Nucleoside Diphosphate Kinase 7 (NME7) (NM_197972) Human Untagged Clone

CAT#: SC321060

NME7 (untagged)-Human non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) (NME7), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Nucleoside Diphosphate Kinase 7
• Synonyms: CFAP67; MN23H7; NDK 7; NDK7; nm23-H7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_197972.1 GCCTGAGTAACCGTATGATGGTGGTGGTGGTGGTGTCTTCCTGTCTCAACGATACCTATT
  TTCTAGTGCTGAGATCCTGAGACAATGAATCATAGTGAAAGATTCGTTTTCATTGCAGAG
  TGGTATGATCCAAATGCTTCACTTCTTCGACGTTATGAGCTTTTATTTTACCCAGGGGAT
  GGATCTGTTGAAATGCATGATGTAAAGAATCATCGCACCTTTTTAAAGCGGACCAAATAT
  GATAACCTGCACTTGGAAGATTTATTTATAGGCAACAAAGTGAATGTCTTCTCTCGACAA
  CTGGTATTAATTGACTATGGGGATCAATATACAGCTCGCCAGCTGGGCAGTAGGAAAGAA
  AAAACGCTAGCCCTAATTAAACCAGATGCAATATCAAAGGCTGGAGAAATAATTGAAATA
  ATAAACAAAGCTGGATTTACTATAACCAAACTCAAAATGATGATGCTTTCAAGGAAAGAA
  GCATTGGATTTTCATGTAGATCACCAGTCAAGACCCTTTTTCAATGAGCTGATCCAGTTT
  ATTACAACTGGTCCTATTATTGCCATGGAGATTTTAAGAGATGATGCTATATGTGAATGG
  AAAAGACTGCTGGGACCTGCAAACTCTGGAGTGGCACGCACAGATGCTTCTGAAAGCATT
  AGAGCCCTCTTTGGAACAGATGGCATAAGAAATGCAGCGCATGGCCCTGATTCTTTTGCT
  TCTGCGGCCAGAGAAATGGAGTTGTTTTTTCCTTCAAGTGGAGGTTGTGGGCCGGCAAAC
  ACTGCTAAATTTACTAATTGTACCTGTTGCATTGTTAAACCCCATGCTGTCAGTGAAGGA
  CTGTTGGGAAAGATCCTGATGGCTATCCGAGATGCAGGTTTTGAAATCTCAGCTATGCAG
  ATGTTCAATATGGATCGGGTTAATGTTGAGGAATTCTATGAAGTTTATAAAGGAGTAGTG
  ACCGAATATCATGACATGGTGACAGAAATGTATTCTGGCCCTTGTGTAGCAATGGAGATT
  CAACAGAATAATGCTACAAAGACATTTCGAGAATTTTGTGGACCTGCTGATCCTGAAATT
  GCCCGGCATTTACGCCCTGGAACTCTCAGAGCAATCTTTGGTAAAACTAAGATCCAGAAT
  GCTGTTCACTGTACTGATCTGCCAGAGGATGGCCTATTAGAGGTTCAATACTTCTTCAAG
  ATCTTGGATAATTAGTGGTGTGGAAAGTAAAGAAGTCACAGGTTGGGACATTTAGACAAG
  AGTGAATCACACACGAGGAATGTGTTCATTCTTTTATTGTCCGTTGTTTTAACCTGACTG
  AATACAAGATCAACAAGAGCACTGTACTCCTGGCAATTATTACATATGTTAGAACATGGA
  TTTTGCACTGTAGACAACATTTAACACCAGTCTATGGGGTACTGCATTGCTTTTTATAAA
  GTTCAAAATAAAGATTTATTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_197972
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_197972.1, NP_932076.1
• RefSeq Size: 1615 bp
• RefSeq ORF: 1023 bp
• Locus ID: 29922
• UniProt ID: Q9Y5B8
• Cytogenetics: 1q24.2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes a member of the non-metastatic expressed family of nucleoside diphosphate kinases. Members of this family are enzymes that catalyzes phosphate transfer from nucleoside triphosphates to nucleoside diphosphates. This protein contains two kinase domains, one of which is involved in autophosphorylation and the other may be inactive. This protein localizes to the centrosome and functions as a component of the gamma-tubulin ring complex which plays a role in microtubule organization. Mutations in this gene may be associated with venous thromboembolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream AUG, compared to variant 1. The encoded protein (isoform b) is shorter than isoform a.