ACTC1 (NM_005159) Human Untagged Clone

CAT#: SC319325

ACTC1 (untagged)-Human actin, alpha, cardiac muscle 1 (ACTC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTC1
• Synonyms: ACTC; ASD5; CMD1R; CMH11; LVNC4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005159.4 CCTCGGCAGTCCTAGCGGGTGCGAAGGGGACCAAATAAGGCAAGGTGGCAGACCGGGCCC
  CCCACCCCTGCCCCCGGCTGCTCCAACTGACCCTGTCCATCAGCGTTCTATAAAGCGGCC
  CTCCTGGAGCCAGCCACCCAGAGCCCGCTGCCGCCGGAGCCGAGCCGACCCGCCCCGCCG
  ACGAACCCCCTGAAGCTGTGCCAAGATGTGTGACGACGAGGAGACCACCGCCCTGGTGTG
  CGACAACGGCTCTGGGCTGGTGAAGGCCGGCTTTGCGGGCGATGACGCGCCCCGCGCTGT
  CTTCCCGTCCATCGTGGGCCGCCCGCGGCACCAGGGAGTTATGGTGGGTATGGGTCAGAA
  GGACTCCTACGTAGGTGATGAAGCCCAGAGCAAGAGAGGCATCCTGACCCTGAAGTATCC
  CATCGAGCATGGTATCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTT
  CTACAATGAGCTCCGTGTGGCTCCCGAGGAGCACCCCACCCTGCTCACAGAGGCCCCGCT
  GAACCCCAAGGCCAACCGGGAGAAGATGACTCAGATCATGTTTGAGACCTTCAATGTCCC
  TGCCATGTACGTGGCCATCCAGGCAGTGCTATCCCTGTATGCTTCTGGCCGTACCACAGG
  CATTGTTCTGGACTCTGGGGATGGTGTAACTCACAATGTCCCCATCTATGAGGGCTACGC
  TTTGCCCCATGCCATCATGCGTCTGGATCTGGCTGGTCGGGACCTCACTGACTACCTCAT
  GAAGATCCTCACTGAGCGTGGCTACTCCTTTGTCACCACTGCTGAACGTGAAATTGTCCG
  TGACATTAAAGAGAAGCTGTGCTATGTCGCCCTGGATTTTGAGAATGAGATGGCCACAGC
  TGCCTCTTCCTCCTCCCTGGAGAAGAGCTATGAACTGCCTGATGGCCAAGTCATCACTAT
  TGGCAATGAGCGCTTCCGCTGTCCTGAGACACTCTTCCAGCCCTCCTTCATTGGTATGGA
  ATCTGCTGGCATCCATGAAACAACTTACAATAGCATCATGAAGTGTGACATTGATATCCG
  CAAGGACCTGTATGCCAACAATGTCTTATCTGGAGGCACCACTATGTACCCTGGTATTGC
  TGATCGTATGCAGAAGGAAATCACTGCTCTGGCTCCTAGCACCATGAAGATTAAGATTAT
  TGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCTCTGTC
  CACCTTCCAGCAAATGTGGATTAGCAAGCAAGAGTACGATGAGGCAGGCCCATCCATTGT
  CCACCGCAAATGCTTCTAAGATGCCTTCTCTCTCCATCTACCTTCCAGTCAGGATGACGG
  TATTATGCTTCTTGGAGTCTTCCAAACCACCTTCCCTCATCTTTCATCAATCATTGTACA
  GTTTGTTTACACACGTGCAATTTGTTTGTGCTTCTAATATTTATTGCTTTATAAATAAAC
  CAGACCAGGACTTGCAACCTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005159
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005159.4, NP_005150.1
• RefSeq Size: 3693 bp
• RefSeq ORF: 1134 bp
• Locus ID: 70
• UniProt ID: P68032
• Cytogenetics: 15q14
• Domains: ACTIN
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]