FBXO25 (NM_183421) Human Untagged Clone

CAT#: SC321837

FBXO25 (untagged)-Human F-box protein 25 (FBXO25), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXO25
• Synonyms: FBX25
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_183421.1 GGCAATAACCGCCTGGTCGCCGTCAGGTGCGGGCCCAGGTGGCCGGCGCGCCCGTTGGGC
  ACTGGGGGACGCGGGCGCGTCAGGTGAAGACTGGGGGCCGCAGGCGCGCTAGGAGAACTA
  TGCCATTTTTGGGTCAGGACTGGAGATCTCCTGGATGGAGTTGGATTAAGACAGAAGATG
  GCTGGAAGAGATGTGAATCTTGTAGTCAGAAACTTGAAAGAGAGAATAACCATTGTAACA
  TCAGTCACAGCATTATCTTAAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATG
  AATATGCATCGAAAAAAAGGAAAAAGGACCATTTTAGAAATGACACAAATACTCAAAGTT
  TTTATCGTGAAAAATGGATCTATGTCCATAAAGAAAGCACAAAGGAAAGGCATGGCTATT
  GCACCTTGGGAGAAGCCTTTAATCGGTTAGACTTCTCAAGTGCAATTCAAGATATCCGAA
  GGTTCAATTATGTGGTCAAACTGTTGCAGCTAATTGCAAAATCCCAGTTAACTTCATTGA
  GTGGCGTGGCACAGAAGAATTACTTCAACATTTTGGATAAAATCGTTCAAAAGGTTCTTG
  ATGACCACCACAATCCTCGCTTAATCAAAGATCTTCTGCAAGACCTAAGCTCTACCCTCT
  GCATTCTTATTAGAGGAGTAGGGAAGTCTGTATTAGTGGGAAACATCAATATTTGGATTT
  GCCGATTAGAAACTATTCTCGCCTGGCAACAACAGCTACAGGATCTTCAGATGACTAAGC
  AAGTGAACAATGGCCTCACCCTCAGTGACCTTCCTCTGCACATGCTGAACAACATCCTAT
  ACCGGTTCTCAGACGGATGGGACATCATCACCTTAGGCCAGGTGACCCCCACGTTGTATA
  TGCTTAGTGAAGACAGACAGCTGTGGAAGAAGCTTTGTCAGTACCATTTTGCTGAAAAGC
  AGTTTTGTAGACATTTGATCCTTTCAGAAAAAGGTCATATTGAATGGAAGTTGATGTACT
  TTGCACTTCAGAAACATTACCCAGCGAAGGAGCAGTACGGAGACACACTGCATTTCTGTC
  GGCACTGCAGCATTCTCTTTTGGAAGGACTACCATCTTGCTTTACTATTCAAGGACTCAG
  GACACCCCTGCACGGCGGCCGACCCTGACAGCTGCTTCACGCCTGTGTCTCCGCAGCACT
  TCATCGACCTCTTCAAGTTTTAAGGGCTGCCCCTGCCATCCCTATTGGAGATTGTGAATC
  CTGCTGTCTGTGCAGGGCTCATAGTGAGTGTTCTGTGAGGTGGGTGGAGACTCCTCGGAA
  GCCCCTGCTTCCAGAAAGCCTGGGAAGAACTGCCCTTCTGCAAAGGGGGGACTGCATGGT
  TGCATTTTCATCACTGAAAGTCAGAGGCCAAGGAAATCATTTCTACTTCTTTAAAAACTC
  CTTCTAAGCATATTAAAATGTGAAATTTTGCGTACTCTCTCTCTATATATATATAGTTCA
  AAAATACTTTAGGTGGTCAGCTCCACATTCTTTGTTGACGTGACACTAACGGCCAATAAT
  ATGCTTCTTAATTATCAAATTATAGTTTCCCAATTGGGAAACTAATTGGGGGTGGGTTAC
  AAAACATTTGATCCTTGTAAATACATTGTACAGAATATTTATTTTTTCTCAAAATGCATT
  TTAACTACTACATTGGCTGTGCCCAAATGAGTCCTCTTTGAATAGAAAGTGAACCCAGGG
  CAATGACAGCCATTCTTGTCTTAGGGATTATGGATCGGGGTATGAAGTGTGCACACGCAG
  CCCAACAACAGGCAGTGGTCTCTGTGCTCCTAGGCATCCAGCACAGGTTCTGGCAGGGCA
  CCCCTGCTGGGGTTGGGGGCTGGTCTGTGCATAATCCTGGACTGTGATGGGAACAGCCCA
  GTGCAGTCTAAACTTCAATTGTGTTGAAACTACTTTAATAGACAAAGTAATAAATCATGT
  TTATCTATTGATTTAAACTTCATCAGTTTTGCATCCTACTGAGAAATGTTAGTGATTTTG
  ATACTTAAATCCTTAAAAGATTGCTTCGTTTTTAAAATAACGCATGTCCATTTTAGAAAA
  TTAGAAAATCAGTCCCACCACCCAAAGATTATTGTGCATGCTGAAAAGAGTATGAAAAAT
  CCCCTCAGCAGGCATAGGATAGAAACGTATTGTTGTATATTTCCATTTTTGAATAGGGTC
  AAGGAGCCCAAGCAAATCATTTCTACTTTTTCCTTTAAGCATAATAATAAAAGTATACTT
  TTATGGCGTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_183421
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183421.1, NP_904357.1
• RefSeq Size: 2529 bp
• RefSeq ORF: 1104 bp
• Locus ID: 26260
• UniProt ID: Q8TCJ0
• Cytogenetics: 8p23.3
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1).