CHD2 (NM_001271) Human Untagged Clone

CAT#: SC318677

CHD2 (untagged)-Human chromodomain helicase DNA binding protein 2 (CHD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHD2
• Synonyms: DEE94; EEOC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001271, the custom clone sequence may differ by one or more nucleotides
  ATGATGAGAAATAAGGACAAAAGCCAAGAGGAGGACAGTTCGCTACACAGCAATGCATCG
  AGTCACTCAGCCTCTGAAGAAGCTTCGGGTTCAGACTCAGGCAGTCAGTCGGAAAGTGAG
  CAGGGAAGTGATCCAGGAAGTGGACATGGCAGCGAGTCGAACAGCAGCTCTGAATCTTCT
  GAGAGTCAGTCGGAATCTGAGAGCGAATCAGCAGGTTCCAAATCCCAGCCAGTCCTCCCA
  GAAGCCAAAGAGAAGCCAGCCTCTAAGAAGGAACGGATAGCTGATGTGAAGAAGATGTGG
  GAAGAATATCCTGATGTTTATGGGGTCAGGCGGTCAAACCGAAGCAGACAAGAACCATCG
  CGATTTAATATTAAGGAAGAGGCAAGTAGCGGGTCTGAGAGTGGGAGCCCAAAAAGAAGA
  GGCCAGAGGCAGCTGAAAAAACAAGAAAAATGGAAACAGGAACCCTCAGAAGATGAACAG
  GAACAAGGCACCAGTGCAGAGAGTGAGCCAGAACAAAAAAAAGTAAAAGCCAGAAGACCT
  GTCCCCAGAAGAACAGTGCCCAAACCTCGTGTTAAAAAGCAGCCGAAGACTCAGCGTGGA
  AAGAGAAAAAAGCAAGATTCTTCTGATGAGGATGATGATGATGACGAAGCTCCCAAAAGG
  CAGACTCGTCGAAGAGCGGCTAAAAACGTTAGTTACAAAGAAGATGATGACTTTGAGACT
  GACTCAGATGATCTCATTGAAATGACTGGAGAAGGAGTTGATGAACAGCAAGATAATAGT
  GAAACTATTGAAAAGGTCTTAGATTCAAGACTGGGAAAGAAAGGAGCCACTGGAGCATCT
  ACTACTGTATATGCGATTGAAGCTAATGGCGACCCTAGTGGTGACTTTGACACTGAAAAG
  GATGAAGGTGAAATCCAGTACCTCATCAAGTGGAAGGGTTGGTCTTACATCCACAGCACA
  TGGGAGAGTGAAGAATCCTTACAGCAACAGAAAGTGAAGGGCCTAAAAAAACTAGAGAAC
  TTCAAGAAAAAAGAGGACGAAATCAAACAATGGTTAGGGAAAGTTTCTCCTGAAGATGTA
  GAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGAATAAACAGTATCAGATAGTA
  GAAAGAGTAATAGCTGTGAAGACAAGTAAATCTACATTGGGTCAAACAGATTTTCCAGCT
  CATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGTAAATGGATGGGACTC
  CCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCCTCATTGGAAAGAAATTCCAGAATTGC
  ATTGACAGCTTCCACAGTAGGAACAACTCAAAAACCATCCCAACAAGAGAATGCAAGGCC
  CTGAAGCAGAGACCACGATTTGTAGCTTTAAAGAAACAACCTGCATATTTAGGAGGGGAG
  AATCTGGAACTTCGAGATTATCAGCTAGAAGGTCTAAACTGGCTAGCTCATTCCTGGTGC
  AAAAATAATAGTGTAATCCTTGCTGATGAAATGGGCCTAGGAAAGACCATCCAGACCATA
  TCATTCCTCTCCTACCTGTTCCACCAACACCAGCTGTATGGCCCCTTTCTTATAGTCGTC
  CCTTTATCCACCCTCACCTCATGGCAGAGAGAGTTTGAAATCTGGGCACCAGAGATTAAC
  GTAGTGGTTTACATAGGTGACCTGATGAGCAGAAATACGATACGGGAATATGAATGGATT
  CATTCCCAAACCAAAAGATTGAAGTTCAACGCACTTATAACAACATATGAGATCCTCTTG
  AAAGATAAGACTGTGCTGGGCAGTATTAACTGGGCCTTTCTGGGAGTGGATGAAGCCCAT
  CGGTTGAAGAATGATGACTCTTTATTGTATAAAACTCTGATTGATTTCAAGTCCAACCAT
  AGGCTCCTGATTACGGGGACCCCTCTTCAGAATTCCCTCAAAGAGCTCTGGTCCTTGCTG
  CACTTTATTATGCCGGAGAAGTTTGAATTTTGGGAAGATTTTGAAGAAGACCATGGGAAG
  GGGAGAGAAAATGGCTACCAGAGTCTTCATAAGGTGCTAGAGCCTTTCCTTCTCCGGAGA
  GTCAAAAAAGATGTGGAGAAATCCCTTCCTGCTAAAGTGGAACAGATTCTCAGGGTGGAG
  ATGTCAGCCCTTCAGAAACAGTATTACAAGTGGATTCTGACCAGGAATTACAAGGCTCTT
  GCCAAAGGAACAAGAGGCAGCACATCTGGTTTTCTTAATATTGTGATGGAACTGAAAAAA
  TGTTGCAACCACTGCTATCTGATTAAACCCCCTGAAGAAAATGAAAGGGAAAATGGACAG
  GAGATTCTTCTGTCCCTCATAAGGAGCAGTGGGAAGTTGATTTTATTAGACAAACTGTTG
  ACAAGACTTCGAGAAAGGGGGAATCGAGTGCTTATCTTCTCTCAGATGGTGAGAATGTTG
  GATATCCTGGCTGAATACCTAACTATTAAACACTATCCTTTCCAGCGTCTGGATGGTTCC
  ATCAAGGGAGAAATCCGAAAACAGGCACTGGACCACTTCAATGCAGATGGGTCTGAGGAC
  TTCTGTTTCCTGCTCTCGACAAGGGCTGGTGGCCTGGGAATCAATTTGGCTTCAGCGGAC
  ACAGTCGTCATCTTTGACTCTGACTGGAACCCCCAGAATGACTTGCAGGCACAAGCCCGA
  GCGCATAGAATTGGTCAAAAGAAGCAGGTAAATATTTACCGCTTAGTTACAAAGGGGACT
  GTGGAGGAGGAGATCATAGAACGGGCCAAAAAGAAGATGGTATTAGATCATCTGGTGATT
  CAGCGCATGGACACCACTGGCCGGACGATCCTGGAAAACAACTCAGGAAGGTCCAACTCA
  AATCCTTTTAATAAAGAAGAGCTGACAGCTATTTTGAAATTTGGAGCAGAGGATCTCTTC
  AAAGAACTGGAAGGGGAGGAATCAGAACCTCAGGAAATGGATATAGATGAAATTTTGCGG
  TTGGCTGAAACGAGAGAGAATGAAGTGTCAACAAGTGCAACAGATGAACTTCTATCACAG
  TTTAAGGTTGCCAACTTTGCAACAATGGAAGATGAAGAAGAGCTAGAAGAGCGTCCTCAC
  AAGGACTGGGATGAGATCATTCCAGAGGAACAAAGGAAAAAAGTAGAGGAGGAAGAGCGG
  CAGAAGGAGCTAGAAGAAATTTATATGCTGCCTCGAATTCGGAGTTCCACTAAAAAGGCT
  CAGACAAATGACAGTGACTCTGACACTGAGTCTAAGAGGCAGGCCCAGAGATCCTCTGCT
  TCTGAGAGTGAAACGGAAGACTCTGATGATGACAAGAAGCCAAAGCGCAGAGGGCGTCCG
  AGGAGTGTGCGGAAGGACCTCGTGGAGGGATTTACTGATGCAGAGATCCGAAGGTTCATC
  AAGGCTTATAAGAAGTTTGGTCTCCCTCTTGAACGGCTGGAGTGCATAGCACGTGATGCT
  GAGCTGGTAGATAAGTCGGTGGCAGATCTGAAGCGCCTGGGTGAACTGATCCACAACAGC
  TGTGTGTCAGCAATGCAGGAATACGAAGAGCAGCTGAAAGAAAATGCCAGCGAGGGAAAA
  GGACCAGGGAAAAGGAGAGGTCCAACAATCAAGATATCCGGAGTTCAGGTTAATGTGAAA
  TCCATTATCCAACATGAAGAGGAGTTTGAGATGCTGCATAAATCTATCCCTGTGGACCCT
  GAAGAAAAAAAAAAATACTGCTTAACCTGTCGTGTCAAAGCTGCACATTTTGATGTAGAG
  TGGGGGGTGGAAGATGATTCTCGCCTGTTGCTGGGGATTTATGAACATGGCTATGGAAAC
  TGGGAGTTAATTAAAACAGACCCAGAGCTTAAATTAACTGACAAAATTCTGCCGGTGGAG
  ACAGATAAAAAGCCTCAGGGGAAGCAGCTACAGACCCGAGCGGATTACTTGTTGAAGCTG
  CTCAGAAAGGGTCTGGAGAAGAAGGGGGCTGTGACAGGTGGGGAAGAGGCCAAATTAAAG
  AAGCGGAAGCCTCGGGTAAAGAAGGAAAACAAAGTGCCCAGGCTGAAAGAGGAGCATGGA
  ATTGAGCTTTCATCTCCTAGGCATTCAGATAATCCATCAGAAGAGGGAGAAGTGAAAGAT
  GATGGCTTGGAAAAAAGTCCAATGAAAAAAAAACAGAAGAAGAAAGAGAACAAGGAGAAC
  AAGGAGAAACAAATGAGTTCTAGGAAAGACAAAGAAGGGGACAAGGAAAGAAAGAAGTCA
  AAAGATAAGAAAGAGAAGCCTAAAAGTGGTGATGCCAAATCTTCGAGTAAATCAAAGCGA
  TCTCAGGGTCCTGTCCATATTACAGCAGGAAGTGAACCTGTCCCCATTGGAGAGGATGAG
  GATGATGATCTGGACCAGGAGACATTCAGCATATGTAAGGAGAGGATGAGGCCCGTGAAA
  AAGGCACTGAAACAGCTCGACAAACCTGACAAGGGGCTCAACGTGCAAGAACAGCTGGAA
  CACACCCGGAACTGCCTGCTGAAAATCGGAGACCGGATAGCCGAGTGCCTTAAAGCCTAC
  TCAGATCAGGAGCACATCAAACTCTGGAGGAGGAACCTATGGATTTTTGTTTCCAAGTTT
  ACAGAATTTGATGCTCGAAAACTGCATAAGTTATACAAGATGGCTCATAAGAAAAGGTCT
  CAAGAAGAAGAGGAGCAAAAGAAGAAAGACGACGTGACTGGGGGTAAGAAACCATTTCGT
  CCAGAGGCCTCAGGCTCCAGCCGGGACTCTCTGATATCTCAGTCCCATACCTCACACAAC
  CTTCACCCTCAGAAGCCTCATTTGCCTGCCTCCCATGGCCCACAGATGCATGGACACCCA
  AGAGATAACTACAATCACCCCAACAAGAGACACTTCAGTAATGCAGATCGAGGAGACTGG
  CAGAGGGAAAGAAAGTTCAACTATGGTGGTGGCAACAACAATCCACCATGGGGAAGCGAC
  AGGCACCATCAGTATGAGCAGCACTGGTACAAGGACCACCATTATGGGGACCGGCGACAT
  ATGGATGCCCACCGTTCCGGAAGCTATCGACCCAACAACATGTCCAGAAAGAGGCCTTAT
  GACCAGTACAGCAGTGACCGAGACCACCGGGGACACAGAGATTATTATGACAGGCACCAT
  CATGACTCCAAGCGGAGGAGATCCGATGAATTTAGGCCTCAAAATTACCACCAGCAGGAT
  TTCCGACGAATGTCTGATCACCGCCCCGCTATGGGCTACCATGGCCAGGGACCCTCAGAC
  CATTACCGCTCTTTCCACACAGATAAACTGGGGGAATATAAACAGCCTCTACCCCCATTG
  CACCCTGCAGTCTCAGATCCTCGCTCACCCCCTTCTCAGAAATCTCCTCACGATTCCAAG
  TCACCCCTGGATCATAGGTCTCCTTTGGAGAGATCACTAGAACAGAAAAACAACCCAGAT
  TATAACTGGAATGTTCGGAAAACA
  
• Restriction Sites: Please inquire
• ACCN: NM_001271
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271.3, NP_001262.3
• RefSeq Size: 9374 bp
• RefSeq ORF: 5487 bp
• Locus ID: 1106
• UniProt ID: O14647
• Cytogenetics: 15q26.1
• Domains: SNF2_N, CHROMO, DEAD, helicase_C
• Protein Families: Transcription Factors
• Gene Summary: The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).