PSMD2 (NM_002808) Human Untagged Clone

CAT#: SC320769

PSMD2 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD2
• Synonyms: P97; RPN1; S2; TRAP2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002808.3 ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCC
  CCCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGAC
  AAAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTG
  GAACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGG
  CAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGT
  CCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGT
  TTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC
  AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGG
  CATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAG
  CGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA
  GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGAC
  ATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTG
  CCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGC
  CGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC
  ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGG
  CATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC
  ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG
  GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGC
  AGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTT
  GTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTAC
  AAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG
  GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATT
  AAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGAC
  CCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT
  TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTG
  CTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTA
  GCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAG
  ACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT
  GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTG
  GAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATAT
  GCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT
  GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAG
  GAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATT
  GCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGA
  TATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA
  AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAA
  GTTTCCTATTACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGT
  CTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC
  ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC
  TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTT
  GTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGG
  CTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG
  CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAG
  ACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCA
  GAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT
  CGGAAGAACCCCAATTATGATCTCTAAGTGACCACCAGGGGCTCTGAACTGCAGCTGATG
  TTATCAGCAGGCCATGCATCCTGCTGCCAAGGGTGGACACGGCTGCAGACTTCTGGGGGA
  ATTGTCGCCTCCTGCTCTTTTGTTACTGAGTGAGATAAGGTTGTTCAATAAAGACTTTTA
  TCCCCAAGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002808
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002808.3, NP_002799.3
• RefSeq Size: 2990 bp
• RefSeq ORF: 2727 bp
• Locus ID: 5708
• UniProt ID: Q13200
• Cytogenetics: 3q27.1
• Domains: PC_rep
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).