KDEL Receptor (KDELR1) (NM_006801) Human Untagged Clone

CAT#: SC319596

KDELR1 (untagged)-Human KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 (KDELR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDEL Receptor
• Synonyms: ERD2; ERD2.1; HDEL; PM23
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006801.2 CTCTTCCCGGCTCCAGCTCCGCCGCCAGCTCCAGCCTTTGCTCCCCCTCCCAAAGTCCCC
  TCCCCGGAGCGGAGCGCACCTAGGGTCCCTCTTCCGTCCCCCCAGCCCAGCTACCCGTTC
  AGACCAGCAGCCTCGGGGGGCACCCCCCCGCCAGCCTGCCTCCCTCCCGCTCAGCCCTGC
  CAGGGTTCCCCAGCCATGAATCTCTTCCGATTCCTGGGAGACCTCTCCCACCTCCTCGCC
  ATCATCTTGCTACTGCTCAAAATCTGGAAGTCCCGCTCGTGCGCCGGAATTTCAGGGAAG
  AGCCAGGTCCTGTTTGCTGTGGTGTTCACTGCCCGATATCTGGACCTCTTCACCAACTAC
  ATCTCACTCTACAACACGTGTATGAAGGTGGTCTACATAGCCTGCTCCTTCACCACGGTC
  TGGTTGATTTATAGCAAGTTCAAAGCTACTTACGATGGGAACCATGACACGTTCAGAGTG
  GAGTTCCTGGTCGTTCCCACAGCCATTCTGGCGTTCCTGGTCAATCATGACTTCACCCCT
  CTGGAGATCCTCTGGACCTTCTCCATCTACCTGGAGTCAGTGGCCATCTTGCCGCAGCTG
  TTCATGGTGAGCAAGACCGGCGAGGCGGAGACCATCACCAGCCACTACTTGTTTGCGCTA
  GGCGTTTACCGCACGCTCTATCTCTTCAACTGGATCTGGCGCTACCATTTCGAGGGCTTC
  TTCGACCTCATCGCCATTGTGGCAGGCCTGGTCCAGACAGTCCTCTACTGCGATTTCTTC
  TACCTCTATATCACCAAAGTCCTAAAGGGGAAGAAGTTGAGTTTGCCGGCATAGCCCCGG
  TCCTCTCCATCTCTCTCCTCGGCAGCAGCGGGAGGCAGAGGAAGGCGGCAGAAGATGAAG
  AGCTTTCCCATCCAGGGGTGACTTTTTTAAGAACCCACCTCTTGTGCTCCCCATCCCGCC
  TCCTGCCGGGTTTCAGGGGGACAGTGGAGGATCCAGGTCTTGGGGAGCTCAGGACTTGGG
  CTGTTTGTAGTTTTTTGCCTTTTAGACAAGAAAAAAAAATCTTTCCACTCTTTAGTTTTT
  GATTCTGATGACTCGTTTTTCTTCTACTCTGTGGCCCCAATTTTTATAAAGTGTTTTTGA
  GTGTCCTATGGGCCGGGGCAGGGTCCAAGATCTTTTCCCTTCCCCAGGCCCCTCGGCTCC
  CTCCCAGATCCCACCCCCAGCCCCACTGGTTGCCAAACACTAAATCTGCCGACACCCATC
  TGCCCCACCTCCTGCCATGGCCATGAACCGCGACCCCCACTAAATTTCTAGATTGGGGAT
  AGGGAGAAAGGGAGGCCCAGGAAGGTCTCCCCTGATTTTTTTTCATAGTAATTTTTTTCC
  CCAGAGTTTGAATTTTTTGGTCTTCTCCTGGTTTTTTGGCAAATTAGGGGGGCCCGGGGC
  TCAAGTGCGGGAAGGGGGCTGGCCCGAGGATCCCATGGCTCTCACACCATGTTTTTGTAC
  AGAACTGATGGTTGAATCTTTGTTCTCTTGAAATAAACAGAAGAAAATGAAACCTTTAAA
  AAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006801
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006801.2, NP_006792.1
• RefSeq Size: 1575 bp
• RefSeq ORF: 639 bp
• Locus ID: 10945
• UniProt ID: P24390
• Cytogenetics: 19q13.33
• Domains: ER_lumen_recept
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• Gene Summary: Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]