LRRC8D (NM_018103) Human Untagged Clone

CAT#: SC319580

LRRC8D (untagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC8D
• Synonyms: LRRC5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018103.3 CTCTTGGGAGCGGGACTTCTGCTCAAATCCTGTCCAGGGGCTTGAAAAGGAGGAGAATTG
  GGGGTGGTTGCCGGGAGGACCAGCACCTCCAGTTACTGGAGGGTTGATAGGGGCCTTCTC
  CTTTCCGTCCCCCTTTACTAGGGGGTATCCCTTGCAGATAAATGAGATTTTTTGATTTCT
  TAAAAAGCCAGCTGCAGTGAAGAAGAACTGCTGCAGGTCGAGGGGAAGAGAGGAAGTGCA
  CGGCTGTCTATAACGTGCTGCCGGGTCTCAGGATGGAGGAGTGAAGTCTCCTGTCGCCGT
  GGTTCCAGCCTCCGGAGCTCGCCCAAGCCGCGTCCCCAGAGAGCGCCCTGAGAGAACAGG
  GTGGCCGCTTGGTCCAGGAATGTTTACCCTTGCGGAAGTTGCATCACTTAATGACATTCA
  GCCAACTTACCGAATCCTGAAACCATGGTGGGATGTGTTTATGGATTACCTAGCTGTTGT
  TATGTTAATGGTAGCCATCTTTGCAGGAACCATGCAACTTACCAAAGATCAGGTGGTCTG
  TTTGCCAGTATTGCCATCTCCTGTAAATTCAAAGGCACATACACCACCAGGAAATGCCGA
  GGTCACCACCAACATCCCAAAGATGGAAGCAGCCACCAACCAAGACCAAGATGGGCGGAC
  AACAAACGACATTTCCTTTGGGACATCTGCTGTGACACCTGACATACCTCTCAGAGCCAC
  ATATCCTCGCACAGATTTCGCACTTCCAAATCAGGAGGCAAAGAAAGAGAAGAAAGATCC
  AACAGGTCGAAAAACAAACTTGGATTTTCAGCAATATGTATTTATTAATCAAATGTGTTA
  CCATCTGGCCCTTCCGTGGTATTCTAAGTACTTTCCATACCTAGCTCTTATACATACTAT
  TATTCTCATGGTCAGTAGCAACTTTTGGTTCAAATATCCCAAAACATGCTCAAAAGTAGA
  ACATTTTGTTTCAATATTAGGAAAGTGCTTTGAATCCCCTTGGACGACAAAAGCGTTGTC
  TGAGACAGCATGCGAAGACTCAGAGGAAAACAAGCAGAGAATAACAGGTGCCCAGACTCT
  ACCAAAGCATGTTTCTACCAGCAGTGATGAAGGGAGCCCCAGTGCCAGTACACCAATGAT
  CAATAAAACTGGCTTTAAATTTTCAGCTGAGAAGCCTGTGATTGAAGTTCCCAGCATGAC
  AATCCTGGATAAAAAGGATGGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAAGTT
  CCGTGCCCATGTGGAAGATAGTGACTTGATCTATAAACTCTATGTGGTCCAAACAGTTAT
  CAAAACAGCCAAGTTCATTTTTATTCTCTGCTATACAGCGAACTTTGTCAACGCAATCAG
  CTTTGAACACGTCTGCAAGCCCAAAGTTGAGCATCTGATTGGTTATGAGGTATTTGAGTG
  CACCCACAATATGGCTTACATGTTGAAAAAGCTTCTCATCAGTTACATATCCATTATTTG
  TGTTTATGGCTTTATCTGCCTCTACACTCTCTTCTGGTTATTCAGGATACCTTTGAAGGA
  ATATTCTTTCGAAAAAGTCAGAGAAGAGAGCAGTTTTAGTGACATTCCAGATGTCAAAAA
  CGATTTTGCGTTCCTTCTTCACATGGTAGACCAGTATGACCAGCTATATTCCAAGCGTTT
  TGGTGTGTTCTTGTCAGAAGTTAGTGAAAATAAACTTAGGGAAATTAGTTTGAACCATGA
  GTGGACATTTGAAAAACTCAGGCAGCACATTTCACGCAACGCCCAGGACAAGCAGGAGTT
  GCATCTGTTCATGCTGTCGGGGGTGCCCGATGCTGTCTTTGACCTCACAGACCTGGATGT
  GCTAAAGCTTGAACTAATTCCAGAAGCTAAAATTCCTGCTAAGATTTCTCAAATGACTAA
  CCTCCAAGAGCTCCACCTCTGCCACTGCCCTGCAAAAGTTGAACAGACTGCTTTTAGCTT
  TCTTCGCGATCACTTGAGATGCCTTCACGTGAAGTTCACTGATGTGGCTGAAATTCCTGC
  CTGGGTGTATTTGCTCAAAAACCTTCGAGAGTTGTACTTAATAGGCAATTTGAACTCTGA
  AAACAATAAGATGATAGGACTTGAATCTCTCCGAGAGTTGCGGCACCTTAAGATTCTCCA
  CGTGAAGAGCAATTTGACCAAAGTTCCCTCCAACATTACAGATGTGGCTCCACATCTTAC
  AAAGTTAGTCATTCATAATGACGGCACTAAACTCTTGGTACTGAACAGCCTTAAGAAAAT
  GATGAATGTCGCTGAGCTGGAACTCCAGAACTGTGAGCTAGAGAGAATCCCACATGCTAT
  TTTCAGCCTCTCTAATTTACAGGAACTGGATTTAAAGTCCAATAACATTCGCACAATTGA
  GGAAATCATCAGTTTCCAGCATTTAAAACGACTGACTTGTTTAAAATTATGGCATAACAA
  AATTGTTACTATTCCTCCCTCTATTACCCATGTCAAAAACTTGGAGTCACTTTATTTCTC
  TAACAACAAGCTCGAATCCTTACCAGTGGCAGTATTTAGTTTACAGAAACTCAGATGCTT
  AGATGTGAGCTACAACAACATTTCAATGATTCCAATAGAAATAGGATTGCTTCAGAACCT
  GCAGCATTTGCATATCACTGGGAACAAAGTGGACATTCTGCCAAAACAATTGTTTAAATG
  CATAAAGTTGAGGACTTTGAATCTGGGACAGAACTGCATCACCTCACTCCCAGAGAAAGT
  TGGTCAGCTCTCCCAGCTCACTCAGCTGGAGCTGAAGGGGAACTGCTTGGACCGCCTGCC
  AGCCCAGCTGGGCCAGTGTCGGATGCTCAAGAAAAGCGGGCTTGTTGTGGAAGATCACCT
  TTTTGATACCCTGCCACTCGAAGTCAAAGAGGCATTGAATCAAGACATAAATATTCCCTT
  TGCAAATGGGATTTAAACTAAGATAATATATGCACAGTGATGTGCAGGAACAACTTCCTA
  GATTGCAAGTGCTCACGTACAAGTTATTACAAGATAATGCATTTTAGGAGTAGATACATC
  TTTTAAAATAAAACAGAGAGGATGCATAGAAGGCTGATAGAAGACATAACTGAATGTTCA
  ATGTTTGTAGGGTTTTAAGTCATTCATTTCCAAATCATTTTTTTTTTTCTTTTGGGGAAA
  GGGAAGGAAAAATTATAATCACTAATCTTGGTTCTTTTTAAATTGTTTGTAACTTGGATG
  CTGCCGCTACTGAATGTTTACAAATTGCTTGCCTGCTAAAGTAAATGATTAAATTGACAT
  TTTCTTACTATAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_018103
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018103.3, NP_060573.2
• RefSeq Size: 3329 bp
• RefSeq ORF: 2577 bp
• Locus ID: 55144
• UniProt ID: Q7L1W4
• Cytogenetics: 1p22.2
• Domains: LRR, LRR_TYP, LRR_BAC
• Protein Families: Transmembrane
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658, PubMed:28193731). Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24782309, PubMed:24790029, PubMed:26824658, PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Mediates the import of the antibiotic blasticidin-S into the cell (PubMed:24782309).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different segment for part of its 5' UTR, compared to variant 1. Variants 1 and 2 both encode the same protein.