DYNC2I2 (NM_052844) Human Untagged Clone

CAT#: SC317908

WDR34 (untagged)-Human WD repeat domain 34 (WDR34)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYNC2I2
• Synonyms: bA216B9.3; CFAP133; DIC5; FAP133; SRTD11; WDR34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317908 representing NM_052844.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAACCCGCGCGCAGCCGGGGCCACTCAGCCAGGCGGGAAGCGCTGGTGTTGCGGCGCTGGCGACA
  GTCGGGGTTGCGAGCGGCCCGGGGCCGGGGCGGCCAGGGCCGCTGCAGGACGAGACCCTGGGTGTGGCG
  TCCGTGCCCTCGCAGTGGAGGGCCGTCCAGGGCATCCGCTGGGAGACGAAAAGTTGCCAGACGGCCAGC
  ATTGCCACTGCCAGTGCATCCGCCCAGGCCAGGAATCATGTGGACGCCCAGGTGCAGACGGAGGCCCCC
  GTGCCTGTCAGCGTGCAGCCCCCGTCCCAGTATGACATACCCAGGCTCGCAGCCTTTCTTCGGAGAGTG
  GAGGCCATGGTCATCCGAGAGCTGAACAAGAATTGGCAGAGCCACGCGTTTGATGGCTTCGAGGTGAAC
  TGGACCGAGCAGCAGCAGATGGTGTCTTGTCTGTATACCCTGGGCTACCCGCCAGCCCAAGCGCAGGGT
  CTGCATGTGACCAGCATCTCCTGGAACTCCACTGGCTCTGTGGTGGCCTGTGCCTACGGCCGGCTGGAC
  CATGGGGACTGGAGCACGCTTAAGTCCTTCGTGTGTGCCTGGAACCTGGACCGGCGAGACCTGCGTCCC
  CAGCAGCCGTCGGCCGTGGTGGAGGTCCCCAGCGCTGTCCTGTGTCTGGCCTTCCACCCCACGCAGCCC
  TCCCACGTCGCAGGAGGGCTGTACAGTGGTGAGGTGTTGGTGTGGGACCTGAGCCGTCTTGAGGACCCG
  CTGCTGTGGCGCACAGGCCTGACGGATGACACCCACACAGACCCTGTGTCCCAGGTGGTGTGGCTGCCC
  GAGCCTGGGCACAGCCACCGCTTCCAGGTGCTGAGTGTGGCCACCGACGGGAAGGTGCTACTCTGGCAG
  GGCATCGGGGTAGGCCAGCTGCAGCTCACAGAGGGCTTCGCCCTGGTCATGCAGCAGCTGCCACGGAGC
  ACCAAGCTCAAGAAGCATCCCCGCGGGGAGACCGAGGTGGGCGCCACGGCAGTGGCCTTCTCCAGCTTT
  GACCCTAGGCTGTTCATTCTGGGCACGGAAGGCGGCTTCCCGCTCAAGTGTTCCCTGGCAGCTGGAGAG
  GCAGCCCTCACGCGGATGCCCAGCTCCGTGCCCCTGCGGGCCCCAGCACAGTTTACCTTCTCCCCCCAC
  GGCGGTCCCATCTACTCTGTGAGCTGTTCCCCCTTCCACAGGAATCTCTTCCTGAGCGCTGGGACTGAC
  GGGCATGTCCACCTGTACTCCATGCTGCAGGCCCCTCCCTTGACTTCGCTGCAGCTCTCCCTCAAGTAT
  CTGTTTGCTGTGCGCTGGTCCCCAGTGCGGCCCTTGGTTTTTGCAGCTGCCTCTGGGAAAGGTGACGTG
  CAGCTGTTTGATCTCCAGAAAAGCTCCCAGAAACCCACAGTTTTGATCAAGCAAACCCAGGATGAAAGC
  CCTGTCTACTGTCTGGAGTTCAACAGCCAGCAGACTCAGCTCTTGGCTGCGGGCGATGCCCAGGGCACA
  GTGAAGGTGTGGCAGCTGAGCACAGAGTTCACGGAACAAGGGCCCCGGGAAGCTGAGGACCTGGACTGC
  CTGGCAGCAGAGGTGGCGGCCTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_052844
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052844.3
• RefSeq Size: 1818 bp
• RefSeq ORF: 1611 bp
• Locus ID: 89891
• UniProt ID: Q96EX3
• Cytogenetics: 9q34.11
• Domains: WD40
• MW: 57.8 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Defects in this gene are a cause of short-rib thoracic dysplasia 11 with or without polydactyly. [provided by RefSeq, Mar 2014]