RPP25 (NM_017793) Human Untagged Clone

CAT#: SC320291

RPP25 (untagged)-Human ribonuclease P/MRP 25kDa subunit (RPP25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPP25
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017793.2 GAGCGGCGCCCGCTCGAGAGAAGTGGGGCCGAGATCGCGCCGTCCCAGCCCGCCCGCCGG
  CGTCTGCGCTGCTCCGGCGCCCTTACCCCTGCTGGCCCTTGCAAGGCGCGACCGGCGGCC
  ATGCAGCCCCGGGGCTGAGGCCGCCCCATGGCAAAGCCGGCGGTCCCGGCGACGACGGCG
  CATGGAGAACTTCCGTAAGGTGCGCTCCGAAGAGGCGCCAGCGGGGTGCGGGGCCGAGGG
  AGGCGGCCCGGGCTCCGGCCCCTTCGCAGACCTGGCGCCGGGCGCGGTGCACATGCGGGT
  CAAGGAAGGCAGCAAGATCCGGAACCTGATGGCCTTCGCCACCGCCAGCATGGCGCAGCC
  AGCCACGCGCGCCATCGTCTTCAGCGGCTGCGGCCGGGCCACCACCAAAACCGTCACGTG
  CGCCGAGATCCTCAAGCGCCGCCTGGCGGGCCTGCACCAGGTCACGCGGCTGCGCTACCG
  GAGCGTACGCGAGGTGTGGCAGAGCCTCCCGCCTGGGCCCACGCAGGGTCAGACGCCTGG
  CGAGCCGGCCGCTAGTCTCAGCGTACTTAAGAACGTGCCCGGCCTCGCCATCCTACTTTC
  CAAGGACGCGCTGGATCCGCGACAGCCCGGCTACCAGCCCCCGAATCCCCATCCTGGTCC
  CTCGTCCCCGCCAGCCGCGCCAGCGTCCAAGAGGAGCCTAGGGGAACCCGCAGCTGGAGA
  AGGCTCCGCGAAGCGATCGCAACCCGAGCCAGGGGTTGCGGACGAGGATCAGACGGCCTG
  AAACTCCGGACTGTGGGCCACCTAGTCCGGCAGGATCTTCCGCCAGCGCCTCCACCTTCA
  GATGTACCTCCAGCCTTTCACTCCTCGGTCCTCCGGAGCCCACAACCTGGACACAGCTCC
  TCAAGACAGGTAGCATGGACCTTAAGGCGACAAGAGAGGGGCCCCAGGAGTGGCAGGAGA
  GGATCCCCGACCACCCTAGGGAAACTTCGTGCGATAATTGCTGCTTATGCCCGTGTTGCG
  TGAAGACACGGAACTTCACCTTTTCTGGGACCTTCTTTGGGGGAGAATGGTGGACAGTGG
  GATTCTGGAAACTTGTTTGGGACCCCCAAGGGTTAAAGCCACCCAGCATTCACGAAGAGC
  ACCTGAAGTATGGAAGCTGAGAAGAGTTATTGGAATCACCCCCACCGTTGACAGAGGAAG
  GCAGGGGGTGAGAATTAACTGCTTGAGGGTAGGAGAGTCTGAGATGTGGGGGCCCTATTC
  CGCTCCCCGCCCTGGGACAGTTTTCCTTTCTAGTTTTCTTTCCCCTGCTTCAGAGGAGCA
  TCTCTGAGGGTTCTAGCTCTTTTAACACCCCTTTTCCCCCTGCTGGCCCAGAGGGAGACC
  CAGGACTGAACTCTCCAGGCCTCCTCCCCTGAGCCCTGCAGCTGGCTGGAGTCTTGCTTC
  CAATAAAACAAGCACAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_017793
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017793.2, NP_060263.2
• RefSeq Size: 2333 bp
• RefSeq ORF: 600 bp
• Locus ID: 54913
• UniProt ID: Q9BUL9
• Cytogenetics: 15q24.2
• Gene Summary: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).[UniProtKB/Swiss-Prot Function]