AMOTL2 (NM_016201) Human Untagged Clone

CAT#: SC318267

AMOTL2 (untagged)-Human angiomotin like 2 (AMOTL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMOTL2
• Synonyms: LCCP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318267 representing NM_016201.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGACACTGGAAGACTCCTCGGGGACAGTCCTGCACCGCCTCATCCAGGAGCAGCTGCGCTACGGC
  AACCTGACTGAGACGCGCACGCTGCTAGCCATCCAGCAGCAGGCCCTGAGGGGTGGGGCTGGAACTGGG
  GGTACAGGGAGCCCCCAGGCCTCCCTGGAGATCCTGGCCCCAGAGGACAGTCAGGTGCTGCAGCAGGCC
  ACCAGGCAGGAGCCCCAGGGCCAGGAGCACCAGGGCGGTGAGAACCACCTGGCAGAGAACACCCTCTAC
  CGGCTATGCCCACAGCCCAGCAAGGGAGAGGAGCTGCCCACCTATGAGGAGGCCAAAGCCCACTCGCAG
  TACTATGCGGCCCAGCAGGCAGGGACCCGGCCACATGCGGGGGACCGAGATCCCCGTGGGGCCCCGGGA
  GGCAGTCGGAGGCAGGACGAGGCCCTGCGGGAGCTGAGGCATGGGCACGTGCGCTCGTTGAGTGAACGG
  CTCCTTCAGTTGTCCCTGGAGAGGAACGGCGCCCGGGCCCCCAGCCACATGAGCTCCTCCCACAGCTTC
  CCACAGCTGGCCCGCAACCAGCAGGGCCCCCCACTGAGGGGCCCCCCTGCTGAGGGCCCAGAGTCCCGA
  GGACCCCCACCTCAGTACCCTCATGTTGTACTAGCTCATGAGACCACCACTGCTGTCACTGACCCACGG
  TACCGTGCCCGCGGCAGCCCGCACTTCCAGCATGCTGAAGTCAGGATCCTGCAGGCCCAGGTGCCTCCT
  GTGTTCCTCCAACAGCAGCAGCAGTACCAGTACCTGCAGCAATCTCAGGAGCACCCCCCTCCCCCACAT
  CCAGCTGCTCTCGGCCATGGCCCCCTGAGCTCCCTCAGTCCACCTGCTGTGGAGGGGCCAGTGAGTGCC
  CAGGCCTCCTCAGCCACCTCGGGCAGTGCCCACCTGGCCCAGATGGAGGCCGTGCTGAGGGAGAATGCC
  AGGCTGCAGAGAGACAATGAGCGGCTGCAGAGGGAGCTGGAGAGCTCTGCGGAGAAGGCTGGCCGCATT
  GAGAAGCTGGAAAGCGAAATCCAGCGGCTCTCTGAGGCCCATGAGAGCCTGACCAGAGCCTCCTCCAAG
  CGTGAGGCCCTGGAGAAGACCATGCGGAACAAGATGGACAGTGAAATGAGGAGGCTGCAAGACTTCAAC
  CGGGATCTTAGAGAGAGATTGGAATCTGCAAATCGCCGCCTGGCAAGCAAGACACAGGAGGCCCAGGCC
  GGCAGTCAGGACATGGTGGCCAAGCTGCTTGCTCAGAGCTACGAACAGCAGCAGGAGCAAGAGAAGCTG
  GAGCGAGAGATGGCACTGCTGCGCGGCGCCATCGAGGACCAGCGGCGGCGTGCCGAGCTGCTGGAGCAG
  GCTCTGGGCAATGCGCAGGGCCGGGCAGCTCGAGCCGAAGAGGAGCTGCGCAAGAAGCAGGCCTATGTG
  GAGAAAGTGGAGCGGCTGCAGCAGGCGCTCGGGCAGCTGCAGGCAGCCTGTGAGAAGCGGGAGCAGCTG
  GAGCTGCGTCTGCGGACTCGCCTGGAGCAGGAACTCAAGGCCCTGCGTGCACAGCAGAGACAGGCAGGT
  GCCCCAGGTGGTAGCAGTGGCAGTGGTGGGTCTCCAGAGCTCAGCGCCCTGCGACTGTCAGAACAACTG
  CGAGAGAAGGAGGAGCAGATCCTGGCGCTGGAGGCCGACATGACCAAGTGGGAGCAGAAGTATTTGGAG
  GAACGTGCCATGAGGCAGTTTGCCATGGATGCGGCTGCCACGGCTGCTGCTCAGCGTGACACCACTCTC
  ATCCGACATTCCCCCCAGCCCTCACCCAGCAGCAGCTTCAATGAGGGTCTGCTCACTGGTGGCCACAGG
  CATCAGGAGATGGAAAGCAGGTTAAAGGTGCTCCATGCCCAGATCCTGGAGAAGGATGCAGTGATCAAG
  GTCCTTCAGCAGCGCTCCAGGAGAGACCCTGGCAAGGCCATCCAGGGCTCCCTGCGGCCTGCCAAGTCG
  GTGCCATCTGTTTTCGCGGCTGCGGCAGCAGGAACCCAGGGCTGGCAAGGGCTCTCTTCTAGTGAGCGA
  CAAACAGCAGACGCCCCTGCTCGGCTGACTACAGCAGACAGAGCACCCACAGAGGAGCCAGTGGTCACA
  GCTCCCCCTGCTGCCCATGCCAAACACGGGAGCAGAGATGGGAGCACCCAGACTGAGGGCCCCCCAGAC
  AGCACCTCCACCTGCCTGCCACCGGAGCCTGACAGCCTTCTGGGGTGCAGCAGTAGCCAGAGAGCAGCC
  TCTCTGGACTCTGTAGCTACATCCAGAGTCCAGGACTTGTCAGACATGGTGGAGATACTGATCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016201
• Insert Size: 2343 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016201.3
• RefSeq Size: 5008 bp
• RefSeq ORF: 2343 bp
• Locus ID: 51421
• UniProt ID: Q9Y2J4
• Cytogenetics: 3q22.2
• MW: 85.8 kDa
• Gene Summary: Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR, uses an alternate in-frame splice site in the 3' coding region and initiates translation at a downstream start site, compared to variant 1. It encodes isoform 2, which is shorter at the N-terminus and contains an additional internal amino acid compared to isoform 1.