PDLIM5 (NM_006457) Human Untagged Clone

CAT#: SC321417

PDLIM5 (untagged)-Human PDZ and LIM domain 5 (PDLIM5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDLIM5
• Synonyms: ENH; ENH1; L9; LIM
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006457.2 GGGACGGCGCCCTCACCGCGAGTCACTTGTCAGCCCTTGTCTGAGGCGGAGGCAGCCCCG
  CGCCGCGCCGGACCCGAGCATATTTCATTTTCTGTCATTGGACTTTGAGCCATTAGAACC
  ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGC
  GGTAAGGATTTCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCC
  CAGGCAAATGTAAGAATAGGCGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGA
  ATGACTCATCTTGAAGCCCAGAATAAGATTAAGGGTTGTACAGGCTCTTTGAATATGACT
  CTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCGGTTCCTGTTCAAAAGGGAGAACCT
  AAAGAAGTAGTTAAACCTGTGCCCATTACATCTCCTGCTGTGTCCAAAGTCACTTCCACA
  AACAACATGGCCTACAATAAGGCACCACGGCCTTTTGGTTCTGTGTCTTCACCAAAAGTC
  ACATCCATCCCATCACCATCGTCTGCCTTCACCCCAGCCCATGCGACCACCTCATCACAT
  GCTTCCCCTTCACCCGTGGCTGCCGTCACTCCTCCCCTGTTCGCTGCATCTGGACTGCAT
  GCTAATGCCAATCTTAGTGCTGACCAGTCTCCATCTGCACTGAGCGCTGGTAAAACTGCA
  GTTAATGTCCCACGGCAGCCCACAGTCACCAGCGTGTGTTCCGAGACTTCTCAGGAGCTA
  GCAGAGGGACAGAGAAGAGGATCCCAGGGTGACAGTAAACAGCAAAATGGCCCACCAAGA
  AAACACATTGTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAGTGATGCCAGC
  AAGAAGAGACTGATTGAGGATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCT
  CGCTCTTTCCGAATCCTTGCCCAGATCACTGGGACTGAACATTTGAAAGAATCTGAAGCC
  GATAATACAAAGAAGGCAAATAACTCTCAGGAGCCTTCTCCGCAGTTGGCTTCCTCGGTA
  GCTTCCACACGGAGCATGCCCGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGGGTT
  ACCAGCCTCACAACTGCAGCTGCCTTCAAGCCTGTAGGATCCACTGGCGTCATCAAGTCA
  CCAAGCTGGCAACGGCCAAACCAAGGAGTACCTTCCACTGGAAGAATCTCAAACAGCGCT
  GCTTACTCAGGATCAGTGGCACCAGCCAACTCAGCTTTGGGACAAACCCAGCCAAGTGAC
  CAGGACACTTTAGTGCAAAGAGCTGAGCACATTCCAGCAGGGAAACGAACTCCGATGTGC
  GCCCATTGTAACCAGGTCATCAGAGGACCATTCTTAGTGGCACTGGGGAAATCTTGGCAC
  CCAGAAGAATTCAACTGCGCTCACTGCAAAAATACAATGGCCTACATTGGATTTGTAGAG
  GAGAAAGGAGCCCTGTATTGTGAGCTGTGCTATGAGAAATTCTTTGCCCCTGAATGTGGT
  CGATGCCAAAGGAAGATCCTTGGAGAAGTCATCAATGCGTTGAAACAAACTTGGCATGTT
  TCCTGTTTTGTGTGTGTAGCCTGTGGAAAGCCCATTCGGAACAATGTTTTTCACTTGGAG
  GATGGTGAACCCTACTGTGAGACTGATTATTATGCCCTCTTTGGTACTATATGCCATGGA
  TGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGGAAGCTCTGGGCTACACCTGGCAT
  GACACTTGCTTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCAGACCTTTTTCTCC
  AAGAAGGACAAGCCCCTGTGTAAGAAACATGCTCATTCTGTGAATTTTTGAAAGTCAACA
  GTTCAGGAGAAGAGAAGGAATTTGAAGAGAAAAAGGAAAATTAAAATTACTAATTAATTT
  TTAGATTCAATATTTATATGGAGTTTTGAAAAATAATAGTGGCCCTGAAGGAATAAATTC
  CAGCTTTAAAAACCAAGTCTGAGGAAATATTTGGCTTCATAAAGTAAAGAGACGGTTTGG
  CATTTATTATTACTTTTTCCTGTATTTTATGCCCATAAAATAAGCTTTATAAAAACCAAT
  TTCCTGATGGACTATTAAATTCATCTTAGAATAAATTAGTGAAGAATTTAATTTTAGAAT
  AAATAATCCAATCTGAAATAATTATACCTTCTTTCCTTGTTAGGTAGTTATGAGTAAATC
  TGCAAAAGGCAATGAAAATGCCTTAAATTTTATCAATAACAGAATTATTGTATTTAAAAA
  AAAACTAATACTTATCTTTAAAATAGTAAATAGGATTTTAAACAGAGAATTTTATCAGTA
  ATAGGTGTCAGTTTTTAAAAAATTGCTTGTAGGCTGAGCGCGGTGGCTCACGCCTGTAAT
  CCCAGCTACTCAAGAGGCTGAGGCACGAGAATCACTTGAACCCGGGAGGGAGAGGTTGCA
  GTGAGCCAAGATCGTACCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCTGTCTCCA
  AAAAAAAACTTTGCTTGTATATTATTTTTGCCTTACAGTGGATCATTCTAGTAGGAAAGG
  ACAATAAGATTTTTTATCAAAATGTGTCATGCCAGTAAGAGATGTTATATTCTTTTCTCA
  TTTCTTCCCCACCCAAAAATAAGCTACCATATAGCTTATAAGTCTCAAATTTTTGCCTTT
  TACTAAAATGTGATTGTTTCTATTCATTGTGTATGCTTCATCACCTATATTAGGCAAATT
  CCATTTTTTTCCCTTGTGCTAAGGTAAAGATTTAATTAAATAATTTTGGCCTCTCATAGT
  TTTCTCTCTCTTTAAAGAGAATAAATAGAGGGCCAGGTGTGGTGGCTCACGCCTGTGATC
  CCAGCACTTTGGGAGGCCAAGACGGGCGGATCATGAGGTCAAGAGATCAAGATCATCCTG
  GCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATGAGCTGGGCATGGTGGGG
  CGTGCCTGTAGTCCCATGTACTTGGGAGGCTGAGGCAGGAAAATTCTTGAACCCAGGAGA
  CGGAAGTTGCAGTGAGCTGAGATCACACCACTGCACTCCAGCCTGGTGACAGAGCAAGAC
  TCCGGCTCTTAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006457
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006457.2, NP_006448.2
• RefSeq Size: 6101 bp
• RefSeq ORF: 1791 bp
• Locus ID: 10611
• UniProt ID: Q96HC4
• Cytogenetics: 4q22.3
• Domains: PDZ, LIM
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) encodes isoform a (also known as ENH1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.