GARS1 (NM_002047) Human Untagged Clone

CAT#: SC320151

GARS (untagged)-Human glycyl-tRNA synthetase (GARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GARS1
• Synonyms: CMT2D; DSMAV; GARS; GlyRS; HMN5; HMN5A; SMAD1; SMAJI
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002047.2 GCCGCTTCCGTCGCCACCCTCTCTGGACAGCCCAGGGCCGCAGGCTCATGCCCTCTCCGC
  GTCCAGTGCTGCTTAGAGGTGCTCGCGCCGCTCTGCTGCTGCTGCTGCCGCCCCGGCTCT
  TAGCCCGACCCTCGCTCCTGCTCCGCCGGTCCCTCAGCGCGGCCTCCTGCGCCCCGATCT
  CCTTGCCCGCCGCCGCCTCCCGGAGCAGCATGGACGGCGCGGGGGCTGAGGAGGTGCTGG
  CACCTCTGAGGCTAGCAGTGCGCCAGCAGGGAGATCTTGTGCGAAAACTCAAAGAAGATA
  AAGCACCCCAAGTAGACGTAGACAAAGCAGTGGCTGAGCTCAAAGCCCGCAAGAGGGTTC
  TGGAAGCAAAGGAGCTGGCGTTACAGCCCAAAGATGATATTGTAGACCGAGCAAAAATGG
  AAGATACCCTGAAGAGGAGGTTTTTCTATGATCAAGCTTTTGCTATTTATGGAGGTGTTA
  GTGGTCTGTATGACTTTGGGCCAGTTGGCTGTGCTTTGAAGAACAATATTATTCAGACCT
  GGAGGCAGCACTTTATCCAAGAGGAACAGATCCTGGAGATCGATTGCACCATGCTCACCC
  CTGAGCCAGTTTTAAAGACCTCTGGCCATGTAGACAAATTTGCTGACTTCATGGTGAAAG
  ACGTAAAAAATGGAGAATGTTTTCGTGCTGACCATCTATTAAAAGCTCATTTACAGAAAT
  TGATGTCTGATAAGAAGTGTTCTGTCGAAAAGAAATCAGAAATGGAAAGTGTTTTGGCCC
  AGCTTGATAACTATGGACAGCAAGAACTTGCGGATCTTTTTGTGAACTATAATGTAAAAT
  CTCCCATTACTGGAAATGATCTATCCCCTCCAGTGTCTTTTAACTTAATGTTCAAGACTT
  TCATTGGGCCTGGAGGAAACATGCCTGGGTACTTGAGACCAGAAACTGCACAGGGGATTT
  TCTTGAATTTCAAACGACTTTTGGAGTTCAACCAAGGAAAGTTGCCTTTTGCTGCTGCCC
  AGATTGGAAATTCTTTTAGAAATGAGATCTCCCCTCGATCTGGACTGATCAGAGTCAGAG
  AATTCACAATGGCAGAAATTGAGCACTTTGTAGATCCCAGTGAGAAAGACCACCCCAAGT
  TCCAGAATGTGGCAGACCTTCACCTTTATTTGTATTCAGCAAAAGCCCAGGTCAGCGGAC
  AGTCCGCTCGGAAAATGCGCCTGGGAGATGCTGTTGAACAGGGTGTGATTAATAACACAG
  TATTAGGCTATTTCATTGGCCGCATCTACCTCTACCTCACGAAGGTTGGAATATCTCCAG
  ATAAACTCCGCTTCCGGCAGCACATGGAGAATGAGATGGCCCATTATGCCTGTGACTGTT
  GGGATGCAGAATCCAAAACATCCTACGGTTGGATTGAGATTGTTGGATGTGCTGATCGTT
  CCTGTTATGACCTCTCCTGTCATGCACGAGCCACCAAAGTCCCACTTGTAGCTGAGAAAC
  CTCTGAAAGAACCCAAAACAGTCAATGTTGTTCAGTTTGAACCCAGTAAGGGAGCAATTG
  GTAAGGCATATAAGAAGGATGCAAAACTGGTGATGGAGTATCTTGCCATTTGTGATGAGT
  GCTACATTACAGAAATGGAGATGCTGCTGAATGAGAAAGGGGAATTCACAATTGAAACTG
  AAGGGAAAACATTTCAGTTAACAAAAGACATGATCAATGTGAAGAGATTCCAGAAAACAC
  TATATGTGGAAGAAGTTGTTCCGAATGTAATTGAACCTTCCTTCGGCCTGGGTAGGATCA
  TGTATACGGTATTTGAACATACATTCCATGTACGAGAAGGAGATGAACAGAGAACATTCT
  TCAGTTTCCCTGCTGTAGTTGCTCCATTCAAATGTTCCGTCCTCCCACTGAGCCAAAACC
  AGGAGTTCATGCCATTTGTCAAGGAATTATCGGAAGCCCTGACCAGGCATGGAGTATCTC
  ACAAAGTAGACGATTCCTCTGGGTCAATCGGAAGGCGCTATGCCAGGACTGATGAGATTG
  GCGTGGCTTTTGGTGTCACCATTGACTTTGACACAGTGAACAAGACCCCCCACACTGCAA
  CTCTGAGGGACCGTGACTCAATGCGGCAGATAAGAGCAGAGATCTCTGAGCTGCCCAGCA
  TAGTCCAAGACCTAGCCAATGGCAACATCACATGGGCTGATGTGGAGGCCAGGTATCCTC
  TGTTTGAAGGGCAAGAGACTGGTAAAAAAGAGACAATCGAGGAATGAGGACAATTTTGAC
  AACTTTTGACCACTTGCGCTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002047
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002047.2, NP_002038.2
• RefSeq Size: 2759 bp
• RefSeq ORF: 2220 bp
• Locus ID: 2617
• UniProt ID: P41250
• Cytogenetics: 7p14.3
• Domains: WHEP-TRS, tRNA-synt_2b, HGTP_anticodon
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).