BUD23 (NM_017528) Human Untagged Clone

CAT#: SC320894

WBSCR22 (untagged)-Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BUD23
• Synonyms: HASJ4442; HUSSY-3; MERM1; PP3381; WBMT; WBSCR22
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017528.2 GTGCTGCTGAGGCGTGAGAATGGCGTCCCGCGGCCGGCGTCCGGAGCATGGCGGACCCCC
  AGAGCTGTTTTATGACGAGACAGAAGCCCGGAAATACGTTCGCAACTCACGGATGATTGA
  TATCCAGACCAGGATGGCTGGGCGAGCATTGGAGCTTCTTTATCTGCCAGAGAATAAGCC
  CTGTTACCTGCTGGATATTGGCTGTGGCACTGGGCTGAGTGGAAGTTATCTGTCAGATGA
  AGGGCACTATTGGGTGGGCCTGGATATCAGCCCTGCCATGCTGGATGAGGCTGTGGACCG
  AGAGATAGAGGGAGACCTGCTGCTGGGGGATATGGGCCAGGGCATCCCATTCAAGCCAGG
  CACATTTGATGGTTGCATCAGCATTTCTGCTGTGCAGTGGCTCTGTAATGCTAACAAGAA
  GTCTGAAAACCCTGCCAAGCGCCTGTACTGCTTTTTTGCTTCTCTTTTTTCTGTTCTCGT
  CCGGGGATCCCGAGCTGTCCTGCAGCTGTACCCTGAGAACTCAGAGCAGTTGGAGCTGAT
  CACAACCCAGGCCACAAAGGCAGGCTTCTCCGGTGGCATGGTGGTAGACTACCCTAACAG
  TGCCAAAGCAAAGAAATTCTACCTCTGCTTGTTTTCTGGGCCTTCGACCTTTATACCAGA
  GGGGCTGAGTGAAAATCAGGATGAAGTTGAACCCAGGGAGTCTGTGTTCACCAATGAGAG
  GTTCCCATTAAGGATGTCGAGGCGGGGAATGGTGAGGAAGAGTCGGGCATGGGTGCTGGA
  GAAGAAGGAGCGGCACAGGCGCCAGGGCAGGGAAGTCAGACCTGACACCCAGTACACCGG
  CCGCAAGCGCAAGCCCCGCTTCTAAGTCACCACGCGGTTCTGGAAAGGCACTTGCCTCTG
  CACTTTTCTATATTGTTCAGCTGACAAAGTAGTATTTTAGAAAAGTTCTAAAGTTATAAA
  AATGTTTTCTGCAGTAAAAAAAAAGTTCTCTGGGCCGGGCGTGGTGGCTCACACCTGTAA
  TCCCAGCACCTTGGGAGGCTGAGGTGGGAGGATCATTTGAGGCCAGGAGTTTGAGACCTG
  CCTGGGCAACATAATGAAACTTCCTTTCCAGGGAGAAAAAAAAAAAAAAAAAAAAAAGCT
  CTGAGAGCATCTTATTTTGTTTAAAGGCAAGAAATAAAATTTCCTTTTGTGGAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_017528
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017528.2, NP_059998.2
• RefSeq Size: 1258 bp
• RefSeq ORF: 846 bp
• Locus ID: 114049
• UniProt ID: O43709
• Cytogenetics: 7q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism
• Gene Summary: This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.