TRPS1 (NM_014112) Human Untagged Clone

CAT#: SC318587

TRPS1 (untagged)-Human trichorhinophalangeal syndrome I (TRPS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPS1
• Synonyms: GC79; LGCR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318587 representing NM_014112.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTTATGAAGTCAATGCTGGGTATGATTTTACAAATATGGTCCGGAAAAAGAACCCCCCTCTGAGA
  AACGTTGCAAGTGAAGGCGAGGGCCAGATCCTGGAGCCTATAGGTACAGAAAGCAAGGTATCTGGAAAG
  AACAAAGAATTTTCTGCAGATCAGATGTCAGAAAATACGGATCAGAGTGATGCTGCAGAACTAAATCAT
  AAGGAGGAACATAGCTTGCATGTTCAAGATCCATCTTCTAGCAGTAAGAAGGACTTGAAAAGCGCAGTT
  CTGAGTGAGAAGGCTGGCTTCAATTATGAAAGCCCCAGTAAGGGAGGAAACTTTCCCTCCTTTCCGCAT
  GATGAGGTGACAGACAGAAATATGTTGGCTTTCTCATCTCCAGCTGCTGGGGGAGTCTGTGAGCCCTTG
  AAGTCTCCGCAAAGAGCAGAGGCAGATGACCCTCAAGATATGGCCTGCACCCCCTCAGGGGACTCACTG
  GAGACAAAGGAAGATCAGAAGATGTCACCAAAGGCTACAGAGGAAACAGGGCAAGCACAGAGTGGTCAA
  GCCAATTGTCAAGGTTTGAGCCCAGTTTCAGTGGCCTCAAAAAACCCACAAGTGCCTTCAGATGGGGGT
  GTAAGACTGAATAAATCCAAAACTGACTTACTGGTGAATGACAACCCAGACCCGGCACCTCTGTCTCCA
  GAGCTTCAGGACTTTAAATGCAATATCTGTGGATATGGTTACTACGGCAACGACCCCACAGATCTGATT
  AAGCACTTCCGAAAGTATCACTTAGGACTGCATAACCGCACCAGGCAAGATGCTGAGCTGGACAGCAAA
  ATCTTGGCCCTTCATAACATGGTGCAGTTCAGCCATTCCAAAGACTTCCAGAAGGTCAACCGTTCTGTG
  TTTTCTGGTGTGCTGCAGGACATCAATTCTTCAAGGCCTGTTTTACTAAATGGGACCTATGATGTGCAG
  GTGACTTCAGGTGGAACATTCATTGGCATTGGACGGAAAACACCAGATTGCCAAGGGAACACCAAGTAT
  TTCCGCTGTAAATTCTGCAATTTCACTTATATGGGCAACTCATCCACCGAATTAGAACAACATTTTCTT
  CAGACTCACCCAAACAAAATAAAAGCTTCTCTCCCCTCCTCTGAGGTTGCAAAACCTTCAGAGAAAAAC
  TCTAACAAGTCCATCCCTGCACTTCAATCCAGTGATTCTGGAGACTTGGGAAAATGGCAGGACAAGATA
  ACAGTCAAAGCAGGAGATGACACTCCTGTTGGGTACTCAGTGCCCATAAAGCCCCTCGATTCCTCTAGA
  CAAAATGGTACAGAGGCCACCAGTTACTACTGGTGTAAATTTTGTAGTTTCAGCTGTGAGTCATCTAGC
  TCACTTAAACTGCTAGAACATTATGGCAAGCAGCACGGAGCAGTGCAGTCAGGCGGCCTTAATCCAGAG
  TTAAATGATAAGCTTTCCAGGGGCTCTGTCATTAATCAGAATGATCTAGCCAAAAGTTCAGAAGGAGAG
  ACAATGACCAAGACAGACAAGAGCTCGAGTGGGGCTAAAAAGAAGGACTTCTCCAGCAAGGGAGCCGAG
  GATAATATGGTAACGAGCTATAATTGTCAGTTCTGTGACTTCCGATATTCCAAAAGCCATGGCCCTGAT
  GTAATTGTAGTGGGGCCACTTCTCCGTCATTATCAACAGCTCCATAACATTCACAAGTGTACCATTAAA
  CACTGTCCATTCTGTCCCAGAGGACTTTGCAGCCCAGAAAAGCACCTTGGAGAAATTACTTATCCGTTT
  GCTTGTAGAAAAAGTAATTGTTCCCACTGTGCACTCTTGCTTCTGCACTTGTCTCCTGGGGCGGCTGGA
  AGCTCGCGAGTCAAACATCAGTGCCATCAGTGTTCATTCACCACCCCTGACGTAGATGTACTCCTCTTT
  CACTATGAAAGTGTGCATGAGTCCCAAGCATCGGATGTCAAACAAGAAGCAAATCACCTGCAAGGATCG
  GATGGGCAGCAGTCTGTCAAGGAAAGCAAAGAACACTCATGTACCAAATGTGATTTTATTACCCAAGTG
  GAAGAAGAGATTTCCCGACACTACAGGAGAGCACACAGCTGCTACAAATGCCGTCAGTGCAGTTTTACA
  GCTGCCGATACTCAGTCACTACTGGAGCACTTCAACACTGTTCACTGCCAGGAACAGGACATCACTACA
  GCCAACGGCGAAGAGGACGGTCATGCCATATCCACCATCAAAGAGGAGCCCAAAATTGACTTCAGGGTC
  TACAATCTGCTAACTCCAGACTCTAAAATGGGAGAGCCAGTTTCTGAGAGTGTGGTGAAGAGAGAGAAG
  CTGGAAGAGAAGGACGGGCTCAAAGAGAAAGTTTGGACCGAGAGTTCCAGTGATGACCTTCGCAATGTG
  ACTTGGAGAGGGGCAGACATCCTGCGGGGGAGTCCGTCATACACCCAAGCAAGCCTGGGGCTGCTGACG
  CCTGTGTCTGGCACCCAAGAGCAGACAAAGACTCTAAGGGATAGTCCCAATGTGGAGGCCGCCCATCTG
  GCGCGACCTATTTATGGCTTGGCTGTGGAAACCAAGGGATTCCTGCAGGGGGCGCCAGCTGGCGGAGAG
  AAGTCTGGGGCCCTCCCCCAGCAGTATCCTGCATCGGGAGAAAACAAGTCCAAGGATGAATCCCAGTCC
  CTGTTACGGAGGCGTAGAGGCTCCGGTGTTTTTTGTGCCAATTGCCTGACCACAAAGACCTCTCTCTGG
  CGAAAGAATGCAAATGGCGGATATGTATGCAACGCGTGTGGCCTCTACCAGAAGCTTCACTCGACTCCC
  AGGCCTTTAAACATCATTAAACAAAACAACGGTGAGCAGATTATTAGGAGGAGAACAAGAAAGCGCCTT
  AACCCAGAGGCACTTCAGGCTGAGCAGCTCAACAAACAGCAGAGGGGCAGCAATGAGGAGCAAGTCAAT
  GGAAGCCCGTTAGAGAGGAGGTCAGAAGATCATCTAACTGAAAGTCACCAGAGAGAAATTCCACTCCCC
  AGCCTAAGTAAATACGAAGCCCAGGGTTCATTGACTAAAAGCCATTCTGCTCAGCAGCCAGTCCTGGTC
  AGCCAAACTCTGGATATTCACAAAAGGATGCAACCTTTGCACATTCAGATAAAAAGTCCTCAGGAAAGT
  ACTGGAGATCCAGGAAATAGTTCATCCGTATCTGAAGGGAAAGGAAGTTCTGAGAGAGGCAGTCCTATA
  GAAAAGTACATGAGACCTGCGAAACACCCAAATTATTCACCACCAGGCAGCCCTATTGAAAAGTACCAG
  TACCCACTTTTTGGACTTCCCTTTGTACATAATGACTTCCAGAGTGAAGCTGATTGGCTGCGGTTCTGG
  AGTAAATATAAGCTCTCCGTTCCTGGGAATCCGCACTACTTGAGTCACGTGCCTGGCCTACCAAATCCT
  TGCCAAAACTATGTGCCTTATCCCACCTTCAATCTGCCTCCTCATTTTTCAGCTGTTGGATCAGACAAT
  GACATTCCTCTAGATTTGGCGATCAAGCATTCCAGACCTGGGCCAACTGCAAACGGTGCCTCCAAGGAG
  AAAACGAAGGCACCACCAAATGTAAAAAATGAAGGTCCCTTGAATGTAGTAAAAACAGAGAAAGTTGAT
  AGAAGTACTCAAGATGAACTTTCAACAAAATGTGTGCACTGTGGCATTGTCTTTCTGGATGAAGTGATG
  TATGCTTTGCATATGAGTTGCCATGGTGACAGTGGACCTTTCCAGTGCAGCATATGCCAGCATCTTTGC
  ACGGACAAATATGACTTCACAACACATATCCAGAGGGGCCTGCATAGGAACAATGCACAAGTGGAAAAA
  AATGGAAAACCTAAAGAGTAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_014112
• Insert Size: 3885 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014112.4
• RefSeq Size: 10017 bp
• RefSeq ORF: 3885 bp
• Locus ID: 7227
• UniProt ID: Q9UHF7
• Cytogenetics: 8q23.3
• Domains: GATA, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 143 kDa
• Gene Summary: This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.