HIF 2 alpha (EPAS1) (NM_001430) Human Untagged Clone

CAT#: SC320566

EPAS1 (untagged)-Human endothelial PAS domain protein 1 (EPAS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIF 2 alpha
• Synonyms: bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001430.3 CTTTTCCAGGGAAAAAGGAACTTGGGTTCCCTTCTCTCCGTCCTCTTTTCGGGTCTGACA
  GCCTCCACCCACTCCTTCCCCGGACCCCGCCTCCGCGCGCAGGTTCCTCCCAGTCACCTT
  TCTCCACCCCCGCCCCCGCACCTAGCCCGCCGCGCGCCACCTTCCACCTGACTGCGCGGG
  GCGCTCGGGACCTGCGCGCACCTCGGACCTTCACCACCCGCCCGGGCCGCGGGGAGCGGA
  CGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAGGTGCTCGGCGTCTGAACGTCTCAA
  AGGGCCACAGCGACAATGACAGCTGACAAGGAGAAGAAAAGGAGTAGCTCGGAGAGGAGG
  AAGGAGAAGTCCCGGGATGCTGCGCGGTGCCGGCGGAGCAAGGAGACGGAGGTGTTCTAT
  GAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTGAGCTCCCATCTGGACAAGGCCTCC
  ATCATGCGACTGGCAATCAGCTTCCTGCGAACACACAAGCTCCTCTCCTCAGTTTGCTCT
  GAAAACGAGTCCGAAGCCGAAGCTGACCAGCAGATGGACAACTTGTACCTGAAAGCCTTG
  GAGGGTTTCATTGCCGTGGTGACCCAAGATGGCGACATGATCTTTCTGTCAGAAAACATC
  AGCAAGTTCATGGGACTTACACAGGTGGAGCTAACAGGACATAGTATCTTTGACTTCACT
  CATCCCTGCGACCATGAGGAGATTCGTGAGAACCTGAGTCTCAAAAATGGCTCTGGTTTT
  GGGAAAAAAAGCAAAGACATGTCCACAGAGCGGGACTTCTTCATGAGGATGAAGTGCACG
  GTCACCAACAGAGGCCGTACTGTCAACCTCAAGTCAGCCACCTGGAAGGTCTTGCACTGC
  ACGGGCCAGGTGAAAGTCTACAACAACTGCCCTCCTCACAATAGTCTGTGTGGCTACAAG
  GAGCCCCTGCTGTCCTGCCTCATCATCATGTGTGAACCAATCCAGCACCCATCCCACATG
  GACATCCCCCTGGATAGCAAGACCTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACC
  TACTGTGATGACAGAATCACAGAACTGATTGGTTACCACCCTGAGGAGCTGCTTGGCCGC
  TCAGCCTATGAATTCTACCATGCGCTAGACTCCGAGAACATGACCAAGAGTCACCAGAAC
  TTGTGCACCAAGGGTCAGGTAGTAAGTGGCCAGTACCGGATGCTCGCAAAGCATGGGGGC
  TACGTGTGGCTGGAGACCCAGGGGACGGTCATCTACAACCCTCGCAACCTGCAGCCCCAG
  TGCATCATGTGTGTCAACTACGTCCTGAGTGAGATTGAGAAGAATGACGTGGTGTTCTCC
  ATGGACCAGACTGAATCCCTGTTCAAGCCCCACCTGATGGCCATGAACAGCATCTTTGAT
  AGCAGTGGCAAGGGGGCTGTGTCTGAGAAGAGTAACTTCCTATTCACCAAGCTAAAGGAG
  GAGCCCGAGGAGCTGGCCCAGCTGGCTCCCACCCCAGGAGACGCCATCATCTCTCTGGAT
  TTCGGGAATCAGAACTTCGAGGAGTCCTCAGCCTATGGCAAGGCCATCCTGCCCCCGAGC
  CAGCCATGGGCCACGGAGTTGAGGAGCCACAGCACCCAGAGCGAGGCTGGGAGCCTGCCT
  GCCTTCACCGTGCCCCAGGCAGCTGCCCCGGGCAGCACCACCCCCAGTGCCACCAGCAGC
  AGCAGCAGCTGCTCCACGCCCAATAGCCCTGAAGACTATTACACATCTTTGGATAACGAC
  CTGAAGATTGAAGTGATTGAGAAGCTCTTCGCCATGGACACAGAGGCCAAGGACCAATGC
  AGTACCCAGACGGATTTCAATGAGCTGGACTTGGAGACACTGGCACCCTATATCCCCATG
  GACGGGGAAGACTTCCAGCTAAGCCCCATCTGCCCCGAGGAGCGGCTCTTGGCGGAGAAC
  CCACAGTCCACCCCCCAGCACTGCTTCAGTGCCATGACAAACATCTTCCAGCCACTGGCC
  CCTGTAGCCCCGCACAGTCCCTTCCTCCTGGACAAGTTTCAGCAGCAGCTGGAGAGCAAG
  AAGACAGAGCCCGAGCACCGGCCCATGTCCTCCATCTTCTTTGATGCCGGAAGCAAAGCA
  TCCCTGCCACCGTGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCCATGGGGGGCAGATCC
  AATACCCAGTGGCCCCCAGATCCACCATTACATTTTGGGCCCACAAAGTGGGCCGTCGGG
  GATCAGCGCACAGAGTTCTTGGGAGCAGCGCCGTTGGGGCCCCCTGTCTCTCCACCCCAT
  GTCTCCACCTTCAAGACAAGGTCTGCAAAGGGTTTTGGGGCTCGAGGCCCAGACGTGCTG
  AGTCCGGCCATGGTAGCCCTCTCCAACAAGCTGAAGCTGAAGCGACAGCTGGAGTATGAA
  GAGCAAGCCTTCCAGGACCTGAGCGGGGGGGACCCACCTGGTGGCAGCACCTCACATTTG
  ATGTGGAAACGGATGAAGAACCTCAGGGGTGGGAGCTGCCCTTTGATGCCGGACAAGCCA
  CTGAGCGCAAATGTACCCAATGATAAGTTCACCCAAAACCCCATGAGGGGCCTGGGCCAT
  CCCCTGAGACATCTGCCGCTGCCACAGCCTCCATCTGCCATCAGTCCCGGGGAGAACAGC
  AAGAGCAGGTTCCCCCCACAGTGCTACGCCACCCAGTACCAGGACTACAGCCTGTCGTCA
  GCCCACAAGGTGTCAGGCATGGCAAGCCGGCTGCTCGGGCCCTCATTTGAGTCCTACCTG
  CTGCCCGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCTGGGAAGCTCCACG
  CTCCTGCAAGGAGGGGACCTCCTCAGAGCCCTGGACCAGGCCACCTGAGCCAGGCCTTCT
  ACCTGGGCAGCACCTCTGCCGACGCCGTCCCACCAGCTTCACTCTCTCCGTCTGTTTTTG
  CAACTAGGTATTTCTAACGCCAGCACACTATTTACAAGATGGACTTACCTGGCAGACTTG
  CCCAGGTCACCAAGCAGTGGCCTTTTTCTGAGATGCTCACTTTATTATCCCTATTTTTAA
  AGTACACAATTGTTTTACCTGTTCTGAAATGTTCTTAAATTTTGTAGGATTTTTTTCCTC
  CCCACCTTCAATGACTTCTAATTTATATTATCCATAGGTTTCTCTCCCTCCTTCTCCTTC
  TCACACACAACTGTCCATACTAACAAGTTTGGTGCATGTCTGTTCTTCTGTAGGGAGAAG
  CTTTAGCTTCATTTTACTAAAAAGATTCCTCGTTATTGTTGTTGCCAAAGAGAAACAAAA
  ATGATTTTGCTTTCCAAGCTTGGTTTGTGGCGTCTCCCTCGCAGAGCCCTTCTCGTTTCT
  TTTTTAAACTAATCACCATATTGTAAATTTCAGGGTTTTTTTTTTTGTTTAAGCTGACTC
  TTTGCTCTAATTTTGGAAAAAAAGAAATGTGAAGGGTCAACTCCAACGTATGTGGTTATC
  TGTGAAAGTTGCACAGCGTGGCTTTTCCTAAACTGGTGTTTTTCCCCCGCATTTGGTGGA
  TTTTTTATTATTATTCAAAAACATAACTGAGTTTTTTAAAAGAGGAGAAAATTTATATCT
  GGGTTAAGTGTTTATCATATATATGGGTACTTTGTAATATCTAAAAACTTAGAAACGGAA
  ATGGAATCCTGCTCACAAAATCACTTTAAGATCTTTTCGAAGCTGTTAATTTTTCTTAGT
  GTTGTGGACACTGCAGACTTGTCCAGTGCTCCCACGGCCTGTACGGACACTGTGGAAGGC
  CTCCCTCTGTCGGCTTTTTGCCATCTGTGATATGCCATAGGTGTGACAATCCGAGCAGTG
  GAGTCATTCAGCGGGAGCACTGCGCGCTATCCCCTCACATTCTCTATGTACTATGTATGT
  ATGTATTATTATTATTGCTGCCAAGAGGGTCTGATGGCACGTTGTGGGGTCGGGGGGTGG
  GGCGGGGAAGTGCTCTAACTTTTCTTAAGGTTTTGTTGCTAGCCCTTCAAGTGCACTGAG
  CTATGTGACTCGGATGGTCTTTCACACGGCACATTTGGACATTTCCAGAACTACCATGAG
  ATGGTTTAGACGGGAATTCATGCAAATGAGGGGTCAAAAATGGTATAGTGACCCCGTCCA
  CGTCCTCCAAGCTCACGACCTTGGAGCCCCGTGGAGCTGGACTGAGGAGGAGGCTGCACA
  GCGGGAGAGCAGCTGGTCCAGACCAGCCCTGCAGCCCCCACTCAGCCGGCAGCCAGATGG
  CCCCGCAAGGCCTCCAGGGATGGCCCCTAGCCACAGGCCCTGGCTGAGGTCTCTGGGTCG
  GTCAGTGACATGTAGGTAGGAAGCACTGAAAATAGTGTTCCCAGAGCACTTTGCAACTCC
  CTGGGTAAGAGGGACGACACCTCTGGTTTTTCAATACCAATTACATGGAACTTTTCTGTA
  ATGGGTACAATGAAGAAGTTTCTAAAAACACACACAAAGCACATTGGGCCAACTATTTAG
  TAAGCCCGGATAGACTTATTGCCAAAAACAAAAAATAGCTTTCAAAAGAAATTTAAGTTC
  TATGAGAAATTCCTTAGTCATGGTGTTGCGTAAATCATATTTTAGCTGCACGGCATTACC
  CCACACAGGGTGGCAGAACTTGAAGGGTTACTGACGTGTAAATGCTGGTATTTGATTTCC
  TGTGTGTGTTGCCCTGGCATTAAGGGCATTTTACCCTTGCAGTTTTACTAAAACACTGAA
  AAATATTCCAAGCTTCATATTAACCCTACCTGTCAACGTAACGATTTCATGAACGTTATT
  ATATTGTCGAATTCCTACTGACAACATTATAACTGTATGGGAGCTTAACTTTATAAGGAA
  ATGTATTTTGACACTGGTATCTTATTAAAGTATTCTGATCCTAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001430
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001430.3, NP_001421.2
• RefSeq Size: 5186 bp
• RefSeq ORF: 2613 bp
• Locus ID: 2034
• UniProt ID: Q99814
• Cytogenetics: 2p21
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]