E3 ubiquitin protein ligase MUL1 (MUL1) (NM_024544) Human Untagged Clone

CAT#: SC321149

MUL1 (untagged)-Human mitochondrial E3 ubiquitin protein ligase 1 (MUL1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E3 ubiquitin protein ligase MUL1
• Synonyms: C1orf166; GIDE; MAPL; MULAN; RNF218
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024544.1 GAGAGTCGGGGGTCACCAGGCCTATCCTTGGCGCCACAGTCGGCCACCGGGGCTCGCCGC
  CGTCATGGAGAGCGGAGGGCGGCCCTCGCTGTGCCAGTTCATCCTCCTGGGCACCACCTC
  TGTGGTCACCGCCGCCCTGTACTCCGTGTACCGGCAGAAGGCCCGGGTCTCCCAAGAGCT
  CAAGGGAGCTAAAAAAGTTCATTTGGGTGAAGATTTAAAGAGTATTCTTTCAGAAGCTCC
  AGGAAAATGCGTGCCTTATGCTGTTATAGAAGGAGCTGTGCGGTCTGTTAAAGAAACGCT
  TAACAGCCAGTTTGTGGAAAACTGCAAGGGGGTAATTCAGCGGCTGACACTTCAGGAGCA
  CAAGATGGTGTGGAATCGAACCACCCACCTTTGGAATGATTGCTCAAAGATCATTCATCA
  GAGGACCAACACAGTGCCCTTTGACCTGGTGCCCCACGAGGATGGCGTGGATGTGGCTGT
  GCGAGTGCTGAAGCCCCTGGACTCAGTGGATCTGGGTCTAGAGACTGTGTATGAGAAGTT
  CCACCCCTCGATTCAGTCCTTCACCGATGTCATCGGCCACTACATCAGCGGTGAGCGGCC
  CAAAGGCATCCAAGAGACCGAGGAGATGCTGAAGGTGGGGGCCACCCTCACAGGGGTTGG
  CGAACTGGTCCTGGACAACAACTCTGTCCGCCTGCAGCCGCCCAAACAAGGCATGCAGTA
  CTATCTAAGCAGCCAGGACTTCGACAGCCTGCTGCAGAGGCAGGAGTCGAGCGTCAGGCT
  CTGGAAGGTGCTGGCGCTGGTTTTTGGCTTTGCCACATGTGCCACCCTCTTCTTCATTCT
  CCGGAAGCAGTATCTGCAGCGGCAGGAGCGCCTGCGCCTCAAGCAGATGCAGGAGGAGTT
  CCAGGAGCATGAGGCCCAGCTGCTGAGCCGAGCCAAGCCTGAGGACAGGGAGAGTCTGAA
  GAGCGCCTGTGTAGTGTGTCTGAGCAGCTTCAAGTCCTGCGTCTTTCTGGAGTGTGGGCA
  CGTTTGTTCCTGCACCGAGTGCTACCGCGCCTTGCCAGAGCCCAAGAAGTGCCCTATCTG
  CAGACAGGCGATCACCCGGGTGATACCCCTGTACAACAGCTAATAGTTTGGAAGCCGCAC
  AGCTTGACCTGGAAGCACCCCTGCCCCCTTTTCAGGGATTTTTATCTCGAGGCCTTTGGA
  GGAGCAGTGGTGGGGGTAGCTGTCACCTCCAGGTATGATTGAGGGAGGAATTGGGTAGAA
  ACTCTCCAGACCCATGCCTCCAATGGCAGGATGCTGCCTTTCCCACCTGAGAGGGGACCC
  TGTCCATGTGCAGCCTCATCAGAGCCTCACCCTGGGAGGATGCCGTGGCGTCTCCTCCCA
  GGAGCCAGATCAGTGCGAGTGTGACTGAAAATGCCTCATCACTTAAGCACCAAAGCCAGT
  GATCAGCAGCTCTTCTGTTCCTGTGTCTTCTGTTTTTTTCTGGTGAATCGTTGCTTGCTG
  TGGACTTGGTGGAGGACTCAGAGGGGAGGAAAGGCTGGGCCCCGAGTACAACGGATGCCT
  TGGGTGCTGCCTCCGAAGAGACTCTGCCGCAGCTTTTCTTCTTTTTCCTCATGCCCCGGG
  AAACAGTCTTTCTTCAGAATTGTCAGGCTGGGCAGGTCAACTTGTGTTCCTTTCCCCTCA
  CCTGCTTGCCTCCTTAACGCCTGCACGTGTGTGTAGAGGACAAAAGAAAGTGAAGTCAGC
  ACATCCGCTTCTGCCCAGATGGTCGGGGCCCCGGGCAACAGATTGAAGAGAGATCATGTG
  AAGGGCAGTTGGTCAGGCAGGCCTCCTGGTTTCGCCACTGGCCCTGATTTGAACTCCTGC
  CACTTGGGAGAGCTCGGGGTGGTCCCTGGTTTTCCCTCCTGGAGAATGAGGCGCAGAGGC
  CTCGCCTCCTGAAGGACGCAGTGTGGATGCCACTGGCCTAGTGTCCTGGCCTCACAGCTT
  CCTTGCAAGGCTGTCACAAGGAAAAGCAGCCGGCTGGCACCCTGAGCATATGCCCTCTTG
  GGGCTCCCTCATCCAGCCCGTCGCAGCTTTGACATCTTGGTGTACTCATGTCGCTTCTCC
  TTGTGTTACCCCCTCCCAGTATTACCATTTGCCCCTCACCTGCCCTTGGTGAGCCTTTTA
  GTGCAAGACAGATGGGGCTGTTTTCCCCCACCTCTGAGTAGTTGGAGGTCACATACACAG
  CTCTTTTTTTTATTGCCCTTTTCTGCCTCTGAATGTTCATCTCTCGTCCTCCTTTGTGCA
  GGCGAGGAAGGGGTGCCCTCAGGGGCCGACACTAGTATGATGCAGTGTCCAGTGTGAACA
  GCAGAAATTAAACATGTTGCAACCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_024544
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024544.1, NP_078820.1
• RefSeq Size: 2442 bp
• RefSeq ORF: 1059 bp
• Locus ID: 79594
• UniProt ID: Q969V5
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Exhibits weak E3 ubiquitin-protein ligase activity (PubMed:18591963, PubMed:19407830, PubMed:22410793). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed:18591963, PubMed:19407830, PubMed:22410793). Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation (PubMed:22410793). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (PubMed:19407830). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial localization (PubMed:19407830, PubMed:18207745, PubMed:18213395). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed:24898855). May also be involved in the sumoylation of the membrane fission protein DNM1L (PubMed:18207745, PubMed:19407830). Inhibits cell growth (PubMed:18591963, PubMed:22410793). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis (PubMed:23399697). Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response (PubMed:23399697). Can mediate DDX58 sumoylation and disrupt its polyubiquitination (PubMed:23399697).[UniProtKB/Swiss-Prot Function]