PKNOX2 (NM_022062) Human Untagged Clone

SKU SC317778

PKNOX2 (untagged)-Human PBX/knotted 1 homeobox 2 (PKNOX2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PKNOX2
• Synonyms: PREP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317778 representing NM_022062.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGATGCAACATGCCTCCCCAGCCCCCGCTCTGACGATGATGGCCACGCAGAATGTCCCGCCCCCACCC; TACCAGGACAGCCCACAGATGACGGCAACCGCCCAGCCACCCTCCAAGGCCCAGGCTGTCCACATCTCT; GCCCCCTCAGCTGCTGCCAGCACACCTGTGCCCAGTGCCCCCATCGACCCCCAGGCCCAGCTGGAGGCT; GACAAGCGAGCTGTATACAGGCACCCTCTTTTCCCGCTCCTGACGCTGCTGTTTGAGAAATGTGAACAG; GCCACCCAGGGCTCTGAGTGCATCACCTCCGCCAGCTTTGATGTGGACATCGAGAACTTTGTCCACCAG; CAGGAACAGGAGCACAAACCCTTCTTCAGCGATGACCCAGAACTGGACAATCTGATGGTGAAGGCAATC; CAGGTCCTGAGAATCCACCTGCTGGAGCTGGAGAAAGTCAATGAACTCTGCAAGGACTTTTGTAACCGT; TACATCACCTGCCTCAAAACCAAGATGCACAGCGACAACCTGCTCAGGAATGATCTAGGGGGGCCCTAC; TCCCCCAACCAGCCCTCCATCAACCTTCACTCACAGGACCTCCTGCAGAATTCCCCCAATTCCATGTCC; GGAGTCTCCAATAACCCCCAGGGGATTGTGGTCCCAGCCTCAGCGCTCCAGCAGGGCAACATCGCCATG; ACAACCGTCAACTCACAAGTTGTGTCAGGTGGAGCCTTATACCAACCGGTTACCATGGTAACCTCCCAG; GGTCAGGTGGTCACCCAAGCAATCCCCCAGGGAGCCATCCAGATCCAGAACACACAGGTTAACCTTGAC; CTCACCTCCCTCCTGGACAATGAGGATAAGAAGTCCAAGAACAAACGAGGAGTCTTGCCCAAGCATGCC; ACCAATATAATGCGTTCTTGGCTCTTCCAGCATCTCATGCACCCCTACCCCACGGAGGATGAGAAGAGG; CAGATCGCAGCCCAGACCAACCTCACCCTCCTGCAAGTAAACAACTGGTTCATCAATGCCCGGAGGCGC; ATCCTGCAGCCCATGCTTGATGCCAGCAACCCAGATCCTGCCCCCAAAGCCAAGAAGATCAAGTCTCAG; CACCGGCCCACCCAAAGATTCTGGCCCAACTCCATCGCTGCGGGGGTGCTGCAGCAGCAGGGCGGTGCC; CCAGGGACAAACCCCGATGGTTCCATCAACTTGGACAACCTGCAGTCCCTGTCCTCAGACAGTGCCACC; ATGGCCATGCAGCAGGCTATGATGGCTGCACACGATGACTCATTGGATGGGACAGAAGAAGAGGATGAG; GATGAGATGGAAGAGGAGGAGGAGGAGGAGCTGGAGGAGGAGGTCGACGAGCTGCAGACGACAAATGTC; AGCGACCTGGGCTTGGAACACAGTGACTCCCTGGAGTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_022062
• Insert Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022062.2
• RefSeq Size: 3730 bp
• RefSeq ORF: 1419 bp
• Locus ID: 63876
• UniProt ID: Q96KN3
• Cytogenetics: 11q24.2
• Protein Families: Transcription Factors
• MW: 52 kDa
• Summary: Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct 2009]