Epsin 1 (EPN1) (NM_013333) Human Untagged Clone

CAT#: SC321861

EPN1 (untagged)-Human epsin 1 (EPN1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Epsin 1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013333.2 CGGCGGCTCCCCTCCCCCGCCCGGCTCTCCGCGCCCCTTCTGGGCGGCGGGGCGGCGGAG
  CCGTCGGCGTGCGGCCCTCCTTGCGTTCGTGCGTGCGCCCGTGGCCCGGCGCACGTCCCG
  CGACACCGAGGCCGAGCGGGGCAGGGGGCTGACCGCCATGACCCCCCAGAGCCCGGCGTG
  AGGGGGCCGAGATGCGGTGACCTGCCAGCACCTGCCGCAGCCTTCGTCCGGGAGTCGCCC
  CATCTCTCCACGCATCGGGGCCCTGTGCCCCTTGCTGCTGCAGCCGGGCACCATGTCGAC
  CTCGTCCTTGAGGCGCCAGATGAAGAACATCGTCCACAACTACTCAGAGGCGGAGATCAA
  GGTTCGAGAGGCCACGAGCAATGACCCCTGGGGCCCATCCAGCTCCCTCATGTCAGAGAT
  TGCCGACCTCACCTACAACGTTGTCGCCTTCTCGGAGATCATGAGCATGATCTGGAAGCG
  GCTCAATGACCATGGCAAGAACTGGCGTCACGTTTACAAGGCCATGACGCTGATGGAGTA
  CCTCATCAAGACCGGCTCGGAGCGCGTGTCGCAGCAGTGCAAGGAGAACATGTACGCCGT
  GCAGACGCTGAAGGACTTCCAGTACGTGGACCGCGACGGCAAGGACCAGGGCGTGAACGT
  GCGTGAGAAAGCTAAGCAGCTGGTGGCCCTGCTGCGCGACGAGGACCGGCTGCGGGAAGA
  GCGGGCGCACGCGCTCAAGACCAAGGAAAAGCTGGCACAGACCGCCACGGCCTCATCAGC
  AGCTGTGGGCTCAGGCCCCCCTCCCGAGGCGGAGCAGGCGTGGCCGCAGAGCAGCGGGGA
  GGAGGAGCTGCAGCTCCAGCTGGCCCTGGCCATGAGCAAGGAGGAGGCCGACCAGGAGGA
  GCGGATCCGTCGCGGGGATGACCTGCGGCTGCAGATGGCAATCGAGGAGAGCAAGAGGGA
  GACTGGGGGCAAGGAGGAGTCGTCCCTCATGGACCTTGCTGACGTCTTCACGGCCCCAGC
  TCCTGCCCCGACCACAGACCCCTGGGGGGGCCCAGCACCCATGGCTGCTGCCGTCCCCAC
  GGCTGCCCCCACCTCGGACCCCTGGGGCGGCCCCCCTGTCCCTCCAGCTGCTGATCCCTG
  GGGAGGTCCAGCCCCCACGCCGGCCTCTGGGGACCCCTGGAGGCCTGCTGCCCCTGCAGG
  ACCCTCAGTTGACCCTTGGGGTGGGACCCCAGCCCCTGCAGCTGGGGAGGGGCCCACGCC
  TGATCCATGGGGAAGTTCCGATGGTGGGGTCCCGGTCAGTGGGCCCTCAGCCTCCGATCC
  CTGGACACCGGCCCCGGCCTTCTCAGATCCCTGGGGAGGGTCACCTGCCAAGCCCAGCAC
  CAATGGCACAACAGCCGGGGGATTCGACACGGAGCCCGACGAGTTCTCTGACTTTGACCG
  ACTCCGCACGGCACTGCCGACCTCCGGGAGCAGCGCAGGAGAGCTGGAGCTGCTGGCAGG
  AGAGGTGCCGGCCCGAAGCCCTGGGGCGTTTGACATGAGTGGGGTCAGGGGATCTCTGGC
  TGAGGCTGTGGGCAGCCCCCCACCTGCAGCCACACCAACTCCCACGCCCCCCACCCGGAA
  GACGCCGGAGTCATTCCTGGGGCCCAATGCAGCCCTCGTCGACCTGGACTCGCTGGTGAG
  CCGGCCGGGCCCCACGCCGCCTGGAGCCAAGGCCTCCAACCCCTTCCTGCCAGGCGGAGG
  CCCAGCCACTGGCCCTTCCGTCACCAACCCCTTCCAGCCCGCGCCTCCCGCGACGCTCAC
  CCTGAACCAGCTCCGTCTCAGTCCTGTGCCTCCCGTCCCTGGAGCGCCACCCACGTACAT
  CTCTCCCCTTGGCGGGGGCCCTGGCCTGCCCCCCATGATGCCCCCGGGCCCCCCGGCCCC
  CAACACTAATCCCTTCCTCCTATAATCCAGGGCGGAAGGGGGCCTGGCTCCATCCGGCTG
  CCCCATTCCGGCTCCCTGGGAGATCAGTGTTGTGAGTGCATGTGAAATGGGGGATCCCCA
  CCCCCAGTGCCCTTCCCCTTCCTGGGGCCCACTCACACTACACCCTCTTCCTTTCCCACC
  CCACCTCCCCGGAGAGAAACTGGACATGGGGCCTGGGGAGGGGAGCTGGCCAGAGGAGGA
  CCCCTTTCCCGTGGCATTAGAAGGGGGAGGGGTGGCTGGGGCCCCCACCCATTCCCCCTC
  CCTCCAAACTCCCAACCCCCAGTCAGTGTTTGAGCCTCCTCGTTCCCCTCACGCACCCGC
  TCACGCACCCTCGGTGAATCCTTGGTGATGATTTTGGCAACTTTGGGAATAAATGGCAAT
  TCCCACGGGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAG
• Restriction Sites: Please inquire
• ACCN: NM_013333
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013333.2, NP_037465.2
• RefSeq Size: 2430 bp
• RefSeq ORF: 1653 bp
• Locus ID: 29924
• UniProt ID: Q9Y6I3
• Cytogenetics: 19q13.42
• Domains: ENTH, UIM
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (c) is shorter at the N-terminus, compared to isoform a.