HDAC8 (NM_018486) Human Untagged Clone

CAT#: SC321829

HDAC8 (untagged)-Human histone deacetylase 8 (HDAC8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDAC8
• Synonyms: CDA07; CDLS5; HD8; HDACL1; KDAC8; MRXS6; RPD3; WTS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018486.1 GGAAGGTGGCTGCGGAACGGTTTTAAGCGGAAGATGGAGGAGCCGGAGGAACCGGCGGAC
  AGTGGGCAGTCGCTGGTCCCGGTTTATATCTATAGTCCCGAGTATGTCAGTATGTGTGAC
  TCCCCGGCCAAGATCCCCAAACGGGCCAGTATGGTGCATTCTTTGATTGAAGCATATGCA
  CTGCATAAGCAAATGAGGATAGTTAAGCCTAAAGTGGCCTCCATGGAGGAGATGGCCACC
  TTCCACACTGATGCTTATCTGCAGCATCTCCAGAAGGTCAGCCAAGAGGGCGATGATGAT
  CATCCGGACTCCATAGAATATGGGCTAGGTTATGACTGCCCAGCCACTGAAGGGATATTT
  GACTATGCAGCAGCTATAGGAGGGGCTACGATCACAGCTGCCCAATGCCTGATTGACGGA
  ATGTGCAAAGTAGCAATTAACTGGTCTGGAGGGTGGCATCATGCAAAGAAAGATGAAGCA
  TCTGGTTTTTGTTATCTCAATGATGCTGTCCTGGGAATATTACGATTGCGACGGAAATTT
  GAGCGTATTCTCTACGTGGATTTGGATCTGCACCATGGAGATGGTGTAGAAGACGCATTC
  AGTTTCACCTCCAAAGTCATGACCGTGTCCCTGCACAAATTCTCCCCAGGATTTTTCCCA
  GGAACAGGTGACGTGTCTGATGTTGGCCTAGGGAAGGGACGGTACTACAGTGTAAATGTG
  CCCATTCAGGATGGCATACAAGATGAAAAATATTACCAGATCTGTGAAAGTGTACTAAAG
  GAAGTATACCAAGCCTTTAATCCCAAAGCAGTGGTCTTACAGCTGGGAGCTGACACAATA
  GCTGGGGATCCCATGTGCTCCTTTAACATGACTCCAGTGGGAATTGGCAAGTGTCTTAAG
  TACATCCTTCAATGGCAGTTGGCAACACTCATTTTGGGAGGAGGAGGCTATAACCTTGCC
  AACACGGCTCGATGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACACTATCCTCT
  GAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACG
  CCAAGCTGCCGGCCAGACCGCAATGAGCCCCACCGAATCCAACAAATCCTCAACTACATC
  AAAGGGAATCTGAAGCATGTGGTCTAGTTGACAGAAAGAGATCAGGTTTCCAGAGCTGAG
  GAGTGGTGCCTATAATGAAGACAGCGTGTTTATGCAAGCAGTTTGTGGAATTTGTGACTG
  CAGGGAAAATTTGAAAGAAATTACTTCCTGAAAATTTCCAAGGGGCATCAAGTGGCAGCT
  GGCTTCCTGGGGTGAAGAGGCAGGCACCCCAGAGTCCTCAACTGGACCTAGGGGAAGAAG
  GAGATATCCCACATTTAAAGTTCTTATTTAAAAAAACACACACACACAAATGAAATTTTT
  AATCTTTGAAAATTATTTTTAAGCGAATTGGGGAGGGGAGTATTTTAATCATCTTAAATG
  AAACAGATCAGAAGCTGGATGAGAGCAGTCACCAGTTTGTAGGGCAGGAGGCAGCTGACA
  GGCAGGGTTTGGGCCTCAGGACCATCCAGGTGGAGCCCTGGGAGAGAGGGTACTGATCAG
  CAGACTGGGAGGTGGGGAGAAGTCCGCTGGTGTTGTTTTAGTGTTATATATCTTTGGTTT
  TTTTAATAAAATCTTTGAAAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_018486
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018486.1, NP_060956.1
• RefSeq Size: 1743 bp
• RefSeq ORF: 1134 bp
• Locus ID: 55869
• UniProt ID: Q9BY41
• Cytogenetics: Xq13.1
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: A downstream translational start codon is selected for this RefSeq based on a strong Kozak signal and transcript support. An upstream in-frame start codon is also present but has a weaker Kozak signal and sparse transcript support. Use of the upstream start codon would result in a protein that is 38 aa longer at the N-terminus. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon, which is supported by 5'RACE experiments described in PubMed: 10922473 and encodes a protein with an N-terminus similar to other family members.