HGD (NM_000187) Human Untagged Clone

CAT#: SC321034

HGD (untagged)-Human homogentisate 1,2-dioxygenase (HGD)


  "NM_000187" in other vectors (5)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HGD
Synonyms AKU; HGO
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_000187.2 AATATCCAAAGAGTGGTTTGAAGGAGAAAGAAGCATTGTGGCTTTATATCCTCTGGGCCT
GGGTTTCCTGAAGTCACCACACATAGAGGAGAGAGAAAATGGCTGAGTTAAAGTACATTT
CTGGATTTGGGAATGAGTGTTCTTCAGAGGATCCTCGCTGCCCAGGTTCCCTGCCAGAAG
GACAGAATAATCCTCAGGTCTGCCCCTACAATCTCTATGCTGAGCAGCTCTCAGGATCGG
CTTTCACTTGTCCACGGAGCACCAATAAGAGAAGCTGGCTGTATAGGATTCTACCTTCAG
TTTCTCACAAGCCCTTTGAATCCATTGACGAAGGCCATGTCACTCACAACTGGGATGAAG
TTGATCCTGATCCTAACCAGCTTAGATGGAAACCATTTGAGATTCCAAAAGCATCTCAGA
AGAAAGTAGACTTTGTGAGTGGCCTGCATACCTTGTGTGGAGCTGGAGACATAAAGTCTA
ACAATGGGCTTGCTATCCACATTTTCCTCTGCAATACCTCCATGGAGAACAGATGCTTTT
ACAATTCAGATGGGGACTTCTTGATTGTTCCGCAGAAAGGGAACCTTCTCATTTACACCG
AGTTTGGCAAGATGCTTGTACAGCCCAATGAGATCTGCGTCATTCAGAGAGGAATGCGGT
TCAGCATAGATGTCTTTGAGGAGACCAGGGGCTACATCTTGGAGGTCTATGGTGTCCACT
TTGAGTTACCTGACCTTGGACCAATTGGGGCCAATGGCTTGGCCAATCCTCGTGATTTCT
TGATACCCATTGCCTGGTATGAGGATCGCCAAGTACCAGGTGGTTACACGGTCATTAATA
AATACCAGGGCAAGCTGTTTGCTGCCAAACAGGATGTCTCCCCGTTCAATGTTGTGGCCT
GGCACGGGAATTATACACCCTACAAGTACAACCTGAAGAATTTCATGGTTATCAACTCAG
TGGCCTTTGACCATGCAGACCCATCCATTTTCACAGTATTGACTGCTAAGTCTGTCCGCC
CTGGAGTGGCCATTGCTGATTTTGTCATCTTCCCACCTCGATGGGGGGTTGCTGATAAGA
CCTTCAGGCCTCCTTATTACCATAGGAACTGCATGAGTGAGTTCATGGGACTCATCCGAG
GTCACTATGAGGCAAAGCAAGGTGGGTTCCTGCCAGGGGGAGGGAGTCTACACAGCACAA
TGACCCCCCATGGACCTGATGCTGACTGCTTTGAGAAGGCCAGCAAGGTCAAGCTGGCAC
CTGAGAGGATTGCCGATGGCACCATGGCATTTATGTTTGAATCATCTTTAAGTCTGGCGG
TCACAAAGTGGGGACTCAAGGCCTCCAGGTGTTTGGATGAGAACTACCACAAGTGCTGGG
AGCCACTCAAGAGCCACTTCACTCCCAACTCCAGGAACCCAGCAGAACCTAATTGAGACT
GGAACATTGCTACCATAATTAAGAGTAGATTTGTGAAGATTTCTTCAGAATCTCATGCTT
TCTGGTAGTATTGGAGGAGGGGGTTGGTTAAAATGAAAATTCACTTTTCATAGTCAAGTA
ACTCAGAACTTTTATGGAAACGCATTTGCAAAGTTCTATGGCTGTCACCTTAATTACTCA
ATAAACTTGCTGGTGTTCTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000187
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000187.2, NP_000178.2
RefSeq Size 1920 bp
RefSeq ORF 1338 bp
Locus ID 3081
UniProt ID Q93099
Cytogenetics 3q13.33
Domains HgmA
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Tyrosine metabolism
Gene Summary This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]

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