCAACCCACTGCCAAGCAGCGGGTCATTGACGTGGCTGACTGCAAAG
  AAAACTTCAACACTGTGGAGCACATTGAGGAGGTGGCCTATAATGCACTGTCCTTTGTGT
  GGAACGTGAATGAAGAGGCCAAGGTGTTCATCGGCGTAAACTGTCTGAGCACAGACTTTT
  CCTCACAAAAGGGGGTGAAGGGTGTCCCCCTGAACCTGCAGATTGACACCTATGACTGTG
  GCTTGGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAAGATCTTCTGTGACA
  AGGGAGCTGAGAGGAAGATGCGCGATGACGAGCGGAAGCAGTTCCGGAGGAAGGTCAAGT
  GCCCTGACTCCAGCAACAGTGGCGTCAAGGGCTGCCTGCTGTCGGGCTTCAGGGGCAATG
  AGACGACCTACCTTCGGCCAGAGACTGACCTGGAGACGCCACCCGTGCTGTTCATCCCCA
  ATGTGCACTTCTCCAGCCTGCAGCGCTCTGGAGGGGCAGCCCCCTCGGCAGGACCCAGCA
  GCTCCAACAGGCTGCCTCTGAAGCGTACCTGCTCGCCCTTCACTGAGGAGTTTGAGCCTC
  TGCCCTCCAAGCAGGCCAAGGAAGGCGACCTTCAGAGAGTTCTGCTGTATGTGCGGAGGG
  AGACTGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCAGACCTGAAGGGGCTGAGGA
  ATGCGATCTCTGAGAAGTATGGGTTCCCTGAAGAGAACATTTACAAAGTCTACAAGAAAT
  GCAAGCGAGGAATCTTAGTCAACATGGACAACAACATCATTCAGCATTACAGCAACCACG
  TCGCCTTCCTGCTGGACATGGGGGAGCTGGACGGCAAAATTCAGATCATCCTTAAGGAGC
  TGTAAGGCCTCTCGAGCATCCAAACCCTCACGACCTGCAAGGGGCCAGCAGGGACGTGGC
  CCCACGCCACACACAACCTCTCCACATGCCTCAGCGCTGTTACTTGAATGCCTTCCCTGA
  GGGAAGAGGCCCTTGAGTCACAGACCCACAGACGTCAGGGCCAGGGAGAGACCTAGGGGG
  TCCCCTGGCCTGGATCCCCATGGTATGCTTGAATCTGCTCCCTGAACTTCCTGCCAGTGC
  CTCCCCGTACCCCAAAACAATGTCACCATGGTTACCACCTACCCAGAAGACTGTTCCCTC
  CTCCCAAGACCCTTGTCTGCAGTGGTGCTCCTGCAGGCTGCCCGTTAAGATGGTGGCGGC
  ACACGCTCCCTCCCGCAGCACCACGCCAGCTGGTGCGGCCCCCACTCTCTGTCTTCCTTC
  AACTTCAGACAAAGGATTTCTCAACCTTTGGTCAGTTAACTTGAAAACTCTTGATTTTCA
  GTGCAAATGACTTTTAAAAGACACTATATTGGAGTCTCTTTCTCAGACTTCCTCAGCGCA
  GGATGTAAATAGCACTAACGATCGACTGGAACAAAGTGACCGCTGTGTAAAACTACTGCC
  TTGCCACTCACTGTTGTATACATTTCTTATTTACGATTTTCATTTGTTATATATATATAT
  AAATATACTGTATATATATGCAACATTTTATATTTTTCATGGATATGTTTTTATCATTTC
  AAAAAATGTGTATTTCACATTTCTTGGACTTTTTTTAGCTGTTATTCAGTGATGCATTTT
  GTATACTCACGTGGTATTTAGTAATAAAAATCTATCTATGTATTACGTCACATTAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_198174
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198174.1, NP_937817.2
• RefSeq Size: 2528 bp
• RefSeq ORF: 1530 bp
• Locus ID: 57822
• UniProt ID: Q8TE85
• Cytogenetics: 1p36.11
• Gene Summary: This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. This variant also differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has distinct N- and C-termini and is longer than isoform 1.