Glutathione Synthetase (GSS) (NM_000178) Human Untagged Clone

CAT#: SC319136

GSS (untagged)-Human glutathione synthetase (GSS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutathione Synthetase
• Synonyms: GSHS; HEL-S-64p; HEL-S-88n
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000178.2 ACGAGGGAGGCCCCGCCCCCTGAGCCTGGGTAGCGGCGCGAGGGCCGGGAGAACCGTTCG
  CGGAGGAAAGGCGAACTAGTGTTGGGATGGCCACCAACTGGGGGAGCCTCTTGCAGGATA
  AACAGCAGCTAGAGGAGCTGGCACGGCAGGCCGTGGACCGGGCCCTGGCTGAGGGAGTAT
  TGCTGAGGACCTCACAGGAGCCCACTTCCTCGGAGGTGGTGAGCTATGCCCCATTCACGC
  TCTTCCCCTCACTGGTCCCCAGTGCCCTGCTGGAGCAAGCCTATGCTGTGCAGATGGACT
  TCAACCTGCTAGTGGATGCTGTCAGCCAGAACGCTGCCTTCCTGGAGCAAACTCTTTCCA
  GCACCATCAAACAGGATGACTTTACCGCTCGTCTCTTTGACATCCACAAGCAAGTCCTAA
  AAGAGGGCATTGCCCAGACTGTGTTCCTGGGCCTGAATCGCTCAGACTACATGTTCCAGC
  GCAGCGCAGATGGCTCCCCAGCCCTGAAACAGATCGAAATCAACACCATCTCTGCCAGCT
  TTGGGGGCCTGGCCTCCCGGACCCCAGCTGTGCACCGACATGTTCTCAGTGTCCTGAGTA
  AGACCAAAGAAGCTGGCAAGATCCTCTCTAATAATCCCAGCAAGGGACTGGCCCTGGGAA
  TTGCCAAAGCCTGGGAGCTCTACGGCTCACCCAATGCTCTGGTGCTACTGATTGCTCAAG
  AGAAGGAAAGAAACATATTTGACCAGCGTGCCATAGAGAATGAGCTACTGGCCAGGAACA
  TCCATGTGATCCGACGAACATTTGAAGATATCTCTGAAAAGGGGTCTCTGGACCAAGACC
  GAAGGCTGTTTGTGGATGGCCAGGAAATTGCTGTGGTTTACTTCCGGGATGGCTACATGC
  CTCGTCAGTACAGTCTACAGAATTGGGAAGCACGTCTACTGCTGGAGAGGTCACATGCTG
  CCAAGTGCCCAGACATTGCCACCCAGCTGGCTGGGACTAAGAAGGTGCAGCAGGAGCTAA
  GCAGGCCGGGCATGCTGGAGATGTTGCTCCCTGGCCAGCCTGAGGCTGTGGCCCGCCTCC
  GCGCCACCTTTGCTGGCCTCTACTCACTGGATGTGGGTGAAGAAGGGGACCAGGCCATCG
  CCGAGGCCCTTGCTGCCCCTAGCCGGTTTGTGCTAAAGCCCCAGAGAGAGGGTGGAGGTA
  ACAACCTATATGGGGAGGAAATGGTACAGGCCCTGAAACAGCTGAAGGACAGTGAGGAGA
  GGGCCTCCTACATCCTCATGGAGAAGATCGAACCTGAGCCTTTTGAGAATTGCCTGCTAC
  GGCCTGGCAGCCCTGCCCGAGTGGTCCAGTGCATTTCAGAGCTGGGCATCTTTGGGGTCT
  ATGTCAGGCAGGAAAAGACACTCGTGATGAACAAGCACGTGGGGCATCTACTTCGAACCA
  AAGCCATCGAGCATGCAGATGGTGGTGTGGCAGCGGGAGTGGCAGTCCTGGACAACCCAT
  ACCCTGTGTGAGGGCACAACCAGGCCACGGGACCTTCTATCCTCTGTATTTGTCATTCCT
  CTCCTAGCCCTCCTGAGGGGTATCCTCCTAAAGACCTCCAAAGTTTTTATGGAAGGGTAA
  ATACTGGTACCTTCCCCCAGCTTTCCATCTGAGGACCAGAAAAGTTGTGTCTCCCTTAGA
  TGAGATCTAGACGCCCCCAAATCCTTGAGATGTGGGTATAGCTCAGGGTAAGCTGCTCTG
  AGGTAAAGGTCCATGAACCCTGCCCCACTCCTGTCAGCCCCTCATCAGCCTTTTCAGCAG
  GTTCCAGTGCCTGACTTGGGATAGGACTGAGTGGTAGGAGGAGGGGGAGTGGAGGGGCAT
  AGCCTTTCCCTAATTCTGCCTTAAATAAAACTGCATTGCTGATTCAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_000178
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000178.2, NP_000169.1
• RefSeq Size: 1918 bp
• RefSeq ORF: 1425 bp
• Locus ID: 2937
• UniProt ID: P48637
• Cytogenetics: 20q11.22
• Domains: GSH_synthase, GSH_synth_ATP
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways
• Gene Summary: Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]